This is a Validated Antibody Database (VAD) review about human NPC1, based on 4 published articles (read how Labome selects the articles), using NPC1 antibody in all methods. It is aimed to help Labome visitors find the most suited NPC1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NPC1 synonym: NPC; POGZ; SLC65A1

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5209)
  • western blot knockout validation; human; 1:2000; loading ...; fig 8c
Abcam NPC1 antibody (Abcam, ab134113) was used in western blot knockout validation on human samples at 1:2000 (fig 8c). elife (2019) ncbi
Abcam
domestic rabbit monoclonal (EPR5209)
  • western blot knockout validation; human; 1:2000; loading ...; fig 8c
Abcam NPC1 antibody (Abcam, ab134113) was used in western blot knockout validation on human samples at 1:2000 (fig 8c). elife (2019) ncbi
domestic rabbit monoclonal (EPR5209)
  • western blot; mouse; loading ...; fig 5a
Abcam NPC1 antibody (Abcam, ab134113) was used in western blot on mouse samples (fig 5a). Sci Rep (2017) ncbi
Invitrogen
mouse monoclonal (17B5)
  • western blot; human; 1:1000; fig 1
In order to investigate the dysfunction found in mitochondria in fibroblasts received from patients with Niemann-Pick type C disease, Invitrogen NPC1 antibody (Invitrogen, 35-1500Z) was used in western blot on human samples at 1:1000 (fig 1). Arch Biochem Biophys (2016) ncbi
Abnova
mouse monoclonal (4H2)
  • immunohistochemistry - paraffin section; human; fig 3
  • immunocytochemistry; human; fig 1
  • western blot; human; 1:1000; fig 2
Abnova NPC1 antibody (Abnova, H00004864-M02) was used in immunohistochemistry - paraffin section on human samples (fig 3), in immunocytochemistry on human samples (fig 1) and in western blot on human samples at 1:1000 (fig 2). Mol Cell Proteomics (2015) ncbi
Articles Reviewed
  1. McCauliff L, Langan A, Li R, Ilnytska O, Bose D, Waghalter M, et al. Intracellular cholesterol trafficking is dependent upon NPC2 interaction with lysobisphosphatidic acid. elife. 2019;8: pubmed publisher
  2. Vienken H, Mabrouki N, Grabau K, Claas R, Rudowski A, Schömel N, et al. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype with enhanced lysosomal Ca2+ storage. Sci Rep. 2017;7:43575 pubmed publisher
  3. Woś M, Szczepanowska J, Pikuła S, Tylki Szymańska A, Zabłocki K, Bandorowicz Pikuła J. Mitochondrial dysfunction in fibroblasts derived from patients with Niemann-Pick type C disease. Arch Biochem Biophys. 2016;593:50-9 pubmed publisher
  4. Lund R, Leth Larsen R, Caterino T, Terp M, Nissen J, Lænkholm A, et al. NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics. 2015;14:2988-99 pubmed publisher