This is a Validated Antibody Database (VAD) review about human PAI-1, based on 26 published articles (read how Labome selects the articles), using PAI-1 antibody in all methods. It is aimed to help Labome visitors find the most suited PAI-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PAI-1 synonym: PAI; PAI-1; PAI1; PLANH1; plasminogen activator inhibitor 1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Santa Cruz Biotechnology
mouse monoclonal (C-9)
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology PAI-1 antibody (Santa Cruz, sc-5297) was used in western blot on human samples (fig 2a). Sci Rep (2017) ncbi
mouse monoclonal (C-9)
  • western blot; human; 1:500; loading ...; fig 2c
Santa Cruz Biotechnology PAI-1 antibody (SantaCruz, sc-5297) was used in western blot on human samples at 1:500 (fig 2c). Oncol Lett (2016) ncbi
mouse monoclonal (C-9)
  • immunocytochemistry; human; fig s1a
  • western blot; human; fig 3a
In order to elucidate the mechanism of p53-dependent upregulation of OCT4A and p21Cip1, Santa Cruz Biotechnology PAI-1 antibody (Santa cruz, sc-5297) was used in immunocytochemistry on human samples (fig s1a) and in western blot on human samples (fig 3a). Cell Cycle (2015) ncbi
mouse monoclonal (C-9)
  • immunocytochemistry; pig; fig 1
Santa Cruz Biotechnology PAI-1 antibody (Santa cruz, sc-5297) was used in immunocytochemistry on pig samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (C-9)
  • western blot; human
Santa Cruz Biotechnology PAI-1 antibody (Santa Cruz Biotechnology, sc-5297) was used in western blot on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (C-9)
  • immunocytochemistry; human; 1:100
Santa Cruz Biotechnology PAI-1 antibody (Santa Cruz, sc-5297) was used in immunocytochemistry on human samples at 1:100. J Biol Chem (2013) ncbi
Abcam
rabbit polyclonal
  • western blot; pig; fig 5
Abcam PAI-1 antibody (Abcam, ab66705) was used in western blot on pig samples (fig 5). J Am Heart Assoc (2016) ncbi
mouse monoclonal (1D5)
  • western blot; mouse; fig 1
Abcam PAI-1 antibody (Abcam, ab125687) was used in western blot on mouse samples (fig 1). Iran J Basic Med Sci (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Abcam PAI-1 antibody (Abcam, ab66705) was used in western blot on human samples (fig 1). Sci Rep (2015) ncbi
goat polyclonal
  • western blot; human; loading ...; fig 1a
Abcam PAI-1 antibody (Abcam, ab31280) was used in western blot on human samples (fig 1a). Oncotarget (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:1000
Abcam PAI-1 antibody (Abcam, ab66705) was used in immunohistochemistry on human samples at 1:1000. FEBS Lett (2015) ncbi
mouse monoclonal (1D5)
  • western blot; human; 1:1000
Abcam PAI-1 antibody (Abcam, ab125687) was used in western blot on human samples at 1:1000. FEBS Lett (2015) ncbi
mouse monoclonal (1D5)
  • western blot; human; 1:2000; fig 4
Abcam PAI-1 antibody (Abcam, ab125687) was used in western blot on human samples at 1:2000 (fig 4). Mol Med Rep (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Abcam PAI-1 antibody (Abcam, ab66705) was used in western blot on human samples at 1:1000. J Mol Endocrinol (2014) ncbi
Abnova
mouse monoclonal (1D5)
  • immunohistochemistry - paraffin section; human; 1:200; fig 1a
  • western blot; human; 1:1000
Abnova PAI-1 antibody (Abnova, 1D5) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1a) and in western blot on human samples at 1:1000. Oncotarget (2015) ncbi
R&D Systems
goat polyclonal
  • immunohistochemistry - paraffin section; human
In order to evaluate a genomic strategy for the evaluation of transforming growth factor-beta in breast cancer, R&D Systems PAI-1 antibody (R&D Systems, AF1786) was used in immunohistochemistry - paraffin section on human samples . Breast Cancer Res (2014) ncbi
Invitrogen
mouse monoclonal (1D5)
  • western blot; human; 1:600
In order to evaluate the LPA-producing enzyme autotaxin and LPAR3 as potential therapeutic targets in breast cancer patients, Invitrogen PAI-1 antibody (Zymed, 1D5) was used in western blot on human samples at 1:600. Tumour Biol (2012) ncbi
BD Biosciences
mouse monoclonal (41/PAI-1)
  • other; human; loading ...; fig 4c
BD Biosciences PAI-1 antibody (BD Biosciences, 612024) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
mouse monoclonal (41/PAI-1)
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., BD Biosciences PAI-1 antibody (BD Biosciences, 612024) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
mouse monoclonal (41/PAI-1)
  • reverse phase protein lysate microarray; human; loading ...; fig 3a
In order to describe the features of 228 primary cervical cancers, BD Biosciences PAI-1 antibody (BD Biosciences, 612024) was used in reverse phase protein lysate microarray on human samples (fig 3a). Nature (2017) ncbi
mouse monoclonal (41/PAI-1)
  • western blot; human; loading ...; fig 7e
In order to propose that DLX1 modulates FOXM1 signaling to promote cancer aggressiveness via TGF-beta/SMAD4 signaling in high-grade serous ovarian cancer cells, BD Biosciences PAI-1 antibody (BD Transduction Laboratories, 612024) was used in western blot on human samples (fig 7e). Oncogene (2017) ncbi
mouse monoclonal (41/PAI-1)
  • western blot; human; loading ...; fig 6b
In order to study how CUB domain-containing protein1 contributes to metastasis and drug resistance, BD Biosciences PAI-1 antibody (BD Transduction Laboratories, 612024) was used in western blot on human samples (fig 6b). Breast Cancer Res (2016) ncbi
mouse monoclonal (41/PAI-1)
  • immunohistochemistry - paraffin section; human; 1:200; fig s1
In order to utilize ovarian cancer xenograft models to study dynamic modulation of phosphoprotein expression, BD Biosciences PAI-1 antibody (BD sciences, P612024) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig s1). BMC Cancer (2016) ncbi
mouse monoclonal (41/PAI-1)
  • western blot; human; 1:5000
In order to show that tamoxifen prevents myofibroblast differentiation, BD Biosciences PAI-1 antibody (BD Biosciences, 612025) was used in western blot on human samples at 1:5000. J Cell Physiol (2015) ncbi
mouse monoclonal (41/PAI-1)
  • western blot; human; fig 11
BD Biosciences PAI-1 antibody (BD Transduction, 612025) was used in western blot on human samples (fig 11). Nucleic Acids Res (2015) ncbi
EMD Millipore
mouse monoclonal
  • other; human; loading ...; fig s1
In order to investigate the mechanisms of toxicity by the negative allosteric modulators of metabotropic glutamate receptor 5, EMD Millipore PAI-1 antibody (Calbiochem, IM29L-100UG) was used in other on human samples (fig s1). Cell Chem Biol (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to study the effect of cotransin treatment on protein synthesis, EMD Millipore PAI-1 antibody (Millipore, 09-726) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
Articles Reviewed
  1. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  2. Shah F, Stepan A, O Mahony A, Velichko S, Folias A, Houle C, et al. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators. Cell Chem Biol. 2017;24:858-869.e5 pubmed publisher
  3. Yan Y, Zhao W, Huang Y, Tong H, Xia Y, Jiang Q, et al. Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Sci Rep. 2017;7:46276 pubmed publisher
  4. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  5. . Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
  6. Chan D, Hui W, Wang J, Yung M, Hui L, Qin Y, et al. DLX1 acts as a crucial target of FOXM1 to promote ovarian cancer aggressiveness by enhancing TGF-β/SMAD4 signaling. Oncogene. 2017;36:1404-1416 pubmed publisher
  7. LAW M, Ferreira R, Davis B, Higgins P, Kim J, Castellano R, et al. CUB domain-containing protein 1 and the epidermal growth factor receptor cooperate to induce cell detachment. Breast Cancer Res. 2016;18:80 pubmed publisher
  8. Ding Y, Zhang H, Lu A, Zhou Z, Zhong M, Shen D, et al. Effect of urokinase-type plasminogen activator system in gastric cancer with peritoneal metastasis. Oncol Lett. 2016;11:4208-4216 pubmed
  9. Eirin A, Ebrahimi B, Kwon S, Fiala J, Williams B, Woollard J, et al. Restoration of Mitochondrial Cardiolipin Attenuates Cardiac Damage in Swine Renovascular Hypertension. J Am Heart Assoc. 2016;5: pubmed publisher
  10. Liang Q, Wang B, Pang L, Wang Y, Zheng M, Wang Q, et al. Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice. Iran J Basic Med Sci. 2016;19:43-8 pubmed
  11. Koussounadis A, Langdon S, Um I, Kay C, Francis K, Harrison D, et al. Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models. BMC Cancer. 2016;16:205 pubmed publisher
  12. Pavon M, Arroyo Solera I, Tellez Gabriel M, Leon X, Virós D, Lopez M, et al. Enhanced cell migration and apoptosis resistance may underlie the association between high SERPINE1 expression and poor outcome in head and neck carcinoma patients. Oncotarget. 2015;6:29016-33 pubmed publisher
  13. Huna A, Salmina K, Erenpreisa J, Vazquez Martin A, Krigerts J, Inashkina I, et al. Role of stress-activated OCT4A in the cell fate decisions of embryonal carcinoma cells treated with etoposide. Cell Cycle. 2015;14:2969-84 pubmed publisher
  14. Carthy J, Sundqvist A, Heldin A, van Dam H, Kletsas D, Heldin C, et al. Tamoxifen Inhibits TGF-β-Mediated Activation of Myofibroblasts by Blocking Non-Smad Signaling Through ERK1/2. J Cell Physiol. 2015;230:3084-92 pubmed publisher
  15. Rojas Fernandez A, Herhaus L, MacArtney T, Lachaud C, Hay R, Sapkota G. Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9. Sci Rep. 2015;5:9811 pubmed publisher
  16. Liu X, Chen Z, Xu C, Leng X, Cao H, Ouyang G, et al. Repression of hypoxia-inducible factor α signaling by Set7-mediated methylation. Nucleic Acids Res. 2015;43:5081-98 pubmed publisher
  17. Zhang W, Pei Y, Zhong L, Wen B, Cao S, Han J. Pluripotent and Metabolic Features of Two Types of Porcine iPSCs Derived from Defined Mouse and Human ES Cell Culture Conditions. PLoS ONE. 2015;10:e0124562 pubmed publisher
  18. Klein W, Westendorf C, Schmidt A, Conill Cortés M, Rutz C, Blohs M, et al. Defining a conformational consensus motif in cotransin-sensitive signal sequences: a proteomic and site-directed mutagenesis study. PLoS ONE. 2015;10:e0120886 pubmed publisher
  19. Falkenberg N, Anastasov N, Schaub A, Radulovic V, Schmitt M, Magdolen V, et al. Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221. Oncotarget. 2015;6:8103-14 pubmed
  20. Li C, Zhu H, Bai W, Su L, Liu J, Cai W, et al. MiR-10a and miR-181c regulate collagen type I generation in hypertrophic scars by targeting PAI-1 and uPA. FEBS Lett. 2015;589:380-9 pubmed publisher
  21. Huang W, Li L, Tian X, Yan J, Yang X, Wang X, et al. Astragalus and Paeoniae Radix Rubra extract (APE) inhibits hepatic stellate cell activation by modulating transforming growth factor-β/Smad pathway. Mol Med Rep. 2015;11:2569-77 pubmed publisher
  22. Sato M, Kadota M, Tang B, Yang H, Yang Y, Shan M, et al. An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-? in human breast cancer. Breast Cancer Res. 2014;16:R57 pubmed publisher
  23. Tian L, Zhang L, Liu J, Guo T, Gao C, Ni J. Effects of TSH on the function of human umbilical vein endothelial cells. J Mol Endocrinol. 2014;52:215-22 pubmed publisher
  24. Feng Y, Wu H, Xu Y, Zhang Z, Liu T, Lin X, et al. Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-? signaling. J Biol Chem. 2014;289:2072-83 pubmed publisher
  25. Sharma A, Diecke S, Zhang W, Lan F, He C, Mordwinkin N, et al. The role of SIRT6 protein in aging and reprogramming of human induced pluripotent stem cells. J Biol Chem. 2013;288:18439-47 pubmed publisher
  26. Popnikolov N, Dalwadi B, Thomas J, Johannes G, Imagawa W. Association of autotaxin and lysophosphatidic acid receptor 3 with aggressiveness of human breast carcinoma. Tumour Biol. 2012;33:2237-43 pubmed publisher