This is a Validated Antibody Database (VAD) review about human P2Y12, based on 12 published articles (read how Labome selects the articles), using P2Y12 antibody in all methods. It is aimed to help Labome visitors find the most suited P2Y12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
P2Y12 synonym: ADPG-R; BDPLT8; HORK3; P2T(AC); P2Y(12)R; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999

Abcam
domestic rabbit monoclonal (EPR18611)
  • western blot; human; loading ...; fig 1a
Abcam P2Y12 antibody (Abcam, EPR18611) was used in western blot on human samples (fig 1a). Cancers (Basel) (2020) ncbi
domestic rabbit monoclonal (EPR18611)
  • immunohistochemistry - frozen section; rat; 1:150; loading ...; fig 2
Abcam P2Y12 antibody (Abcam, ab184411) was used in immunohistochemistry - frozen section on rat samples at 1:150 (fig 2). Cell Physiol Biochem (2018) ncbi
domestic rabbit monoclonal (EPR18611)
  • western blot; human; loading ...; fig s4
Abcam P2Y12 antibody (Abcam, EPR18611) was used in western blot on human samples (fig s4). Cancers (Basel) (2017) ncbi
domestic rabbit monoclonal (EPR18611)
  • western blot; rat; 1:1000; fig 2c
Abcam P2Y12 antibody (Abcam, ab184411) was used in western blot on rat samples at 1:1000 (fig 2c). J Neuroinflammation (2017) ncbi
Invitrogen
domestic rabbit recombinant (4H5L19)
  • western blot; human; loading ...; fig s1a
Invitrogen P2Y12 antibody (ThermoFischer, 4H5L19) was used in western blot on human samples (fig s1a). Cancers (Basel) (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 3s1d
Invitrogen P2Y12 antibody (ThermoFisher, PA5-77671) was used in immunohistochemistry on mouse samples at 1:100 (fig 3s1d). elife (2019) ncbi
BioLegend
mouse monoclonal (S16001E)
  • flow cytometry; human; fig 7a
BioLegend P2Y12 antibody (Biolegend, 392103) was used in flow cytometry on human samples (fig 7a). Front Cell Neurosci (2021) ncbi
Alomone Labs
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2j
Alomone Labs P2Y12 antibody (Alomone Labs, APR-012) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 2j). Front Immunol (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1
Alomone Labs P2Y12 antibody (Alomone Labs, APR-012) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1). Int J Mol Sci (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 1b
Alomone Labs P2Y12 antibody (Alomone Labs, APR-020) was used in immunocytochemistry on human samples at 1:100 (fig 1b). Mol Psychiatry (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; tbl 1
In order to study P2Y12 expression in human brain tissue, Alomone Labs P2Y12 antibody (Alomone, APR-020) was used in immunohistochemistry - paraffin section on human samples (tbl 1). Glia (2017) ncbi
OriGene
domestic rabbit polyclonal (polyclonal)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
  • western blot; mouse; loading ...; fig 3e
In order to evaluate the molecular mechanisms of how vessel wall P2Y12 mediates vascular smooth muscle cell migration and promotes the progression of atherosclerosis, OriGene P2Y12 antibody (OriGene, TA328648) was used in immunohistochemistry - paraffin section on mouse samples (fig 2a) and in western blot on mouse samples (fig 3e). Arterioscler Thromb Vasc Biol (2017) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; tbl 1
In order to study P2Y12 expression in human brain tissue, MilliporeSigma P2Y12 antibody (Sigma, HPA013796) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 1). Glia (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:100; fig 4a
In order to test if the microglial P2Y12 receptor in the trigeminal spinal subnucleus caudalis is involved in intraoral cancer pain, MilliporeSigma P2Y12 antibody (Sigma, P4871) was used in immunohistochemistry - free floating section on rat samples at 1:100 (fig 4a). J Dent Res (2016) ncbi
Articles Reviewed
  1. Bodnar B, Zhang Y, Liu J, Lin Y, Wang P, Wei Z, et al. Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells. Front Cell Neurosci. 2021;15:682272 pubmed publisher
  2. Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
  3. Walker D, Tang T, Mendsaikhan A, Tooyama I, Serrano G, Sue L, et al. Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer's Disease Brains. Int J Mol Sci. 2020;21: pubmed publisher
  4. Elaskalani O, Domenichini A, Abdol Razak N, E Dye D, Falasca M, Metharom P. Antiplatelet Drug Ticagrelor Enhances Chemotherapeutic Efficacy by Targeting the Novel P2Y12-AKT Pathway in Pancreatic Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
  5. Ma W, Silverman S, Zhao L, Villasmil R, Campos M, Amaral J, et al. Absence of TGFβ signaling in retinal microglia induces retinal degeneration and exacerbates choroidal neovascularization. elife. 2019;8: pubmed publisher
  6. Sheng X, Wang J, Guo J, Xu Y, Jiang H, Zheng C, et al. Effects of Baicalin on Diabetic Cardiac Autonomic Neuropathy Mediated by the P2Y12 Receptor in Rat Stellate Ganglia. Cell Physiol Biochem. 2018;46:986-998 pubmed publisher
  7. Elaskalani O, Falasca M, Moran N, Berndt M, Metharom P. The Role of Platelet-Derived ADP and ATP in Promoting Pancreatic Cancer Cell Survival and Gemcitabine Resistance. Cancers (Basel). 2017;9: pubmed publisher
  8. Niu J, Huang D, Zhou R, Yue M, Xu T, Yang J, et al. Activation of dorsal horn cannabinoid CB2 receptor suppresses the expression of P2Y12 and P2Y13 receptors in neuropathic pain rats. J Neuroinflammation. 2017;14:185 pubmed publisher
  9. Niu X, Pi S, Baral S, Xia Y, He Q, Li Y, et al. P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis. Arterioscler Thromb Vasc Biol. 2017;37:515-524 pubmed publisher
  10. Sellgren C, Sheridan S, Gracias J, Xuan D, Fu T, Perlis R. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry. 2017;22:170-177 pubmed publisher
  11. Mildner A, Huang H, Radke J, Stenzel W, Priller J. P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases. Glia. 2017;65:375-387 pubmed publisher
  12. Tamagawa T, Shinoda M, Honda K, Furukawa A, Kaji K, Nagashima H, et al. Involvement of Microglial P2Y12 Signaling in Tongue Cancer Pain. J Dent Res. 2016;95:1176-82 pubmed publisher