This is a Validated Antibody Database (VAD) review about human NODAL, based on 4 published articles (read how Labome selects the articles), using NODAL antibody in all methods. It is aimed to help Labome visitors find the most suited NODAL antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NODAL synonym: HTX5; nodal homolog

Santa Cruz Biotechnology
mouse monoclonal (WS65)
  • immunohistochemistry; human; 1:50; loading ...; fig 1a
Santa Cruz Biotechnology NODAL antibody (Santa Cruz Biotechnology, sc-81953) was used in immunohistochemistry on human samples at 1:50 (fig 1a). Am J Cancer Res (2017) ncbi
LifeSpan Biosciences
goat polyclonal
  • immunohistochemistry; human; 1:100; fig s1
In order to research pediatric large congenital melanocytic nevi to show melanocytes affect nodal expression and signaling in melanoma cells, LifeSpan Biosciences NODAL antibody (Lifespan Biosciences, LS-B3955) was used in immunohistochemistry on human samples at 1:100 (fig s1). Int J Mol Sci (2016) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:150
  • western blot; mouse; 1:150
In order to assess the use of 3D1 mAb for treating Nodal expressing cancers, LifeSpan Biosciences NODAL antibody (LifeSpan Biosciences, LS-B3955) was used in immunohistochemistry on mouse samples at 1:150 and in western blot on mouse samples at 1:150. Oncotarget (2015) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:1000; fig s4
In order to characterize a conditional knock out of ataxia telangiectasia-mutated as a mouse model of pancreatic ductal adenocarcinoma, Abcam NODAL antibody (Abcam, 39953) was used in western blot on mouse samples at 1:1000 (fig s4). Nat Commun (2015) ncbi
Articles Reviewed
  1. Gong W, Sun B, Sun H, Zhao X, Zhang D, Liu T, et al. Nodal signaling activates the Smad2/3 pathway to regulate stem cell-like properties in breast cancer cells. Am J Cancer Res. 2017;7:503-517 pubmed
  2. Margaryan N, Gilgur A, Seftor E, Purnell C, Arva N, Gosain A, et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci. 2016;17:418 pubmed publisher
  3. Strizzi L, Sandomenico A, Margaryan N, Focà A, Sanguigno L, Bodenstine T, et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget. 2015;6:34071-86 pubmed publisher
  4. Russell R, Perkhofer L, Liebau S, Lin Q, Lechel A, Feld F, et al. Loss of ATM accelerates pancreatic cancer formation and epithelial-mesenchymal transition. Nat Commun. 2015;6:7677 pubmed publisher