This is a Validated Antibody Database (VAD) review about human PAR-2, based on 18 published articles (read how Labome selects the articles), using PAR-2 antibody in all methods. It is aimed to help Labome visitors find the most suited PAR-2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PAR-2 synonym: GPR11; PAR2

Knockout validation
Invitrogen
mouse monoclonal (SAM11)
  • western blot knockout validation; mouse; 1:400; fig 1
  • immunohistochemistry; mouse; 1:500; fig 1
In order to test if proteinase-activated receptor-2 modulates the deleterious effects of IL-1beta in ischemic retinopathies, Invitrogen PAR-2 antibody (Invitrogen, clone SAM11) was used in western blot knockout validation on mouse samples at 1:400 (fig 1) and in immunohistochemistry on mouse samples at 1:500 (fig 1). Am J Pathol (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (SAM11)
  • immunohistochemistry; human; 1:50; fig 4a
Santa Cruz Biotechnology PAR-2 antibody (Santa Cruz Biotechnology, sc-13504) was used in immunohistochemistry on human samples at 1:50 (fig 4a). Cancers (Basel) (2021) ncbi
mouse monoclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7a
  • western blot; human; loading ...; fig 1c
Santa Cruz Biotechnology PAR-2 antibody (SantaCruz, SAM11) was used in immunohistochemistry - paraffin section on mouse samples (fig 7a) and in western blot on human samples (fig 1c). Oncotarget (2017) ncbi
mouse monoclonal (SAM11)
  • western blot; human; loading ...; fig 1c
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7a
Santa Cruz Biotechnology PAR-2 antibody (SantaCruz, SAM11) was used in western blot on human samples (fig 1c) and in immunohistochemistry - paraffin section on mouse samples (fig 7a). Oncotarget (2017) ncbi
mouse monoclonal (SAM11)
  • western blot; human; fig 2b
Santa Cruz Biotechnology PAR-2 antibody (Santa Cruz, sc-13504) was used in western blot on human samples (fig 2b). Front Pharmacol (2016) ncbi
mouse monoclonal (SAM11)
  • flow cytometry; human; 1:100; fig 2 b-e
  • immunohistochemistry; human; fig 1a
  • western blot; human; 1:100; fig 2
Santa Cruz Biotechnology PAR-2 antibody (santa cruz, sc-13504) was used in flow cytometry on human samples at 1:100 (fig 2 b-e), in immunohistochemistry on human samples (fig 1a) and in western blot on human samples at 1:100 (fig 2). J Biol Chem (2016) ncbi
mouse monoclonal (SAM11)
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
Santa Cruz Biotechnology PAR-2 antibody (Santa Cruz, sc-13504) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 2). PLoS ONE (2015) ncbi
mouse monoclonal (SAM11)
  • flow cytometry; human; fig 2
  • immunocytochemistry; human; fig 2
Santa Cruz Biotechnology PAR-2 antibody (Santa Cruz, sc-13504) was used in flow cytometry on human samples (fig 2) and in immunocytochemistry on human samples (fig 2). Physiol Rep (2015) ncbi
mouse monoclonal (SAM11)
  • western blot; human; 1:1000; loading ...; fig 1b
Santa Cruz Biotechnology PAR-2 antibody (Santa Cruz, sc-13504) was used in western blot on human samples at 1:1000 (fig 1b). Cell Death Dis (2014) ncbi
mouse monoclonal (SAM11)
  • immunohistochemistry; mouse; 1:250
  • immunohistochemistry; rat; 1:250
Santa Cruz Biotechnology PAR-2 antibody (Santa Cruz Biotechnology, SC-13504) was used in immunohistochemistry on mouse samples at 1:250 and in immunohistochemistry on rat samples at 1:250. J Comp Neurol (2009) ncbi
Invitrogen
mouse monoclonal (SAM11)
  • immunocytochemistry; human; fig 4
In order to assess the effect of polyinosinic-polycytidylic acid on the barrier function and tight junction integrity of primary human lung microvascular endothelial cells, Invitrogen PAR-2 antibody (Invitrogen, 35-2300) was used in immunocytochemistry on human samples (fig 4). PLoS ONE (2016) ncbi
mouse monoclonal (SAM11)
  • immunohistochemistry - paraffin section; human
  • immunocytochemistry; human
  • western blot; human; 1:100
Invitrogen PAR-2 antibody (Zymed Laboratories, SAM11) was used in immunohistochemistry - paraffin section on human samples , in immunocytochemistry on human samples and in western blot on human samples at 1:100. Hum Pathol (2015) ncbi
mouse monoclonal (SAM11)
  • western blot knockout validation; mouse; 1:400; fig 1
  • immunohistochemistry; mouse; 1:500; fig 1
In order to test if proteinase-activated receptor-2 modulates the deleterious effects of IL-1beta in ischemic retinopathies, Invitrogen PAR-2 antibody (Invitrogen, clone SAM11) was used in western blot knockout validation on mouse samples at 1:400 (fig 1) and in immunohistochemistry on mouse samples at 1:500 (fig 1). Am J Pathol (2015) ncbi
mouse monoclonal (SAM11)
  • immunohistochemistry - paraffin section; human; fig 2
In order to examine expression and biological activity of PAR-1 and PAR-2 in normal and pathological cutaneous scars, Invitrogen PAR-2 antibody (Zymed, clone SAM11) was used in immunohistochemistry - paraffin section on human samples (fig 2). J Pathol (2007) ncbi
mouse monoclonal (SAM11)
  • immunohistochemistry - paraffin section; human; 1:50; tbl 1
In order to determine the expression of PAR-1, PAR-2, MMP-2, MMP-9, COX-1, and COX-2 in patients with orofacial granulomatosis, Invitrogen PAR-2 antibody (Zymed, SAM 11) was used in immunohistochemistry - paraffin section on human samples at 1:50 (tbl 1). Oral Dis (2007) ncbi
mouse monoclonal (SAM11)
  • immunocytochemistry; human; fig 4
In order to look for a cellular receptor for Bacteroides fragilis toxin on intestinal epithelial cells, Invitrogen PAR-2 antibody (Zymed, SAM11) was used in immunocytochemistry on human samples (fig 4). Infect Immun (2006) ncbi
Abcam
domestic rabbit monoclonal (EPR13675)
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 1a
  • western blot; rat; loading ...; fig 1c
Abcam PAR-2 antibody (Abcam, ab180953) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 1a) and in western blot on rat samples (fig 1c). Aging (Albany NY) (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4b
Abcam PAR-2 antibody (Abcam, ab124227) was used in western blot on human samples (fig 4b). Mol Syst Biol (2017) ncbi
LifeSpan Biosciences
mouse monoclonal (SAM11)
  • immunohistochemistry; mouse
  • western blot; mouse
  • immunohistochemistry; human; loading ...; fig 1a
LifeSpan Biosciences PAR-2 antibody (LSBio, SAM11) was used in immunohistochemistry on mouse samples , in western blot on mouse samples and in immunohistochemistry on human samples (fig 1a). Am J Physiol Gastrointest Liver Physiol (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D61D5)
  • western blot; rat; 1:1000; loading ...; fig 5a
Cell Signaling Technology PAR-2 antibody (Cell Signaling, 6976) was used in western blot on rat samples at 1:1000 (fig 5a). Biosci Rep (2020) ncbi
Articles Reviewed
  1. Tu N, Inoue K, Chen E, Anderson B, Sawicki C, Scheff N, et al. Cathepsin S Evokes PAR2-Dependent Pain in Oral Squamous Cell Carcinoma Patients and Preclinical Mouse Models. Cancers (Basel). 2021;13: pubmed publisher
  2. Yan S, Ding H, Peng J, Wang X, Pang C, Wei J, et al. Down-regulation of protease-activated receptor 2 ameliorated osteoarthritis in rats through regulation of MAPK/NF-κB signaling pathway in vivo and in vitro. Biosci Rep. 2020;40: pubmed publisher
  3. Huang X, Ni B, Xi Y, Chu X, Zhang R, You H. Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis. Aging (Albany NY). 2019;11:12532-12545 pubmed publisher
  4. Nag J, Kancharla A, Maoz M, Turm H, Agranovich D, Gupta C, et al. Low-density lipoprotein receptor-related protein 6 is a novel coreceptor of protease-activated receptor-2 in the dynamics of cancer-associated ?-catenin stabilization. Oncotarget. 2017;8:38650-38667 pubmed publisher
  5. Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gandia J, et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol. 2017;13:918 pubmed publisher
  6. Indrakusuma I, Romacho T, Eckel J. Protease-Activated Receptor 2 Promotes Pro-Atherogenic Effects through Transactivation of the VEGF Receptor 2 in Human Vascular Smooth Muscle Cells. Front Pharmacol. 2016;7:497 pubmed publisher
  7. Huang L, Stuart C, Takeda K, D Agnillo F, Golding B. Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5. PLoS ONE. 2016;11:e0160875 pubmed publisher
  8. Nasri I, Bonnet D, Zwarycz B, d Aldebert E, Khou S, Mezghani Jarraya R, et al. PAR2-dependent activation of GSK3? regulates the survival of colon stem/progenitor cells. Am J Physiol Gastrointest Liver Physiol. 2016;311:G221-36 pubmed publisher
  9. Johnson J, Miller D, Jiang R, Liu Y, Shi Z, Tarwater L, et al. Protease-activated Receptor-2 (PAR-2)-mediated Nf-κB Activation Suppresses Inflammation-associated Tumor Suppressor MicroRNAs in Oral Squamous Cell Carcinoma. J Biol Chem. 2016;291:6936-45 pubmed publisher
  10. Lidfeldt J, Bendahl P, Forsare C, Malmström P, Fernö M, Belting M. Protease Activated Receptors 1 and 2 Correlate Differently with Breast Cancer Aggressiveness Depending on Tumor ER Status. PLoS ONE. 2015;10:e0134932 pubmed publisher
  11. Al Eryani K, Cheng J, Abe T, Maruyama S, Yamazaki M, Babkair H, et al. Protease-activated receptor 2 modulates proliferation and invasion of oral squamous cell carcinoma cells. Hum Pathol. 2015;46:991-9 pubmed publisher
  12. Asokananthan N, Lan R, Graham P, Bakker A, Tokanović A, Stewart G. Activation of protease-activated receptors (PARs)-1 and -2 promotes alpha-smooth muscle actin expression and release of cytokines from human lung fibroblasts. Physiol Rep. 2015;3: pubmed publisher
  13. Sitaras N, Rivera J, Noueihed B, Bien Aimé M, Zaniolo K, Omri S, et al. Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2. Am J Pathol. 2015;185:581-95 pubmed publisher
  14. Chen K, Wang C, Tsai M, Wu C, Yang H, Chen L, et al. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death Dis. 2014;5:e1244 pubmed publisher
  15. Amadesi S, Grant A, Cottrell G, Vaksman N, Poole D, Rozengurt E, et al. Protein kinase D isoforms are expressed in rat and mouse primary sensory neurons and are activated by agonists of protease-activated receptor 2. J Comp Neurol. 2009;516:141-56 pubmed publisher
  16. Materazzi S, Pellerito S, Di Serio C, Paglierani M, Naldini A, Ardinghi C, et al. Analysis of protease-activated receptor-1 and -2 in human scar formation. J Pathol. 2007;212:440-9 pubmed
  17. Ketabchi S, Massi D, Ficarra G, Rubino I, Franchi A, Paglierani M, et al. Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis. Oral Dis. 2007;13:419-25 pubmed
  18. Wu S, Shin J, Zhang G, Cohen M, Franco A, Sears C. The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor. Infect Immun. 2006;74:5382-90 pubmed