This is a Validated Antibody Database (VAD) review about human NOTCH2, based on 38 published articles (read how Labome selects the articles), using NOTCH2 antibody in all methods. It is aimed to help Labome visitors find the most suited NOTCH2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NOTCH2 synonym: AGS2; HJCYS; hN2

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D67C8)
  • immunohistochemistry knockout validation; mouse; 1:2000; loading ...; fig ed7c
  • immunohistochemistry - frozen section; mouse; 1:2000; loading ...; fig 2b
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, D67C8) was used in immunohistochemistry knockout validation on mouse samples at 1:2000 (fig ed7c) and in immunohistochemistry - frozen section on mouse samples at 1:2000 (fig 2b). Nature (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 5a
Abcam NOTCH2 antibody (Abcam, ab 8926) was used in immunohistochemistry on mouse samples (fig 5a). Biomolecules (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 9b
Abcam NOTCH2 antibody (Abcam, ab8926) was used in western blot on human samples (fig 9b). Cancer Cell Int (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:150; loading ...; fig 5b
Abcam NOTCH2 antibody (Abcam, ab8926) was used in immunohistochemistry - paraffin section on human samples at 1:150 (fig 5b). elife (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 6h
Abcam NOTCH2 antibody (Abcam, ab118824) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 6h). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 4
Abcam NOTCH2 antibody (Abcam, ab8926) was used in western blot on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
BioLegend
mouse monoclonal (MHN2-25)
  • flow cytometry; human; loading ...; fig 6
BioLegend NOTCH2 antibody (BioLegend, MHN2-25) was used in flow cytometry on human samples (fig 6). Aging Cell (2021) ncbi
Miltenyi Biotec
mouse monoclonal (MHN2-25)
  • immunocytochemistry; human; loading ...; fig s2h
Miltenyi Biotec NOTCH2 antibody (Miltenyi Biotech, 130-096-980) was used in immunocytochemistry on human samples (fig s2h). Br J Cancer (2019) ncbi
Invitrogen
rat monoclonal (16F11)
  • flow cytometry; human; tbl 1
In order to study the effect of innate lymphoid cells on B cells, Invitrogen NOTCH2 antibody (eBioscience, 16F11) was used in flow cytometry on human samples (tbl 1). Nat Immunol (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D76A6)
  • western blot; human; 1:100; loading ...
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology, 5732) was used in western blot on human samples at 1:100. J Clin Invest (2022) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; mouse; 1:100; fig s4-1d
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology, 5732) was used in western blot on mouse samples at 1:100 (fig s4-1d). elife (2020) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1c
Cell Signaling Technology NOTCH2 antibody (CST, 5732) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1c). Aging (Albany NY) (2020) ncbi
domestic rabbit monoclonal (D67C8)
  • western blot; human; loading ...; fig s1a, s1b
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 4530) was used in western blot on human samples (fig s1a, s1b). Mol Cancer (2019) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; mouse; 1:1000; loading ...; fig f1s3b
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in western blot on mouse samples at 1:1000 (fig f1s3b). elife (2019) ncbi
domestic rabbit monoclonal (D76A6)
  • immunocytochemistry; human; 1:100; fig 2g
  • western blot; human; 1:1000; loading ...; fig 2e
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in immunocytochemistry on human samples at 1:100 (fig 2g) and in western blot on human samples at 1:1000 (fig 2e). Nat Commun (2018) ncbi
domestic rabbit monoclonal (D76A6)
  • immunocytochemistry; human; loading ...; fig 7g
  • western blot; human; loading ...; fig 7c
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in immunocytochemistry on human samples (fig 7g) and in western blot on human samples (fig 7c). Cell Death Dis (2018) ncbi
domestic rabbit monoclonal (D76A6)
  • immunocytochemistry; human; 1:100; loading ...; fig 6f
  • western blot; human; 1:1000; loading ...; fig 3a
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in immunocytochemistry on human samples at 1:100 (fig 6f) and in western blot on human samples at 1:1000 (fig 3a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; mouse; 1:200; fig 5b
Cell Signaling Technology NOTCH2 antibody (CST, D76A6) was used in western blot on mouse samples at 1:200 (fig 5b). Life Sci (2018) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - paraffin section; mouse; 1:200; fig s2g
  • western blot; mouse; fig 2c
In order to study the effect of Notch signalling on small-cell lung cancer progression, Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s2g) and in western blot on mouse samples (fig 2c). Nature (2017) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; rat; fig 6a
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology, 5732) was used in western blot on rat samples (fig 6a). Sci Rep (2017) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; human; 1:1000; fig 4a
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology, 5732) was used in western blot on human samples at 1:1000 (fig 4a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - frozen section; mouse; 1:600; loading ...
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, D76A6) was used in immunohistochemistry - frozen section on mouse samples at 1:600. Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; human; fig 6d
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in western blot on human samples (fig 6d). Biochem Pharmacol (2016) ncbi
domestic rabbit monoclonal (D67C8)
  • immunohistochemistry knockout validation; mouse; 1:2000; loading ...; fig ed7c
  • immunohistochemistry - frozen section; mouse; 1:2000; loading ...; fig 2b
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, D67C8) was used in immunohistochemistry knockout validation on mouse samples at 1:2000 (fig ed7c) and in immunohistochemistry - frozen section on mouse samples at 1:2000 (fig 2b). Nature (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - frozen section; mouse; 1:400; fig 3
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in immunohistochemistry - frozen section on mouse samples at 1:400 (fig 3). Development (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - paraffin section; human; 1:100
In order to develop and characterize patient-derived primary xenografts to a model of muscle invasive bladder cancer, Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732) was used in immunohistochemistry - paraffin section on human samples at 1:100. Oncotarget (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - paraffin section; mouse; fig 2
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Tech, 5732) was used in immunohistochemistry - paraffin section on mouse samples (fig 2). Oncotarget (2015) ncbi
domestic rabbit monoclonal (D67C8)
  • immunohistochemistry - paraffin section; human
  • western blot; human; 1:1000
In order to investigate the role of Notch signaling in human basal cell differentiation, Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 4530) was used in immunohistochemistry - paraffin section on human samples and in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
  • western blot; human; fig s2
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, D76A6) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1) and in western blot on human samples (fig s2). Placenta (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • other; human; loading ...; fig 5b
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology Inc., 5732) was used in other on human samples (fig 5b) and in western blot on human samples (fig 5a). Mol Carcinog (2015) ncbi
domestic rabbit monoclonal (D67C8)
  • western blot; human; fig 1
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 4530) was used in western blot on human samples (fig 1). Oncotarget (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; human; fig 1b
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 5732S) was used in western blot on human samples (fig 1b). Leukemia (2015) ncbi
domestic rabbit monoclonal (D76A6)
  • western blot; human
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology, 5732) was used in western blot on human samples . PLoS ONE (2014) ncbi
domestic rabbit monoclonal (D67C8)
  • western blot; human
Cell Signaling Technology NOTCH2 antibody (Cell Signaling Technology, 4530P) was used in western blot on human samples . PLoS ONE (2014) ncbi
domestic rabbit monoclonal (D67C8)
  • western blot; human
Cell Signaling Technology NOTCH2 antibody (Cell Signaling, 4530) was used in western blot on human samples . Cell Cycle (2014) ncbi
Developmental Studies Hybridoma Bank
rat monoclonal (C651.6DbHN)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4i
Developmental Studies Hybridoma Bank NOTCH2 antibody (DSHB, C651.6DbHN) was used in immunohistochemistry on mouse samples at 1:200 (fig 4i). Life Sci Alliance (2021) ncbi
rat monoclonal (C651.6DbHN)
  • western blot; human; 1:1000; fig s4a
Developmental Studies Hybridoma Bank NOTCH2 antibody (Developmental studies Hybridoma Bank, C651.6DbH) was used in western blot on human samples at 1:1000 (fig s4a). Nat Commun (2018) ncbi
rat monoclonal (C651.6DbHN)
  • western blot; mouse; loading ...; fig 2e
Developmental Studies Hybridoma Bank NOTCH2 antibody (Developmental Studies Hybridoma Bank, C651.6DbHN-c) was used in western blot on mouse samples (fig 2e). Front Immunol (2018) ncbi
rat monoclonal (C651.6DbHN)
  • western blot; mouse; loading ...; fig 4a
Developmental Studies Hybridoma Bank NOTCH2 antibody (DSHB, C651.6DbHN) was used in western blot on mouse samples (fig 4a). Cell Death Dis (2016) ncbi
rat monoclonal (C651.6DbHN)
  • western blot; human; 1:1000; fig 2
  • western blot; mouse; 1:500; fig 5
Developmental Studies Hybridoma Bank NOTCH2 antibody (DSHB, C651.6DbHN) was used in western blot on human samples at 1:1000 (fig 2) and in western blot on mouse samples at 1:500 (fig 5). Oncotarget (2016) ncbi
rat monoclonal (C651.6DbHN)
  • immunohistochemistry - frozen section; mouse; 1:200
In order to show the mechanism for the effect of testosterone on mouse fetal Leydig cell progenitors, Developmental Studies Hybridoma Bank NOTCH2 antibody (Developmental Studies Hybridoma Bank, C651.6DbHN) was used in immunohistochemistry - frozen section on mouse samples at 1:200. Biol Reprod (2013) ncbi
Articles Reviewed
  1. Dave J, Chakraborty R, Ntokou A, Saito J, Saddouk F, Feng Z, et al. JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. J Clin Invest. 2022;132: pubmed publisher
  2. Racetin A, Filipović N, Lozić M, Ogata M, Gudelj Ensor L, Kelam N, et al. A Homozygous Dab1-/- Is a Potential Novel Cause of Autosomal Recessive Congenital Anomalies of the Mice Kidney and Urinary Tract. Biomolecules. 2021;11: pubmed publisher
  3. Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  4. Chung W, Challagundla L, Zhou Y, Li M, Atfi A, Xu K. Loss of Jag1 cooperates with oncogenic Kras to induce pancreatic cystic neoplasms. Life Sci Alliance. 2021;4: pubmed publisher
  5. Pan L, Lemieux M, Thomas T, Rogers J, Lipper C, Lee W, et al. IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation. elife. 2020;9: pubmed publisher
  6. Hu J, Yu J, Gan J, Song N, Shi L, Liu J, et al. Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer. Aging (Albany NY). 2020;12:2595-2609 pubmed publisher
  7. Lin Q, Chen X, Meng F, Ogawa K, Li M, Song R, et al. ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis. Mol Cancer. 2019;18:156 pubmed publisher
  8. Capulli M, Hristova D, Valbret Z, Carys K, Arjan R, Maurizi A, et al. Notch2 pathway mediates breast cancer cellular dormancy and mobilisation in bone and contributes to haematopoietic stem cell mimicry. Br J Cancer. 2019;121:157-171 pubmed publisher
  9. He W, Tang J, Li W, Li Y, Mei Y, He L, et al. Mutual regulation of JAG2 and PRAF2 promotes migration and invasion of colorectal cancer cells uncoupled from epithelial-mesenchymal transition. Cancer Cell Int. 2019;19:160 pubmed publisher
  10. Nandagopal N, Santat L, Elowitz M. Cis-activation in the Notch signaling pathway. elife. 2019;8: pubmed publisher
  11. Mangolini M, Götte F, Moore A, Ammon T, Oelsner M, Lutzny Geier G, et al. Notch2 controls non-autonomous Wnt-signalling in chronic lymphocytic leukaemia. Nat Commun. 2018;9:3839 pubmed publisher
  12. Eley L, Alqahtani A, MacGrogan D, Richardson R, Murphy L, Salguero Jimenez A, et al. A novel source of arterial valve cells linked to bicuspid aortic valve without raphe in mice. elife. 2018;7: pubmed publisher
  13. Huang G, Jiang H, Lin Y, Wu Y, Cai W, Shi B, et al. lncAKHE enhances cell growth and migration in hepatocellular carcinoma via activation of NOTCH2 signaling. Cell Death Dis. 2018;9:487 pubmed publisher
  14. Hwang I, Boularan C, Harrison K, Kehrl J. Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol. 2018;9:687 pubmed publisher
  15. Bhardwaj A, Yang Y, Ueberheide B, Smith S. Whole proteome analysis of human tankyrase knockout cells reveals targets of tankyrase-mediated degradation. Nat Commun. 2017;8:2214 pubmed publisher
  16. Ma Q, Wang Y, Zhang T, Zuo W. Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy. Life Sci. 2018;193:104-109 pubmed publisher
  17. Lim J, Ibaseta A, Fischer M, Cancilla B, O Young G, Cristea S, et al. Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer. Nature. 2017;545:360-364 pubmed publisher
  18. Rippe C, Zhu B, Krawczyk K, Bavel E, Albinsson S, Sjölund J, et al. Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Sci Rep. 2017;7:1334 pubmed publisher
  19. Hurst L, Dunmore B, Long L, Crosby A, Al Lamki R, Deighton J, et al. TNFα drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun. 2017;8:14079 pubmed publisher
  20. Kuzmanov U, Guo H, Buchsbaum D, Cosme J, Abbasi C, Isserlin R, et al. Global phosphoproteomic profiling reveals perturbed signaling in a mouse model of dilated cardiomyopathy. Proc Natl Acad Sci U S A. 2016;113:12592-12597 pubmed
  21. Zhang P, He D, Chen Z, Pan Q, Du F, Zang X, et al. Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer. Biochem Pharmacol. 2016;118:18-30 pubmed publisher
  22. Fujiwara T, Zhou J, Ye S, Zhao H. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis. 2016;7:e2300 pubmed publisher
  23. Dhar S, Kumar A, Zhang L, Rimando A, Lage J, Lewin J, et al. Dietary pterostilbene is a novel MTA1-targeted chemopreventive and therapeutic agent in prostate cancer. Oncotarget. 2016;7:18469-84 pubmed publisher
  24. Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, et al. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A. 2016;113:E71-80 pubmed publisher
  25. Pardo Saganta A, Tata P, Law B, Saez B, Chow R, Prabhu M, et al. Parent stem cells can serve as niches for their daughter cells. Nature. 2015;523:597-601 pubmed publisher
  26. Liu Z, Brunskill E, Varnum Finney B, Zhang C, Zhang A, Jay P, et al. The intracellular domains of Notch1 and Notch2 are functionally equivalent during development and carcinogenesis. Development. 2015;142:2452-63 pubmed publisher
  27. Jäger W, Xue H, Hayashi T, Janssen C, Awrey S, Wyatt A, et al. Patient-derived bladder cancer xenografts in the preclinical development of novel targeted therapies. Oncotarget. 2015;6:21522-32 pubmed
  28. Li X, Tao J, Cigliano A, Sini M, Calderaro J, Azoulay D, et al. Co-activation of PIK3CA and Yap promotes development of hepatocellular and cholangiocellular tumors in mouse and human liver. Oncotarget. 2015;6:10102-15 pubmed
  29. Gomi K, Arbelaez V, Crystal R, Walters M. Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway. PLoS ONE. 2015;10:e0116507 pubmed publisher
  30. Plessl K, Haider S, Fiala C, Pollheimer J, Knöfler M. Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast. Placenta. 2015;36:365-71 pubmed publisher
  31. Kaushik G, Venugopal A, Ramamoorthy P, Standing D, Subramaniam D, Umar S, et al. Honokiol inhibits melanoma stem cells by targeting notch signaling. Mol Carcinog. 2015;54:1710-21 pubmed publisher
  32. Dong X, Lin Q, Aihara A, Li Y, Huang C, Chung W, et al. Aspartate β-Hydroxylase expression promotes a malignant pancreatic cellular phenotype. Oncotarget. 2015;6:1231-48 pubmed
  33. Ortega M, Bhatnagar H, Lin A, Wang L, Aster J, Sill H, et al. A microRNA-mediated regulatory loop modulates NOTCH and MYC oncogenic signals in B- and T-cell malignancies. Leukemia. 2015;29:968-76 pubmed publisher
  34. So J, Lin J, Wahler J, Liby K, Sporn M, Suh N. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS ONE. 2014;9:e107616 pubmed publisher
  35. Subramani R, Lopez Valdez R, Arumugam A, Nandy S, Boopalan T, Lakshmanaswamy R. Targeting insulin-like growth factor 1 receptor inhibits pancreatic cancer growth and metastasis. PLoS ONE. 2014;9:e97016 pubmed publisher
  36. Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, et al. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol. 2014;15:354-364 pubmed publisher
  37. Singh A, Zapata M, Choi Y, Yoon S. GSI promotes vincristine-induced apoptosis by enhancing multi-polar spindle formation. Cell Cycle. 2014;13:157-66 pubmed publisher
  38. DeFalco T, Saraswathula A, Briot A, Iruela Arispe M, Capel B. Testosterone levels influence mouse fetal Leydig cell progenitors through notch signaling. Biol Reprod. 2013;88:91 pubmed publisher