This is a Validated Antibody Database (VAD) review about human PAR-1, based on 13 published articles (read how Labome selects the articles), using PAR-1 antibody in all methods. It is aimed to help Labome visitors find the most suited PAR-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PAR-1 synonym: CF2R; HTR; PAR-1; PAR1; TR

Santa Cruz Biotechnology
mouse monoclonal (ATAP2)
  • western blot; human; 1:500; loading ...; fig st3
In order to study the DNA damage response in senescent epithelial cells, Santa Cruz Biotechnology PAR-1 antibody (Santa Cruz, sc-13503) was used in western blot on human samples at 1:500 (fig st3). Nat Commun (2016) ncbi
mouse monoclonal (ATAP2)
  • immunohistochemistry - paraffin section; human; 1:150; tbl 2
Santa Cruz Biotechnology PAR-1 antibody (Santa Cruz, sc-13503) was used in immunohistochemistry - paraffin section on human samples at 1:150 (tbl 2). PLoS ONE (2015) ncbi
mouse monoclonal (ATAP2)
  • western blot; rat; 1:200
Santa Cruz Biotechnology PAR-1 antibody (Santa Cruz Biotechnology, sc-13503) was used in western blot on rat samples at 1:200. Int J Mol Med (2015) ncbi
mouse monoclonal (ATAP2)
  • flow cytometry; human; fig 2
  • immunocytochemistry; human; fig 2
Santa Cruz Biotechnology PAR-1 antibody (Santa Cruz, sc-13503) was used in flow cytometry on human samples (fig 2) and in immunocytochemistry on human samples (fig 2). Physiol Rep (2015) ncbi
mouse monoclonal (ATAP2)
  • flow cytometry; human
  • western blot; human
In order to investigate the effect of cell senescence on tissue factor, Santa Cruz Biotechnology PAR-1 antibody (Santa Cruz, sc-13503) was used in flow cytometry on human samples and in western blot on human samples . Mech Ageing Dev (2014) ncbi
mouse monoclonal (ATAP2)
  • immunocytochemistry; human
In order to study the mechanism by which prohibitin modulates PAR1 internalization and degradation, Santa Cruz Biotechnology PAR-1 antibody (Santa Cruz, ATAP2) was used in immunocytochemistry on human samples . Biochim Biophys Acta (2014) ncbi
Invitrogen
mouse monoclonal (ATAP2)
  • blocking or activating experiments; human; fig 3
In order to elucidate the mechanism by which APC promotes angiogenesis and breast cancer invasion, Invitrogen PAR-1 antibody (Zymed, atap2) was used in blocking or activating experiments on human samples (fig 3). Exp Cell Res (2010) ncbi
mouse monoclonal (ATAP2)
  • immunohistochemistry - paraffin section; human; fig 2
In order to examine expression and biological activity of PAR-1 and PAR-2 in normal and pathological cutaneous scars, Invitrogen PAR-1 antibody (Zymed, clone ATAP2) was used in immunohistochemistry - paraffin section on human samples (fig 2). J Pathol (2007) ncbi
mouse monoclonal (ATAP2)
  • immunohistochemistry - paraffin section; human; 1:50; tbl 1
In order to determine the expression of PAR-1, PAR-2, MMP-2, MMP-9, COX-1, and COX-2 in patients with orofacial granulomatosis, Invitrogen PAR-1 antibody (Zymed, ATAP 2) was used in immunohistochemistry - paraffin section on human samples at 1:50 (tbl 1). Oral Dis (2007) ncbi
mouse monoclonal (ATAP2)
  • blocking or activating experiments; human; 10 ug/ml; fig 6
In order to examine the role of activated protein C in cell chemotaxis and invasion, Invitrogen PAR-1 antibody (Zymed, atap2) was used in blocking or activating experiments on human samples at 10 ug/ml (fig 6). Exp Cell Res (2007) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig e3k
Abcam PAR-1 antibody (Abcam, ab32611) was used in western blot on human samples at 1:1000 (fig e3k). Nat Metab (2021) ncbi
Beckman Coulter
mouse monoclonal (WEDE15)
  • immunohistochemistry - paraffin section; human
Beckman Coulter PAR-1 antibody (Beckman Coulter, IM2085) was used in immunohistochemistry - paraffin section on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (WEDE15)
  • immunocytochemistry; human; 1:100
Beckman Coulter PAR-1 antibody (Beckman Coulter, IM2085) was used in immunocytochemistry on human samples at 1:100. Methods Enzymol (2014) ncbi
Articles Reviewed
  1. Perry E, Bennett C, Luo C, Balsa E, Jedrychowski M, O Malley K, et al. Tetracyclines promote survival and fitness in mitochondrial disease models. Nat Metab. 2021;3:33-42 pubmed publisher
  2. Nassour J, Martien S, Martin N, Deruy E, Tomellini E, Malaquin N, et al. Defective DNA single-strand break repair is responsible for senescence and neoplastic escape of epithelial cells. Nat Commun. 2016;7:10399 pubmed publisher
  3. Lidfeldt J, Bendahl P, Forsare C, Malmström P, Fernö M, Belting M. Protease Activated Receptors 1 and 2 Correlate Differently with Breast Cancer Aggressiveness Depending on Tumor ER Status. PLoS ONE. 2015;10:e0134932 pubmed publisher
  4. Jin S, Liu Y, Deng S, Liao L, Lin T, Ning Q, et al. Neuroprotective effects of activated protein C on intrauterine inflammation-induced neonatal white matter injury are associated with the downregulation of fibrinogen-like protein 2/fibroleukin prothrombinase and the inhibition of pro-inflammatory cyt. Int J Mol Med. 2015;35:1199-212 pubmed publisher
  5. Asokananthan N, Lan R, Graham P, Bakker A, Tokanović A, Stewart G. Activation of protease-activated receptors (PARs)-1 and -2 promotes alpha-smooth muscle actin expression and release of cytokines from human lung fibroblasts. Physiol Rep. 2015;3: pubmed publisher
  6. Kurz D, Payeli S, Greutert H, Briand Schumacher S, Luscher T, Tanner F. Epigenetic regulation of tissue factor inducibility in endothelial cell senescence. Mech Ageing Dev. 2014;140:1-9 pubmed publisher
  7. Wang Y, Guo X, Li S, Zhao Y, Liu Z, Lee W, et al. Prohibitin is involved in the activated internalization and degradation of protease-activated receptor 1. Biochim Biophys Acta. 2014;1843:1393-401 pubmed publisher
  8. Mogami H, Keller P, Shi H, Word R. Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth. J Biol Chem. 2014;289:13295-307 pubmed publisher
  9. Grimsey N, Lin H, Trejo J. Endosomal signaling by protease-activated receptors. Methods Enzymol. 2014;535:389-401 pubmed publisher
  10. Gramling M, Beaulieu L, Church F. Activated protein C enhances cell motility of endothelial cells and MDA-MB-231 breast cancer cells by intracellular signal transduction. Exp Cell Res. 2010;316:314-28 pubmed publisher
  11. Materazzi S, Pellerito S, Di Serio C, Paglierani M, Naldini A, Ardinghi C, et al. Analysis of protease-activated receptor-1 and -2 in human scar formation. J Pathol. 2007;212:440-9 pubmed
  12. Ketabchi S, Massi D, Ficarra G, Rubino I, Franchi A, Paglierani M, et al. Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis. Oral Dis. 2007;13:419-25 pubmed
  13. Beaulieu L, Church F. Activated protein C promotes breast cancer cell migration through interactions with EPCR and PAR-1. Exp Cell Res. 2007;313:677-87 pubmed