This is a Validated Antibody Database (VAD) review about human PAK1, based on 24 published articles (read how Labome selects the articles), using PAK1 antibody in all methods. It is aimed to help Labome visitors find the most suited PAK1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PAK1 synonym: IDDMSSD; PAKalpha; alpha-PAK; p65-PAK

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Cell Signaling Technology PAK1 antibody (Cell signaling, 2602) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2a). Front Physiol (2021) ncbi
Invitrogen
domestic rabbit polyclonal
Invitrogen PAK1 antibody (Thermo Fisher, PA5-12844) was used . J Biol Chem (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Invitrogen PAK1 antibody (Biosource, 44-940G) was used in western blot on human samples at 1:1000 (fig st1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; African green monkey; 1:150; fig s8
In order to determine regulation of spine structural plasticity and memory and learning by the calcium sensor Copine-6, Invitrogen PAK1 antibody (Life Technologies, 44940G) was used in immunocytochemistry on African green monkey samples at 1:150 (fig s8). Nat Commun (2016) ncbi
domestic rabbit polyclonal
In order to assess the contribution of tissue damage and extracellular matrix fragmentation during inflammation, Invitrogen PAK1 antibody (Invitrogen, 44940G) was used . Sci Adv (2015) ncbi
domestic rabbit polyclonal
In order to study the molecular mechanisms underlying malignant changes of meningioma cells, Invitrogen PAK1 antibody (Invitrogen, 44940G) was used . Oncotarget (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-6)
  • western blot; mouse; loading ...; fig 5D
Santa Cruz Biotechnology PAK1 antibody (Santa Cruz, sc-166887) was used in western blot on mouse samples (fig 5D). elife (2017) ncbi
mouse monoclonal (D-8)
  • immunohistochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 3b
Santa Cruz Biotechnology PAK1 antibody (Santa Cruz Biotechnology, sc-166174) was used in immunohistochemistry on human samples (fig 2a) and in western blot on human samples (fig 3b). Oncotarget (2016) ncbi
mouse monoclonal (A-6)
  • western blot; human; 1:400
Santa Cruz Biotechnology PAK1 antibody (Santa Cruz, sc-166887) was used in western blot on human samples at 1:400. PLoS ONE (2016) ncbi
mouse monoclonal (A-6)
  • immunoprecipitation; human; fig 6
  • western blot; human; fig 5
Santa Cruz Biotechnology PAK1 antibody (Santa Cruz Biotechnologies, sc-166887) was used in immunoprecipitation on human samples (fig 6) and in western blot on human samples (fig 5). Blood (2015) ncbi
Abcam
domestic rabbit monoclonal (EP656Y)
  • western blot; rat; 1:1000; loading ...; fig 4a
  • western blot; mouse; 1:1000; loading ...; fig 4c
Abcam PAK1 antibody (Abcam, ab40795) was used in western blot on rat samples at 1:1000 (fig 4a) and in western blot on mouse samples at 1:1000 (fig 4c). J Biol Chem (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Cell Signaling Technology PAK1 antibody (Cell signaling, 2602) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2a). Front Physiol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4e
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2602) was used in western blot on human samples at 1:1000 (fig 4e). Front Cell Dev Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4e
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2601) was used in western blot on human samples at 1:1000 (fig 4e). Front Cell Dev Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4f
Cell Signaling Technology PAK1 antibody (Cell Signaling Tech, 2601S) was used in western blot on mouse samples (fig 4f). Sci Transl Med (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6e
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2604) was used in western blot on mouse samples at 1:1000 (fig 6e). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6f
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2606) was used in western blot on mouse samples at 1:1000 (fig 6f). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6e
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2602) was used in western blot on mouse samples at 1:1000 (fig 6e). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; fig 3g
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2601S) was used in western blot on human samples at 1:500 (fig 3g). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; fig 3g
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2604) was used in western blot on human samples at 1:500 (fig 3g). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4d
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2604) was used in western blot on mouse samples at 1:1000 (fig 4d). Aging Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4d
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2605) was used in western blot on mouse samples at 1:1000 (fig 4d). Aging Cell (2018) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2602) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 3a
  • western blot; human; 1:1000; loading ...; fig 2c
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2606) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 3a) and in western blot on human samples at 1:1000 (fig 2c). Genes Dev (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig 2c
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2602) was used in western blot on human samples at 1:2000 (fig 2c). Genes Dev (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 9e
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2606) was used in western blot on human samples (fig 9e). J Clin Invest (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 6G
Cell Signaling Technology PAK1 antibody (Cell Signaling, 2606) was used in western blot on mouse samples (fig 6G). elife (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6d
In order to investigate whether kidney podocytes exhibit stiffness sensitivity, Cell Signaling Technology PAK1 antibody (Cell Signaling, 2601) was used in western blot on human samples (fig 6d). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology PAK1 antibody (Cell Signaling, 2606) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology PAK1 antibody (Cell Signaling, 2604) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000
Cell Signaling Technology PAK1 antibody (Cell Signalling, 2604) was used in western blot on mouse samples at 1:1000. Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
Cell Signaling Technology PAK1 antibody (Cell Signalling, 2602) was used in western blot on mouse samples at 1:1000 (fig 5). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3a
In order to investigate the interaction between MUC13 and HER2, Cell Signaling Technology PAK1 antibody (Cell Signaling, 2602) was used in western blot on human samples (fig 3a). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3a
In order to investigate the interaction between MUC13 and HER2, Cell Signaling Technology PAK1 antibody (Cell Signaling, 2605) was used in western blot on human samples (fig 3a). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3b
In order to research the role of PCBP1/HNRNP E1 in chromosomal integrity, Cell Signaling Technology PAK1 antibody (Cell Signaling Technology, 2601) was used in western blot on mouse samples (fig 3b). Mol Cancer Res (2016) ncbi
MilliporeSigma
mouse monoclonal (PK-18)
  • immunohistochemistry; mouse; 1:500; fig 6
MilliporeSigma PAK1 antibody (Sigma, P3237) was used in immunohistochemistry on mouse samples at 1:500 (fig 6). J Cell Sci (2016) ncbi
Articles Reviewed
  1. Barbé C, Loumaye A, Lause P, Ritvos O, Thissen J. p21-Activated Kinase 1 Is Permissive for the Skeletal Muscle Hypertrophy Induced by Myostatin Inhibition. Front Physiol. 2021;12:677746 pubmed publisher
  2. Shi X, Wen Z, Wang Y, Liu Y, Shi K, Jiu Y. Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration. Front Cell Dev Biol. 2021;9:665919 pubmed publisher
  3. Jaynes J, Sable R, Ronzetti M, Bautista W, Knotts Z, Abisoye Ogunniyan A, et al. Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses. Sci Transl Med. 2020;12: pubmed publisher
  4. Laurin M, Gomez N, Levorse J, Sendoel A, Sribour M, Fuchs E. An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis. elife. 2019;8: pubmed publisher
  5. Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, et al. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nat Commun. 2019;10:2350 pubmed publisher
  6. Baglietto Vargas D, Prieto G, Limon A, Forner S, Rodriguez Ortiz C, Ikemura K, et al. Impaired AMPA signaling and cytoskeletal alterations induce early synaptic dysfunction in a mouse model of Alzheimer's disease. Aging Cell. 2018;17:e12791 pubmed publisher
  7. Ge J, Burnier L, Adamopoulou M, Kwa M, Schaks M, Rottner K, et al. RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells. J Biol Chem. 2018;293:9358-9369 pubmed publisher
  8. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  9. Yue X, Zhang C, Zhao Y, Liu J, Lin A, Tan V, et al. Gain-of-function mutant p53 activates small GTPase Rac1 through SUMOylation to promote tumor progression. Genes Dev. 2017;31:1641-1654 pubmed publisher
  10. Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  11. Chang J, Ni Y, Han L, Sinnett Smith J, Jacamo R, Rey O, et al. Protein kinase D1 (PKD1) phosphorylation on Ser203 by type I p21-activated kinase (PAK) regulates PKD1 localization. J Biol Chem. 2017;292:9523-9539 pubmed publisher
  12. Jeannot P, Nowosad A, Perchey R, Callot C, Bennana E, Katsube T, et al. p27Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway. elife. 2017;6: pubmed publisher
  13. Hu M, Azeloglu E, Ron A, Tran Ba K, Calizo R, Tavassoly I, et al. A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction. Sci Rep. 2017;7:43934 pubmed publisher
  14. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  15. Martin K, Pritchett J, Llewellyn J, Mullan A, Athwal V, Dobie R, et al. PAK proteins and YAP-1 signalling downstream of integrin beta-1 in myofibroblasts promote liver fibrosis. Nat Commun. 2016;7:12502 pubmed publisher
  16. Yuan W, Guo Y, Li X, Deng M, Shen Z, Bo C, et al. MicroRNA-126 inhibits colon cancer cell proliferation and invasion by targeting the chemokine (C-X-C motif) receptor 4 and Ras homolog gene family, member A, signaling pathway. Oncotarget. 2016;7:60230-60244 pubmed publisher
  17. Khan S, Sikander M, Ebeling M, Ganju A, Kumari S, Yallapu M, et al. MUC13 interaction with receptor tyrosine kinase HER2 drives pancreatic ductal adenocarcinoma progression. Oncogene. 2017;36:491-500 pubmed publisher
  18. Reinhard J, Kriz A, Galic M, Angliker N, Rajalu M, Vogt K, et al. The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun. 2016;7:11613 pubmed publisher
  19. Link L, Howley B, Hussey G, Howe P. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res. 2016;14:634-46 pubmed publisher
  20. Wang Y, Gratzke C, Tamalunas A, Wiemer N, Ciotkowska A, Rutz B, et al. P21-Activated Kinase Inhibitors FRAX486 and IPA3: Inhibition of Prostate Stromal Cell Growth and Effects on Smooth Muscle Contraction in the Human Prostate. PLoS ONE. 2016;11:e0153312 pubmed publisher
  21. Jimeno D, Gómez C, Calzada N, de la Villa P, Lillo C, Santos E. RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors. J Cell Sci. 2016;129:729-42 pubmed publisher
  22. Hahn C, Scott D, Xu X, Roda M, Payne G, Wells J, et al. The matrikine N-α-PGP couples extracellular matrix fragmentation to endothelial permeability. Sci Adv. 2015;1: pubmed
  23. Chow H, Dong B, Duron S, Campbell D, Ong C, Hoeflich K, et al. Group I Paks as therapeutic targets in NF2-deficient meningioma. Oncotarget. 2015;6:1981-94 pubmed
  24. Badolia R, Manne B, Dangelmaier C, Chernoff J, Kunapuli S. Gq-mediated Akt translocation to the membrane: a novel PIP3-independent mechanism in platelets. Blood. 2015;125:175-84 pubmed publisher