This is a Validated Antibody Database (VAD) review about human NUMA1, based on 10 published articles (read how Labome selects the articles), using NUMA1 antibody in all methods. It is aimed to help Labome visitors find the most suited NUMA1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NUMA1 synonym: NMP-22; NUMA

Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 5a
Invitrogen NUMA1 antibody (Thermo Fisher Scientific, PA5-22285) was used in immunohistochemistry on mouse samples (fig 5a). Clin Transl Med (2022) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig s2c
In order to investigate that role of an ASPM-katanin complex in microtubule and spindle pole integrity, Invitrogen NUMA1 antibody (Thermo Fisher, PA3-16829) was used in immunocytochemistry on human samples at 1:1000 (fig s2c). Nat Cell Biol (2017) ncbi
Abcam
domestic rabbit monoclonal (EP3976)
  • immunocytochemistry; human; 1:200; loading ...; fig 4d
Abcam NUMA1 antibody (Abcam, ab109262) was used in immunocytochemistry on human samples at 1:200 (fig 4d). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EP3976)
  • immunoprecipitation; human; fig 8
  • immunocytochemistry; human; 1:500; fig 4
  • western blot; human; 1:1000; fig 8
In order to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs, Abcam NUMA1 antibody (Abcam, ab109262) was used in immunoprecipitation on human samples (fig 8), in immunocytochemistry on human samples at 1:500 (fig 4) and in western blot on human samples at 1:1000 (fig 8). Nat Commun (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A73-B/D12)
  • western blot; human; 1:400; fig 4
Santa Cruz Biotechnology NUMA1 antibody (Santa Cruz, A73-B/D12) was used in western blot on human samples at 1:400 (fig 4). Cell Cycle (2015) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig s5a
In order to determine that Light Intermediate Chain 2-dynein is a major mediator of proper spindle orientation and uncover its underlying molecular mechanism, Novus Biologicals NUMA1 antibody (Novus Biologicals, NB500-174) was used in immunocytochemistry on human samples at 1:100 (fig s5a). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology NUMA1 antibody (Cell Signaling, 3888) was used in western blot on human samples (fig 1d). Sci Signal (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:50; fig 4
In order to elucidate the spindle proteome that is clathirin-dependent, Cell Signaling Technology NUMA1 antibody (Cell signaling, 3888S) was used in immunocytochemistry on human samples at 1:50 (fig 4). Mol Cell Proteomics (2016) ncbi
domestic rabbit monoclonal (D49H4)
  • western blot; human
Cell Signaling Technology NUMA1 antibody (Cell Signaling Technology, 8967) was used in western blot on human samples . Mol Oncol (2013) ncbi
BD Biosciences
mouse monoclonal (22/NuMA)
  • western blot; mouse; 1:500; fig 2a
BD Biosciences NUMA1 antibody (BD Biosciences, 610561) was used in western blot on mouse samples at 1:500 (fig 2a). elife (2021) ncbi
mouse monoclonal (22/NuMA)
  • immunoprecipitation; human; fig 5
  • western blot; human; 1:1000; fig 5
In order to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs, BD Biosciences NUMA1 antibody (BD Biosciences, 610561) was used in immunoprecipitation on human samples (fig 5) and in western blot on human samples at 1:1000 (fig 5). Nat Commun (2015) ncbi
Articles Reviewed
  1. Verreault M, Segoviano Vilchis I, Rosenberg S, Lemaire N, Schmitt C, Guehennec J, et al. Identification of growth hormone receptor as a relevant target for precision medicine in low-EGFR expressing glioblastoma. Clin Transl Med. 2022;12:e939 pubmed publisher
  2. Lau E, Damiani D, Chehade G, Ruiz Reig N, Saade R, Jossin Y, et al. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. elife. 2021;10: pubmed publisher
  3. Magescas J, Sengmanivong L, Viau A, Mayeux A, Dang T, Burtin M, et al. Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Sci Rep. 2017;7:1474 pubmed publisher
  4. Jiang K, Rezabkova L, Hua S, Liu Q, Capitani G, Altelaar A, et al. Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nat Cell Biol. 2017;19:480-492 pubmed publisher
  5. Shimizu K, Fukushima H, Ogura K, Lien E, Nihira N, Zhang J, et al. The SCF?-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis. Sci Signal. 2017;10: pubmed publisher
  6. Mahale S, Kumar M, Sharma A, Babu A, Ranjan S, Sachidanandan C, et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep. 2016;6:22 pubmed publisher
  7. Rao S, Flores Rodriguez N, Page S, Wong C, Robinson P, Chircop M. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics. 2016;15:2537-53 pubmed publisher
  8. Pillai S, Nguyen J, Johnson J, Haura E, Coppola D, Chellappan S. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun. 2015;6:10072 pubmed publisher
  9. Stolz A, Ertych N, Bastians H. A phenotypic screen identifies microtubule plus end assembly regulators that can function in mitotic spindle orientation. Cell Cycle. 2015;14:827-37 pubmed publisher
  10. Li L, Yang G, Ren C, Tanimoto R, Hirayama T, Wang J, et al. Glioma pathogenesis-related protein 1 induces prostate cancer cell death through Hsc70-mediated suppression of AURKA and TPX2. Mol Oncol. 2013;7:484-96 pubmed publisher