This is a Validated Antibody Database (VAD) review about human Nck, based on 7 published articles (read how Labome selects the articles), using Nck antibody in all methods. It is aimed to help Labome visitors find the most suited Nck antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Nck synonym: NCK; NCKalpha; nck-1

Santa Cruz Biotechnology
mouse monoclonal (8.8)
  • western blot; Xenopus laevis; 1:1000; loading ...; fig 3
Santa Cruz Biotechnology Nck antibody (Santa Cruz, sc-20020) was used in western blot on Xenopus laevis samples at 1:1000 (fig 3). PLoS Pathog (2017) ncbi
R&D Systems
mouse monoclonal (714506)
  • ELISA; human; 3 ug/ml
In order to perform a phoshoproteomic analysis of the collagen receptor signalling network and study the importance of SHP-2 phosphorylation, R&D Systems Nck antibody (R&D Systems, 714506) was used in ELISA on human samples at 3 ug/ml. Biochem J (2013) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (15B9)
  • western blot; human; 1:1000; loading ...; fig s3c
Cell Signaling Technology Nck antibody (Cell Signaling, 2319) was used in western blot on human samples at 1:1000 (fig s3c). Nat Commun (2020) ncbi
domestic rabbit monoclonal (15B9)
  • western blot; mouse; 1:1000; fig 8
Cell Signaling Technology Nck antibody (Cell signaling, 2319) was used in western blot on mouse samples at 1:1000 (fig 8). Nat Commun (2016) ncbi
domestic rabbit monoclonal (15B9)
  • western blot; human; 1:1000; fig 4
Cell Signaling Technology Nck antibody (Cell Signaling Technologies, 2319) was used in western blot on human samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
BD Biosciences
mouse monoclonal (108/NCK)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Nck antibody (BD, 108) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (108/NCK)
  • western blot; human; 1:5000; fig 5
In order to study how Nck signaling contributes to vascular formation and remodeling, BD Biosciences Nck antibody (BD Transduction Laboratories, 610099) was used in western blot on human samples at 1:5000 (fig 5). Mol Biol Cell (2015) ncbi
Articles Reviewed
  1. Kennedy S, Jarboui M, Srihari S, Raso C, Bryan K, Dernayka L, et al. Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRASG13D. Nat Commun. 2020;11:499 pubmed publisher
  2. Gelmedin V, Morel M, Hahnel S, Cailliau K, Dissous C, Grevelding C. Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival. PLoS Pathog. 2017;13:e1006147 pubmed publisher
  3. Ishikawa E, Kosako H, Yasuda T, Ohmuraya M, Araki K, Kurosaki T, et al. Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1. Nat Commun. 2016;7:12756 pubmed publisher
  4. Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher
  5. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  6. Chaki S, Barhoumi R, Rivera G. Actin remodeling by Nck regulates endothelial lumen formation. Mol Biol Cell. 2015;26:3047-60 pubmed publisher
  7. Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, et al. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J. 2013;454:501-13 pubmed publisher