This is a Validated Antibody Database (VAD) review about human NR5A2, based on 7 published articles (read how Labome selects the articles), using NR5A2 antibody in all methods. It is aimed to help Labome visitors find the most suited NR5A2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NR5A2 synonym: B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F-2; nuclear receptor subfamily 5 group A member 2; CYP7A promoter-binding factor; b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus; fetoprotein-alpha 1 (AFP) transcription factor; hepatocytic transcription factor hB1F-3; liver nuclear receptor homolog-1 variant 2

R&D Systems
mouse monoclonal (H2325)
  • chromatin immunoprecipitation; mouse; fig 1
In order to investigate epigenetic changes in embryonic stem cell that control lineage commitment, R&D Systems NR5A2 antibody (R&D System, H2325) was used in chromatin immunoprecipitation on mouse samples (fig 1). Stem Cells (2015) ncbi
mouse monoclonal (H2325)
  • immunohistochemistry - paraffin section; mouse; 1:50
In order to study the effect of estradiol and androgens in severely diabetic male mice, R&D Systems NR5A2 antibody (Perseus Proteomics, PP-H2325-00) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. Endocrinology (2014) ncbi
mouse monoclonal (H2325)
  • western blot; human
R&D Systems NR5A2 antibody (R&D systems, H2325) was used in western blot on human samples . Biochem Biophys Res Commun (2013) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200
LifeSpan Biosciences NR5A2 antibody (LifeSpan Biosciences, LS-A2447) was used in immunohistochemistry - paraffin section on human samples at 1:200. Proc Natl Acad Sci U S A (2011) ncbi
EMD Millipore
mouse monoclonal (167H.4)
  • immunocytochemistry; human; fig 6
EMD Millipore NR5A2 antibody (Millipore, MAB4086) was used in immunocytochemistry on human samples (fig 6). Nat Commun (2016) ncbi
mouse monoclonal (167H.1)
  • western blot; human; 10 ug/ml
EMD Millipore NR5A2 antibody (Millipore, 167H.1) was used in western blot on human samples at 10 ug/ml. Mol Cell Biochem (2013) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunohistochemistry; human; 1:50; loading ...; fig 2d
Sigma-Aldrich NR5A2 antibody (Sigma, HPA005455) was used in immunohistochemistry on human samples at 1:50 (fig 2d). Development (2016) ncbi
Articles Reviewed
  1. Zimmerlin L, Park T, Huo J, Verma K, Pather S, Talbot C, et al. Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality. Development. 2016;143:4368-4380 pubmed
  2. Pijuan Galitó S, Tamm C, Schuster J, Sobol M, Forsberg L, Merry C, et al. Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture. Nat Commun. 2016;7:12170 pubmed publisher
  3. Kim T, Kang B, Jang H, Kwak S, Shin J, Kim H, et al. Ctbp2 Modulates NuRD-Mediated Deacetylation of H3K27 and Facilitates PRC2-Mediated H3K27me3 in Active Embryonic Stem Cell Genes During Exit from Pluripotency. Stem Cells. 2015;33:2442-55 pubmed publisher
  4. Inada A, Inada O, Fujii N, Fujishima K, Inai T, Fujii H, et al. ?-cell induction in vivo in severely diabetic male mice by changing the circulating levels and pattern of the ratios of estradiol to androgens. Endocrinology. 2014;155:3829-42 pubmed publisher
  5. Lazarus K, Zhao Z, Knower K, To S, Chand A, Clyne C. Oestradiol reduces liver receptor homolog-1 mRNA transcript stability in breast cancer cell lines. Biochem Biophys Res Commun. 2013;438:533-9 pubmed publisher
  6. Zubkova E, Semenkova L, Dudich E, Dudich I, Parfyonova Y, Menshikov M. Alpha-fetoprotein contributes to THP-1 cell invasion and chemotaxis via protein kinase and Gi-protein-dependent pathways. Mol Cell Biochem. 2013;379:283-93 pubmed publisher
  7. Benod C, Vinogradova M, Jouravel N, Kim G, Fletterick R, Sablin E. Nuclear receptor liver receptor homologue 1 (LRH-1) regulates pancreatic cancer cell growth and proliferation. Proc Natl Acad Sci U S A. 2011;108:16927-31 pubmed publisher