This is a Validated Antibody Database (VAD) review about human NKX2 5, based on 9 published articles (read how Labome selects the articles), using NKX2 5 antibody in all methods. It is aimed to help Labome visitors find the most suited NKX2 5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NKX2 5 synonym: CHNG5; CSX; CSX1; HLHS2; NKX2.5; NKX2E; NKX4-1; VSD3

Santa Cruz Biotechnology
mouse monoclonal (A-3)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
Santa Cruz Biotechnology NKX2 5 antibody (Santa Cruz Biotechnology Inc, sc-376565) was used in immunohistochemistry - paraffin section on mouse samples (fig 5e). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (A-3)
  • western blot; mouse; fig 1b
Santa Cruz Biotechnology NKX2 5 antibody (Santa Cruz, Sc-376565) was used in western blot on mouse samples (fig 1b). J Clin Invest (2017) ncbi
mouse monoclonal (A-3)
  • immunohistochemistry; mouse; loading ...; fig 2a
  • western blot; mouse; loading ...; fig s2d
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 1c
Santa Cruz Biotechnology NKX2 5 antibody (Santa Cruz, sc-376565X) was used in immunohistochemistry on mouse samples (fig 2a), in western blot on mouse samples (fig s2d), in immunohistochemistry on human samples (fig 1a) and in western blot on human samples (fig 1c). J Am Heart Assoc (2016) ncbi
mouse monoclonal (F-2)
  • chromatin immunoprecipitation; mouse; fig 1
Santa Cruz Biotechnology NKX2 5 antibody (santa Cruz, Sc-365207) was used in chromatin immunoprecipitation on mouse samples (fig 1). elife (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2b
Abcam NKX2 5 antibody (Abcam, ab97355) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 2b). Sci Rep (2021) ncbi
domestic goat polyclonal
  • western blot; human; 1:1000; fig 4
Abcam NKX2 5 antibody (Abcam, ab106923) was used in western blot on human samples at 1:1000 (fig 4). Stem Cells Int (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • chromatin immunoprecipitation; zebrafish ; 1:100; loading ...; fig 6g
  • western blot; zebrafish ; 1:1000; loading ...; fig 7p
In order to investigate the genetic regulators of endothelial precursors in great vessel vasculogenesis, Invitrogen NKX2 5 antibody (Thermo Fisher, PA5-49431) was used in chromatin immunoprecipitation on zebrafish samples at 1:100 (fig 6g) and in western blot on zebrafish samples at 1:1000 (fig 7p). Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (E1Y8H)
  • immunohistochemistry - frozen section; human; 1:800; loading ...; fig 5f
  • flow cytometry; human; 1:1000; loading ...; fig 1b
In order to describe a protocol to generate sinoatrial node-like pacemaker cells, Cell Signaling Technology NKX2 5 antibody (Cell Signaling, 8792) was used in immunohistochemistry - frozen section on human samples at 1:800 (fig 5f) and in flow cytometry on human samples at 1:1000 (fig 1b). Nat Biotechnol (2017) ncbi
domestic rabbit monoclonal (E1Y8H)
  • western blot; domestic rabbit; 1:500; fig 3
Cell Signaling Technology NKX2 5 antibody (Cell Signaling, 8792) was used in western blot on domestic rabbit samples at 1:500 (fig 3). Mol Med Rep (2015) ncbi
Articles Reviewed
  1. Hu W, Dong A, Karasaki K, Sogabe S, Okamoto D, Saigo M, et al. Smad4 regulates the nuclear translocation of Nkx2-5 in cardiac differentiation. Sci Rep. 2021;11:3588 pubmed publisher
  2. Stopp S, Gründl M, Fackler M, Malkmus J, Leone M, Naumann R, et al. Deletion of Gas2l3 in mice leads to specific defects in cardiomyocyte cytokinesis during development. Proc Natl Acad Sci U S A. 2017;114:8029-8034 pubmed publisher
  3. Yuan X, Qi H, Li X, Wu F, Fang J, Bober E, et al. Disruption of spatiotemporal hypoxic signaling causes congenital heart disease in mice. J Clin Invest. 2017;127:2235-2248 pubmed publisher
  4. Meng Z, Liu W, Xia Y, Yin H, Zhang C, Su D, et al. The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors. Nat Commun. 2017;8:14640 pubmed publisher
  5. Du M, Wang X, Tan X, Li X, Huang D, Huang K, et al. Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice. J Am Heart Assoc. 2016;5: pubmed
  6. Protze S, Liu J, Nussinovitch U, Ohana L, Backx P, Gepstein L, et al. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker. Nat Biotechnol. 2017;35:56-68 pubmed publisher
  7. Liu H, Zhang S, Zhao L, Zhang Y, Li Q, Chai X, et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int. 2016;2016:2524092 pubmed publisher
  8. Bouveret R, Waardenberg A, Schonrock N, Ramialison M, Doan T, de Jong D, et al. NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets. elife. 2015;4: pubmed publisher
  9. Li P, Zhang L. Exogenous Nkx2.5- or GATA-4-transfected rabbit bone marrow mesenchymal stem cells and myocardial cell co-culture on the treatment of myocardial infarction in rabbits. Mol Med Rep. 2015;12:2607-21 pubmed publisher