This is a Validated Antibody Database (VAD) review about human PADI4, based on 12 published articles (read how Labome selects the articles), using PADI4 antibody in all methods. It is aimed to help Labome visitors find the most suited PADI4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PADI4 synonym: PAD; PAD4; PADI5; PDI4; PDI5

Abcam
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Abcam PADI4 antibody (Abcam, ab50247) was used in western blot on human samples (fig 2a). Mol Cell (2019) ncbi
mouse monoclonal (OTI4H5)
  • western blot; human; 1:2000; fig s11
Abcam PADI4 antibody (Abcam, ab128086) was used in western blot on human samples at 1:2000 (fig s11). Nat Commun (2016) ncbi
mouse monoclonal (OTI4H5)
  • western blot; human; fig 3
In order to test if dying neutrophils release peptidyl arginine deiminase, which results in citrullination of antigens relevant to rheumatoid arthritis, Abcam PADI4 antibody (Abcam, ab128086) was used in western blot on human samples (fig 3). Arthritis Res Ther (2015) ncbi
mouse monoclonal (OTI4H5)
  • immunohistochemistry - frozen section; human; loading ...; fig 4
Abcam PADI4 antibody (Abcam, ab128086) was used in immunohistochemistry - frozen section on human samples (fig 4). Arthritis Rheumatol (2015) ncbi
mouse monoclonal (OTI4H5)
  • immunocytochemistry; human; 1:100
Abcam PADI4 antibody (Abcam, ab128086) was used in immunocytochemistry on human samples at 1:100. Respir Res (2015) ncbi
mouse monoclonal (OTI4H5)
  • immunocytochemistry; human; 1:100
Abcam PADI4 antibody (Abcam, ab128086) was used in immunocytochemistry on human samples at 1:100. PLoS ONE (2014) ncbi
mouse monoclonal (OTI4H5)
  • immunohistochemistry - paraffin section; human; fig 1c
Abcam PADI4 antibody (Abcam, ab128086) was used in immunohistochemistry - paraffin section on human samples (fig 1c). J Thromb Haemost (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:30; loading ...; fig 1
In order to explore the synovial protein targets of peptidylarginine deiminases, Invitrogen PADI4 antibody (Pierce, PA5-12236) was used in immunohistochemistry - paraffin section on human samples at 1:30 (fig 1). Eur J Rheumatol (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 10 ug/ml; loading ...; fig 1f
In order to study the contribution of neutrophil extracellular traps to pulmonary hypertension, Invitrogen PADI4 antibody (ThermoScientific, PA5-22317) was used in immunohistochemistry - paraffin section on human samples at 10 ug/ml (fig 1f). Arterioscler Thromb Vasc Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; rhesus macaque; 1:10; fig 9
In order to examine the role of B cells in the common marmoset and rhesus monkey models of experimental autoimmune encephalomyelitis, Invitrogen PADI4 antibody (Invitrogen, PA5-22317) was used in immunocytochemistry on rhesus macaque samples at 1:10 (fig 9). J Immunol (2016) ncbi
Proteintech Group
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 4b
  • immunohistochemistry; human; 1:200; loading ...; fig 3b
Proteintech Group PADI4 antibody (ProteinTech, 17373-1) was used in immunocytochemistry on human samples at 1:200 (fig 4b) and in immunohistochemistry on human samples at 1:200 (fig 3b). Arthritis Res Ther (2020) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
  • immunocytochemistry; human; fig 3
  • western blot; human; 1:1000; fig 3
MilliporeSigma PADI4 antibody (Sigma, P4874) was used in chromatin immunoprecipitation on human samples (fig 4), in immunocytochemistry on human samples (fig 3) and in western blot on human samples at 1:1000 (fig 3). Oncotarget (2016) ncbi
Articles Reviewed
  1. Trimova G, Yamagata K, Iwata S, Hirata S, Zhang T, Uemura F, et al. Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages. Arthritis Res Ther. 2020;22:24 pubmed publisher
  2. Sharma P, Lioutas A, Fernandez Fuentes N, Quilez J, Carbonell Caballero J, Wright R, et al. Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Mol Cell. 2019;73:84-96.e7 pubmed publisher
  3. Badillo Soto M, Rodríguez Rodríguez M, Pérez Pérez M, Daza Benítez L, Bollain Y Goytia J, Carrillo Jiménez M, et al. Potential protein targets of the peptidylarginine deiminase 2 and peptidylarginine deiminase 4 enzymes in rheumatoid synovial tissue and its possible meaning. Eur J Rheumatol. 2016;3:44-49 pubmed
  4. Aldabbous L, Abdul Salam V, McKinnon T, Duluc L, Pepke Zaba J, Southwood M, et al. Neutrophil Extracellular Traps Promote Angiogenesis: Evidence From Vascular Pathology in Pulmonary Hypertension. Arterioscler Thromb Vasc Biol. 2016;36:2078-87 pubmed publisher
  5. Jagessar S, Holtman I, Hofman S, Morandi E, Heijmans N, Laman J, et al. Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte Glycoprotein. J Immunol. 2016;197:1074-88 pubmed publisher
  6. Katkar G, Sundaram M, NaveenKumar S, Swethakumar B, Sharma R, Paul M, et al. NETosis and lack of DNase activity are key factors in Echis carinatus venom-induced tissue destruction. Nat Commun. 2016;7:11361 pubmed publisher
  7. Blachère N, Parveen S, Fak J, Frank M, Orange D. Inflammatory but not apoptotic death of granulocytes citrullinates fibrinogen. Arthritis Res Ther. 2015;17:369 pubmed publisher
  8. Song G, Shi L, Guo Y, Yu L, Wang L, Zhang X, et al. A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells. Oncotarget. 2016;7:3144-57 pubmed publisher
  9. Spengler J, Lugonja B, Ytterberg A, Zubarev R, Creese A, Pearson M, et al. Release of Active Peptidyl Arginine Deiminases by Neutrophils Can Explain Production of Extracellular Citrullinated Autoantigens in Rheumatoid Arthritis Synovial Fluid. Arthritis Rheumatol. 2015;67:3135-45 pubmed publisher
  10. Grabcanovic Musija F, Obermayer A, Stoiber W, Krautgartner W, Steinbacher P, Winterberg N, et al. Neutrophil extracellular trap (NET) formation characterises stable and exacerbated COPD and correlates with airflow limitation. Respir Res. 2015;16:59 pubmed publisher
  11. Obermayer A, Stoiber W, Krautgartner W, Klappacher M, Kofler B, Steinbacher P, et al. New aspects on the structure of neutrophil extracellular traps from chronic obstructive pulmonary disease and in vitro generation. PLoS ONE. 2014;9:e97784 pubmed publisher
  12. Savchenko A, Martinod K, Seidman M, Wong S, Borissoff J, Piazza G, et al. Neutrophil extracellular traps form predominantly during the organizing stage of human venous thromboembolism development. J Thromb Haemost. 2014;12:860-70 pubmed publisher