This is a Validated Antibody Database (VAD) review about human NOTCH3, based on 33 published articles (read how Labome selects the articles), using NOTCH3 antibody in all methods. It is aimed to help Labome visitors find the most suited NOTCH3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NOTCH3 synonym: CADASIL; CADASIL1; CASIL; IMF2; LMNS

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D11B8)
  • western blot knockout validation; human; 1:1000; loading ...; fig 5c
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276s) was used in western blot knockout validation on human samples at 1:1000 (fig 5c). PLoS Genet (2021) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7f
Abcam NOTCH3 antibody (Abcam, ab23426) was used in western blot on mouse samples at 1:1000 (fig 7f). Cell Death Dis (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1c
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1c). Aging (Albany NY) (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 6f
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 6f). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 9b
Abcam NOTCH3 antibody (Abcam, ab23426) was used in western blot on human samples (fig 9b). Cancer Cell Int (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 7f
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 7f). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 4b
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on human samples (fig 4b). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s4c
Abcam NOTCH3 antibody (Abcam, ab23426) was used in western blot on human samples at 1:1000 (fig s4c). Nat Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 5n
Abcam NOTCH3 antibody (Abcam, ab23426) was used in western blot on mouse samples at 1:1000 (fig 5n). Science (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 4d
In order to report the phenotypic and genetic changes within the pre-neoplastic mammary epithelium of mice with and without stromal PTEN expression, Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on mouse samples at 1:400 (fig 4d). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2b
Abcam NOTCH3 antibody (Abcam, ab23426) was used in western blot on human samples at 1:1000 (fig 2b). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 3
Abcam NOTCH3 antibody (abcam, ab23426) was used in western blot on human samples at 1:1000 (fig 3). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:300; fig 3
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on mouse samples at 1:300 (fig 3). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; fig 6
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 6). EMBO Mol Med (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; fig 1
  • immunocytochemistry; human; 1:200; fig 2
Abcam NOTCH3 antibody (Abcam, ab23426) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1) and in immunocytochemistry on human samples at 1:200 (fig 2). PLoS ONE (2016) ncbi
Invitrogen
mouse monoclonal (MHN3-21)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, Invitrogen NOTCH3 antibody (e- Bioscienc e, 17-5787-42) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Abnova
mouse monoclonal (1G5)
  • western blot; human
Abnova NOTCH3 antibody (Abnova, H00004854-M01) was used in western blot on human samples . Cancer Res (2008) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:100; loading ...; fig 1c
Cell Signaling Technology NOTCH3 antibody (Cell Signaling Technology, 2889) was used in western blot on human samples at 1:100 (fig 1c). J Clin Invest (2022) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot knockout validation; human; 1:1000; loading ...; fig 5c
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276s) was used in western blot knockout validation on human samples at 1:1000 (fig 5c). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; mouse; 1:100; fig s4-1d
Cell Signaling Technology NOTCH3 antibody (Cell Signaling Technology, 5276) was used in western blot on mouse samples at 1:100 (fig s4-1d). elife (2020) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; loading ...; fig s1a, s1b
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276) was used in western blot on human samples (fig s1a, s1b). Mol Cancer (2019) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; 1:1000; loading ...; fig 7c, 7g
Cell Signaling Technology NOTCH3 antibody (CST, 5276s) was used in western blot on human samples at 1:1000 (fig 7c, 7g). Nat Commun (2019) ncbi
domestic rabbit monoclonal (D11B8)
  • immunocytochemistry; human; 1:100; loading ...; fig s3d
  • western blot; human; 1:1000; loading ...; fig s3c
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276) was used in immunocytochemistry on human samples at 1:100 (fig s3d) and in western blot on human samples at 1:1000 (fig s3c). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D11B8)
  • immunohistochemistry; human; 1:1000; fig 2e
  • western blot; human; 1:1000; fig 2a
Cell Signaling Technology NOTCH3 antibody (CST, 5276) was used in immunohistochemistry on human samples at 1:1000 (fig 2e) and in western blot on human samples at 1:1000 (fig 2a). J Cell Sci (2017) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; 1:1000; fig 4a
  • immunohistochemistry - paraffin section; rat; 1:800; loading ...; fig 6h
Cell Signaling Technology NOTCH3 antibody (Cell Signaling Technology, 5276) was used in western blot on human samples at 1:1000 (fig 4a) and in immunohistochemistry - paraffin section on rat samples at 1:800 (fig 6h). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D11B8)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3b
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, D11B8) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 3b). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; fig 6d
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276) was used in western blot on human samples (fig 6d). Biochem Pharmacol (2016) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; 1:1000; fig 3b
In order to show that miR-206 relieves the differentiation arrest in fusion-negative rhabdomyosarcoma, Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276) was used in western blot on human samples at 1:1000 (fig 3b). Cell Death Dis (2016) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; fig 2
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, D11B8) was used in western blot on human samples (fig 2). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 3
  • western blot; human; fig 2
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 2889) was used in western blot on mouse samples (fig 3) and in western blot on human samples (fig 2). Oncogene (2016) ncbi
domestic rabbit polyclonal
  • flow cytometry; human
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 2889) was used in flow cytometry on human samples . Stem Cells Dev (2016) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; 1:1000
In order to investigate the role of Notch signaling in human basal cell differentiation, Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human; fig 1
Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 5276) was used in western blot on human samples (fig 1). Oncotarget (2015) ncbi
domestic rabbit monoclonal (D11B8)
  • western blot; human
Cell Signaling Technology NOTCH3 antibody (Cell Signaling Technology, 5276) was used in western blot on human samples . PLoS ONE (2014) ncbi
domestic rabbit monoclonal (D11B8)
  • immunoprecipitation; human
  • western blot; human
In order to examine the effects of kinase inhibtors that target the epidermal growth factor receptor in lung cancer, Cell Signaling Technology NOTCH3 antibody (Cell Signaling, D11B8) was used in immunoprecipitation on human samples and in western blot on human samples . Cancer Res (2014) ncbi
rat monoclonal (8G5)
  • immunoprecipitation; human
  • western blot; human
In order to examine the effects of kinase inhibtors that target the epidermal growth factor receptor in lung cancer, Cell Signaling Technology NOTCH3 antibody (Cell Signaling, 8G5) was used in immunoprecipitation on human samples and in western blot on human samples . Cancer Res (2014) ncbi
MilliporeSigma
mouse monoclonal (1G5)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, MilliporeSigma NOTCH3 antibody (SIGMA, 1G5) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
Articles Reviewed
  1. Dave J, Chakraborty R, Ntokou A, Saito J, Saddouk F, Feng Z, et al. JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. J Clin Invest. 2022;132: pubmed publisher
  2. Malik N, Yan H, Yang H, Ayaz G, DuBois W, Tseng Y, et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer. PLoS Genet. 2021;17:e1009553 pubmed publisher
  3. Pan L, Lemieux M, Thomas T, Rogers J, Lipper C, Lee W, et al. IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation. elife. 2020;9: pubmed publisher
  4. Durrant C, Ruscher K, Sheppard O, Coleman M, Ozen I. Beta secretase 1-dependent amyloid precursor protein processing promotes excessive vascular sprouting through NOTCH3 signalling. Cell Death Dis. 2020;11:98 pubmed publisher
  5. Hu J, Yu J, Gan J, Song N, Shi L, Liu J, et al. Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer. Aging (Albany NY). 2020;12:2595-2609 pubmed publisher
  6. Lin Q, Chen X, Meng F, Ogawa K, Li M, Song R, et al. ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis. Mol Cancer. 2019;18:156 pubmed publisher
  7. Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  8. He W, Tang J, Li W, Li Y, Mei Y, He L, et al. Mutual regulation of JAG2 and PRAF2 promotes migration and invasion of colorectal cancer cells uncoupled from epithelial-mesenchymal transition. Cancer Cell Int. 2019;19:160 pubmed publisher
  9. Malik N, Yan H, Moshkovich N, Palangat M, Yang H, Sanchez V, et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun. 2019;10:2071 pubmed publisher
  10. Wimmer R, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505-510 pubmed publisher
  11. Mangolini M, Götte F, Moore A, Ammon T, Oelsner M, Lutzny Geier G, et al. Notch2 controls non-autonomous Wnt-signalling in chronic lymphocytic leukaemia. Nat Commun. 2018;9:3839 pubmed publisher
  12. Chakrabarti R, Celià Terrassa T, Kumar S, Hang X, Wei Y, Choudhury A, et al. Notch ligand Dll1 mediates cross-talk between mammary stem cells and the macrophageal niche. Science. 2018;360: pubmed publisher
  13. Bhardwaj A, Yang Y, Ueberheide B, Smith S. Whole proteome analysis of human tankyrase knockout cells reveals targets of tankyrase-mediated degradation. Nat Commun. 2017;8:2214 pubmed publisher
  14. Frank S, Berger P, Ljungman M, Miranti C. Human prostate luminal cell differentiation requires NOTCH3 induction by p38-MAPK and MYC. J Cell Sci. 2017;130:1952-1964 pubmed publisher
  15. Hurst L, Dunmore B, Long L, Crosby A, Al Lamki R, Deighton J, et al. TNFα drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun. 2017;8:14079 pubmed publisher
  16. Sizemore G, Balakrishnan S, Hammer A, Thies K, Trimboli A, Wallace J, et al. Stromal PTEN inhibits the expansion of mammary epithelial stem cells through Jagged-1. Oncogene. 2017;36:2297-2308 pubmed publisher
  17. Kuzmanov U, Guo H, Buchsbaum D, Cosme J, Abbasi C, Isserlin R, et al. Global phosphoproteomic profiling reveals perturbed signaling in a mouse model of dilated cardiomyopathy. Proc Natl Acad Sci U S A. 2016;113:12592-12597 pubmed
  18. Krepler C, Xiao M, Samanta M, Vultur A, Chen H, Brafford P, et al. Targeting Notch enhances the efficacy of ERK inhibitors in BRAF-V600E melanoma. Oncotarget. 2016;7:71211-71222 pubmed publisher
  19. Chen P, Qin L, Li G, Tellides G, Simons M. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation. Sci Rep. 2016;6:33407 pubmed publisher
  20. Zhang P, He D, Chen Z, Pan Q, Du F, Zang X, et al. Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer. Biochem Pharmacol. 2016;118:18-30 pubmed publisher
  21. Hanna J, Garcia M, Go J, Finkelstein D, Kodali K, Pagala V, et al. PAX7 is a required target for microRNA-206-induced differentiation of fusion-negative rhabdomyosarcoma. Cell Death Dis. 2016;7:e2256 pubmed publisher
  22. Greulich F, Rudat C, Farin H, Christoffels V, Kispert A. Lack of Genetic Interaction between Tbx18 and Tbx2/Tbx20 in Mouse Epicardial Development. PLoS ONE. 2016;11:e0156787 pubmed publisher
  23. Chen P, Qin L, Li G, Tellides G, Simons M. Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016;8:712-28 pubmed publisher
  24. Kayamori K, Katsube K, Sakamoto K, Ohyama Y, Hirai H, Yukimori A, et al. NOTCH3 Is Induced in Cancer-Associated Fibroblasts and Promotes Angiogenesis in Oral Squamous Cell Carcinoma. PLoS ONE. 2016;11:e0154112 pubmed publisher
  25. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  26. Franciosa G, Diluvio G, Gaudio F, Giuli M, Palermo R, Grazioli P, et al. Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression. Oncogene. 2016;35:4741-51 pubmed publisher
  27. Bhat V, Sun Y, Weger S, Raouf A. Notch-Induced Expression of FZD7 Requires Noncanonical NOTCH3 Signaling in Human Breast Epithelial Cells. Stem Cells Dev. 2016;25:522-9 pubmed publisher
  28. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  29. Gomi K, Arbelaez V, Crystal R, Walters M. Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway. PLoS ONE. 2015;10:e0116507 pubmed publisher
  30. Dong X, Lin Q, Aihara A, Li Y, Huang C, Chung W, et al. Aspartate β-Hydroxylase expression promotes a malignant pancreatic cellular phenotype. Oncotarget. 2015;6:1231-48 pubmed
  31. So J, Lin J, Wahler J, Liby K, Sporn M, Suh N. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS ONE. 2014;9:e107616 pubmed publisher
  32. Arasada R, Amann J, Rahman M, Huppert S, Carbone D. EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling. Cancer Res. 2014;74:5572-84 pubmed publisher
  33. Park J, Shih I, Wang T. Identification of Pbx1, a potential oncogene, as a Notch3 target gene in ovarian cancer. Cancer Res. 2008;68:8852-60 pubmed publisher