This is a Validated Antibody Database (VAD) review about human OTOF, based on 8 published articles (read how Labome selects the articles), using OTOF antibody in all methods. It is aimed to help Labome visitors find the most suited OTOF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
OTOF synonym: AUNB1; DFNB6; DFNB9; FER1L2; NSRD9

Abcam
mouse monoclonal (13A9)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3c
Abcam OTOF antibody (Abcam, ab53233) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 3c). Development (2018) ncbi
mouse monoclonal (13A9)
  • immunohistochemistry; mouse; fig ev2a
Abcam OTOF antibody (Abcam, ab53233) was used in immunohistochemistry on mouse samples (fig ev2a). EMBO Rep (2017) ncbi
mouse monoclonal (13A9)
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 4b
In order to characterize two new families with heterozygous truncating DIAPH1 mutations, Abcam OTOF antibody (Abcam, ab53233) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig 4b). Clin Genet (2017) ncbi
mouse monoclonal (13A9)
  • immunohistochemistry; mouse; fig 4a
Abcam OTOF antibody (Abcam, ab53233) was used in immunohistochemistry on mouse samples (fig 4a). J Cell Sci (2015) ncbi
mouse monoclonal (13A9)
  • immunohistochemistry; mouse; 1:200
Abcam OTOF antibody (AbCam, ab53233) was used in immunohistochemistry on mouse samples at 1:200. J Neurosci (2014) ncbi
mouse monoclonal (13A9)
  • immunocytochemistry; mouse; 1:100
In order to study the conversion inner border cells and inner phalangeal cells to inner hair cells, Abcam OTOF antibody (Abcam, ab53233) was used in immunocytochemistry on mouse samples at 1:100. PLoS ONE (2014) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (HCS-1)
  • immunohistochemistry; zebrafish ; 1:750; loading ...; fig 1a
Developmental Studies Hybridoma Bank OTOF antibody (Developmental Studies Hybridoma Bank, HCS-1) was used in immunohistochemistry on zebrafish samples at 1:750 (fig 1a). Mol Biol Cell (2021) ncbi
mouse monoclonal (HCS-1)
  • immunohistochemistry; chicken; 1:250; loading ...; fig 3d
Developmental Studies Hybridoma Bank OTOF antibody (Developmental Studies Hybridoma Bank, HCS-1) was used in immunohistochemistry on chicken samples at 1:250 (fig 3d). elife (2019) ncbi
Articles Reviewed
  1. Manchanda A, Bonventre J, Bugel S, Chatterjee P, Tanguay R, Johnson C. Truncation of the otoferlin transmembrane domain alters the development of hair cells and reduces membrane docking. Mol Biol Cell. 2021;32:1293-1305 pubmed publisher
  2. Zhu Y, Scheibinger M, Ellwanger D, Krey J, Choi D, Kelly R, et al. Single-cell proteomics reveals changes in expression during hair-cell development. elife. 2019;8: pubmed publisher
  3. Zhang H, Pan H, Zhou C, Wei Y, Ying W, Li S, et al. Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Development. 2018;145: pubmed publisher
  4. Vogl C, Butola T, Haag N, Hausrat T, Leitner M, Moutschen M, et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep. 2017;18:2015-2029 pubmed publisher
  5. Neuhaus C, Lang Roth R, Zimmermann U, Heller R, Eisenberger T, Weikert M, et al. Extension of the clinical and molecular phenotype of DIAPH1-associated autosomal dominant hearing loss (DFNA1). Clin Genet. 2017;91:892-901 pubmed publisher
  6. Vogl C, Cooper B, Neef J, Wojcik S, Reim K, Reisinger E, et al. Unconventional molecular regulation of synaptic vesicle replenishment in cochlear inner hair cells. J Cell Sci. 2015;128:638-44 pubmed publisher
  7. Vincent P, Bouleau Y, Safieddine S, Petit C, Dulon D. Exocytotic machineries of vestibular type I and cochlear ribbon synapses display similar intrinsic otoferlin-dependent Ca2+ sensitivity but a different coupling to Ca2+ channels. J Neurosci. 2014;34:10853-69 pubmed publisher
  8. Liu Z, Fang J, Dearman J, Zhang L, Zuo J. In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression. PLoS ONE. 2014;9:e89377 pubmed publisher