This is a Validated Antibody Database (VAD) review about human NPHS2, based on 19 published articles (read how Labome selects the articles), using NPHS2 antibody in all methods. It is aimed to help Labome visitors find the most suited NPHS2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NPHS2 synonym: PDCN; SRN1

Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 7g
  • western blot; mouse; 1:1000; loading ...; fig 1f
Abcam NPHS2 antibody (Abcam, ab50339) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 7g) and in western blot on mouse samples at 1:1000 (fig 1f). BMC Nephrol (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7f
Abcam NPHS2 antibody (Abcam, ab50339) was used in western blot on mouse samples at 1:1000 (fig 7f). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2b
  • western blot; human; 1:1000; loading ...; fig 4d
Abcam NPHS2 antibody (Abcam, ab50339) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 2b) and in western blot on human samples at 1:1000 (fig 4d). Int J Med Sci (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3a, 3b
  • western blot; mouse; loading ...; fig 3c, 5h
Abcam NPHS2 antibody (Abcam, ab50339) was used in immunohistochemistry - paraffin section on mouse samples (fig 3a, 3b) and in western blot on mouse samples (fig 3c, 5h). J Cell Mol Med (2019) ncbi
domestic rabbit monoclonal (EPR13820)
  • western blot; mouse; 1:500; loading ...; fig 2a
Abcam NPHS2 antibody (Abcam, Ab181143) was used in western blot on mouse samples at 1:500 (fig 2a). Kidney Blood Press Res (2018) ncbi
domestic rabbit monoclonal (EPR13820)
  • immunohistochemistry - paraffin section; mouse; 1:400; fig 4b
Abcam NPHS2 antibody (Abcam, EPR13820) was used in immunohistochemistry - paraffin section on mouse samples at 1:400 (fig 4b). Life Sci (2018) ncbi
domestic rabbit monoclonal (EPR13820)
  • western blot; mouse; 1:10,000; loading ...; fig 6a
Abcam NPHS2 antibody (Abcam, ab181143) was used in western blot on mouse samples at 1:10,000 (fig 6a). Int J Mol Med (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; fig 3
Abcam NPHS2 antibody (Abcam, ab50339) was used in immunohistochemistry - free floating section on mouse samples (fig 3). Oncogene (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2c
MilliporeSigma NPHS2 antibody (Sigma, P0372) was used in immunohistochemistry on mouse samples at 1:100 (fig 2c). Cells (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3f
MilliporeSigma NPHS2 antibody (MilliporeSigma, P0372) was used in western blot on mouse samples (fig 3f). JCI Insight (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig s4d
MilliporeSigma NPHS2 antibody (Santa, P0372) was used in immunocytochemistry on mouse samples (fig s4d). Dev Cell (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3b
MilliporeSigma NPHS2 antibody (Sigma-Aldrich, P0372) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 3b). Chin Med J (Engl) (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2b
MilliporeSigma NPHS2 antibody (Sigma-Aldrich, P0372) was used in immunohistochemistry - frozen section on mouse samples (fig 2b). J Clin Invest (2018) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4a
In order to investigate whether kidney podocytes exhibit stiffness sensitivity, MilliporeSigma NPHS2 antibody (Sigma, P0372) was used in immunocytochemistry on human samples (fig 4b) and in western blot on human samples (fig 4a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse
In order to determine the genetic factors that regulate slit diaphragm architecture, MilliporeSigma NPHS2 antibody (Sigma, P0372) was used in western blot on mouse samples . JCI Insight (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:50; tbl 1
In order to report a protocol to obtain and utilize a three-dimensional podocyte-endothelial co-culture to measure albumin permeability, MilliporeSigma NPHS2 antibody (Sigma, P0372) was used in immunocytochemistry on mouse samples at 1:50 (tbl 1). Eur J Pharm Sci (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; fig 4
In order to assess immunohistochemistry markers using four different acute and chronic rat kidney disease models, MilliporeSigma NPHS2 antibody (Sigma-Aldrich, P0372) was used in immunohistochemistry on rat samples (fig 4). Histochem Cell Biol (2016) ncbi
domestic rabbit polyclonal
MilliporeSigma NPHS2 antibody (Sigma, P0372) was used . J Cell Physiol (2015) ncbi
domestic rabbit polyclonal
MilliporeSigma NPHS2 antibody (Sigma, P0372) was used . Diabetes (2015) ncbi
Articles Reviewed
  1. Mangold N, Pippin J, Unnersjoe Jess D, Koehler S, Shankland S, Brähler S, et al. The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development. Cells. 2021;10: pubmed publisher
  2. Ren J, Xu Y, Lu X, Wang L, Ide S, Hall G, et al. Twist1 in podocytes ameliorates podocyte injury and proteinuria by limiting CCL2-dependent macrophage infiltration. JCI Insight. 2021;6: pubmed publisher
  3. Xu X, Huang X, Zhang L, Huang X, Qin Z, Hua F. Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway. BMC Nephrol. 2021;22:218 pubmed publisher
  4. Li Q, Xu Q, Tan J, Hu L, Ge C, Xu M. Carminic acid supplementation protects against fructose-induced kidney injury mainly through suppressing inflammation and oxidative stress via improving Nrf-2 signaling. Aging (Albany NY). 2021;13:10326-10353 pubmed publisher
  5. Chen Z, Zhou Q, Liu C, Zeng Y, Yuan S. Klotho deficiency aggravates diabetes-induced podocyte injury due to DNA damage caused by mitochondrial dysfunction. Int J Med Sci. 2020;17:2763-2772 pubmed publisher
  6. Huang X, Xue H, Ma J, Zhang Y, Zhang J, Liu Y, et al. Salidroside ameliorates Adriamycin nephropathy in mice by inhibiting β-catenin activity. J Cell Mol Med. 2019;23:4443-4453 pubmed publisher
  7. Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda Yamahara M, et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 2018;47:741-757.e8 pubmed publisher
  8. Hou X, Dong H, Sun L, Yang M, Cheng H, Chen Y. Purinergic 2X7 Receptor is Involved in the Podocyte Damage of Obesity-Related Glomerulopathy via Activating Nucleotide-Binding and Oligomerization Domain-Like Receptor Protein 3 Inflammasome. Chin Med J (Engl). 2018;131:2713-2725 pubmed publisher
  9. Yang X, Ding Y, Yang M, Yu L, Hu Y, Deng Y. Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5. Kidney Blood Press Res. 2018;43:616-627 pubmed publisher
  10. Qing X, Chinenov Y, Redecha P, Madaio M, Roelofs J, FARBER G, et al. iRhom2 promotes lupus nephritis through TNF-? and EGFR signaling. J Clin Invest. 2018;128:1397-1412 pubmed publisher
  11. Ma Q, Wang Y, Zhang T, Zuo W. Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy. Life Sci. 2018;193:104-109 pubmed publisher
  12. Yu J, Wu H, Liu Z, Zhu Q, Shan C, Zhang K. Advanced glycation end products induce the apoptosis of and inflammation in mouse podocytes through CXCL9-mediated JAK2/STAT3 pathway activation. Int J Mol Med. 2017;40:1185-1193 pubmed publisher
  13. Hu M, Azeloglu E, Ron A, Tran Ba K, Calizo R, Tavassoly I, et al. A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction. Sci Rep. 2017;7:43934 pubmed publisher
  14. Grahammer F, Wigge C, Schell C, Kretz O, Patrakka J, Schneider S, et al. A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes. JCI Insight. 2016;1: pubmed publisher
  15. Li M, Corbelli A, Watanabe S, Armelloni S, Ikehata M, Parazzi V, et al. Three-dimensional podocyte-endothelial cell co-cultures: Assembly, validation, and application to drug testing and intercellular signaling studies. Eur J Pharm Sci. 2016;86:1-12 pubmed publisher
  16. Funk J, Ott V, Herrmann A, Rapp W, Raab S, Riboulet W, et al. Semiautomated quantitative image analysis of glomerular immunohistochemistry markers desmin, vimentin, podocin, synaptopodin and WT-1 in acute and chronic rat kidney disease models. Histochem Cell Biol. 2016;145:315-26 pubmed publisher
  17. Sheikh B, Bechtel Walz W, Lucci J, Karpiuk O, Hild I, Hartleben B, et al. MOF maintains transcriptional programs regulating cellular stress response. Oncogene. 2016;35:2698-710 pubmed publisher
  18. Kalwa H, Storch U, Demleitner J, Fiedler S, Mayer T, Kannler M, et al. Phospholipase C epsilon (PLCε) induced TRPC6 activation: a common but redundant mechanism in primary podocytes. J Cell Physiol. 2015;230:1389-99 pubmed publisher
  19. Aggarwal P, Veron D, Thomas D, Siegel D, Moeckel G, Kashgarian M, et al. Semaphorin3a promotes advanced diabetic nephropathy. Diabetes. 2015;64:1743-59 pubmed publisher