This is a Validated Antibody Database (VAD) review about human ORC2, based on 8 published articles (read how Labome selects the articles), using ORC2 antibody in all methods. It is aimed to help Labome visitors find the most suited ORC2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ORC2 synonym: ORC2L; origin recognition complex subunit 2; origin recognition complex protein 2 homolog; origin recognition complex, subunit 2 homolog

Knockout validation
Santa Cruz Biotechnology
rat monoclonal (3G6)
  • western blot knockout validation; human; loading ...; fig 1d
In order to demonstrate a role for CDC6 in cell survival and DNA replication, Santa Cruz Biotechnology ORC2 antibody (Santa Cruz, sc-32734) was used in western blot knockout validation on human samples (fig 1d). elife (2016) ncbi
Santa Cruz Biotechnology
rat monoclonal (3G6)
  • western blot knockout validation; human; loading ...; fig 1d
In order to demonstrate a role for CDC6 in cell survival and DNA replication, Santa Cruz Biotechnology ORC2 antibody (Santa Cruz, sc-32734) was used in western blot knockout validation on human samples (fig 1d). elife (2016) ncbi
rat monoclonal (3G6)
  • western blot; human; 1:1500
Santa Cruz Biotechnology ORC2 antibody (Santa Cruz, sc-32734) was used in western blot on human samples at 1:1500. PLoS ONE (2016) ncbi
rat monoclonal (3G6)
  • western blot; human; 1:1000; fig s7
Santa Cruz Biotechnology ORC2 antibody (Santa Cruz Biotechnology, sc-32734) was used in western blot on human samples at 1:1000 (fig s7). Nat Commun (2016) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig s6
Bethyl ORC2 antibody (Bethyl Laboratories, A302-734) was used in western blot on human samples (fig s6). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human; fig s6
Bethyl ORC2 antibody (Bethyl Laboratories, A302-735) was used in western blot on human samples (fig s6). PLoS ONE (2015) ncbi
Abcam
mouse monoclonal (SB46)
  • flow cytometry; human; 1:200; loading ...
In order to determine specific residues on four MCM helicase subunits that show hyperphosphorylation upon RIF1 depletion, Abcam ORC2 antibody (Abcam, ab31930) was used in flow cytometry on human samples at 1:200. EMBO Rep (2017) ncbi
Cell Signaling Technology
rat monoclonal (3G6)
  • western blot; human; fig 1
Cell Signaling Technology ORC2 antibody (Cell Signaling Technology, 4736) was used in western blot on human samples (fig 1). Cell Rep (2016) ncbi
rat monoclonal (3G6)
  • western blot; human; 1:1000; fig 1
Cell Signaling Technology ORC2 antibody (Cell Signaling, 4736) was used in western blot on human samples at 1:1000 (fig 1). Nat Commun (2014) ncbi
BD Biosciences
mouse monoclonal (920-4-41)
  • western blot; Rhesus monkey; fig 3
BD Biosciences ORC2 antibody (BD Biosciences, clone 920-4-41) was used in western blot on Rhesus monkey samples (fig 3). J Virol (2010) ncbi
Articles Reviewed
  1. Hiraga S, Ly T, Garzón J, Horejsi Z, Ohkubo Y, Endo A, et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep. 2017;18:403-419 pubmed publisher
  2. Shibata E, Kiran M, Shibata Y, Singh S, Kiran S, Dutta A. Two subunits of human ORC are dispensable for DNA replication and proliferation. elife. 2016;5: pubmed publisher
  3. Huang Y, Amin A, Qin Y, Wang Z, Jiang H, Liang L, et al. A Role of hIPI3 in DNA Replication Licensing in Human Cells. PLoS ONE. 2016;11:e0151803 pubmed publisher
  4. Huang C, Cheng J, Bawa Khalfe T, Yao X, Chin Y, Yeh E. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Cell Rep. 2016;15:147-157 pubmed publisher
  5. Passerini V, Ozeri Galai E, de Pagter M, Donnelly N, Schmalbrock S, Kloosterman W, et al. The presence of extra chromosomes leads to genomic instability. Nat Commun. 2016;7:10754 pubmed publisher
  6. Graindorge D, Martineau S, Machon C, Arnoux P, Guitton J, Francesconi S, et al. Singlet Oxygen-Mediated Oxidation during UVA Radiation Alters the Dynamic of Genomic DNA Replication. PLoS ONE. 2015;10:e0140645 pubmed publisher
  7. Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher
  8. Boichuk S, Hu L, Hein J, Gjoerup O. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J Virol. 2010;84:8007-20 pubmed publisher