This is a Validated Antibody Database (VAD) review about human NG2, based on 19 published articles (read how Labome selects the articles), using NG2 antibody in all methods. It is aimed to help Labome visitors find the most suited NG2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NG2 synonym: HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2

Knockout validation
Abcam
domestic rabbit monoclonal (EPR9195)
  • western blot knockout validation; human; fig 1
  • immunocytochemistry; human; 1:250; fig 2
Abcam NG2 antibody (Epitomics, 6871-1) was used in western blot knockout validation on human samples (fig 1) and in immunocytochemistry on human samples at 1:250 (fig 2). Cell Res (2015) ncbi
Invitrogen
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig 3e
Invitrogen NG2 antibody (Thermo Fisher, 37-2700) was used in immunohistochemistry - free floating section on mouse samples at 1:100 (fig 3e). elife (2020) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • flow cytometry; rat; 5 ug/ml; fig 2
In order to elucidate how CD200 restrains macrophage attacks on oligodendrocyte precursors by tollike receptor 4, Invitrogen NG2 antibody (Invitrogen, 37-2700) was used in flow cytometry on rat samples at 5 ug/ml (fig 2). J Cereb Blood Flow Metab (2016) ncbi
mouse monoclonal (N143.8)
  • flow cytometry; human; fig 1
In order to assess the preclinical safety profile of a human embryonic stem cell-derived oligodendrocyte progenitor cell therapy product to treat spinal cord injury, Invitrogen NG2 antibody (Invitrogen, 37-C2300) was used in flow cytometry on human samples (fig 1). Regen Med (2015) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry; rat; fig 4
In order to study the phenotype overlap of different glial cell populations of NG-2 (+), astroglia, and oligodendroglia cells, Invitrogen NG2 antibody (Zymed, 37-2700) was used in immunohistochemistry on rat samples (fig 4). Front Neuroanat (2015) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry - free floating section; rat; 1:300
In order to assess IL-1beta expression in periependymal astrocytes after injury, Invitrogen NG2 antibody (Invitrogen, 372700) was used in immunohistochemistry - free floating section on rat samples at 1:300. Neuroscience (2015) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry - frozen section; rat; 1:300; fig 9
In order to study the expression of Frizzled receptors in the damaged spinal cord, Invitrogen NG2 antibody (Zymed, 37-2700) was used in immunohistochemistry - frozen section on rat samples at 1:300 (fig 9). PLoS ONE (2012) ncbi
mouse monoclonal (LHM 2)
  • immunocytochemistry; human; fig 1
In order to measure the mature IgG antibody responses to melanoma from human peripheral blood B cells, Invitrogen NG2 antibody (Invitrogen, LHM2) was used in immunocytochemistry on human samples (fig 1). PLoS ONE (2011) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry; rat; fig 8e
In order to study the luminal/abluminal localization of a number of blood-brain barrier transporters expressed by endothelial cells, Invitrogen NG2 antibody (Invitrogen, N109.6) was used in immunohistochemistry on rat samples (fig 8e). Neuroscience (2008) ncbi
Abcam
domestic rabbit monoclonal (EPR20244)
  • immunohistochemistry - frozen section; mouse; 1:100; fig 5o
Abcam NG2 antibody (Abcam, ab183929) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5o). Nat Nanotechnol (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; pigs ; 1:250; loading ...; fig 2f, 2g, 2h
Abcam NG2 antibody (Abcam, ab129051) was used in immunohistochemistry - paraffin section on pigs samples at 1:250 (fig 2f, 2g, 2h). PLoS ONE (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig s9b
Abcam NG2 antibody (Abcam, ab129051) was used in immunocytochemistry on mouse samples (fig s9b). Nature (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig s1a
  • western blot; human; loading ...; fig s1b
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1e
Abcam NG2 antibody (Abcam, ab129051) was used in immunocytochemistry on human samples at 1:200 (fig s1a), in western blot on human samples (fig s1b) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 1e). J Cell Biol (2019) ncbi
domestic rabbit monoclonal (EPR9195)
  • immunohistochemistry - paraffin section; human; 1:250; loading ...; fig s10c
  • western blot; human; loading ...; fig s5h
In order to report that frizzled family proteins are receptors for Clostridium difficile toxin B, Abcam NG2 antibody (Abcam, ab139406) was used in immunohistochemistry - paraffin section on human samples at 1:250 (fig s10c) and in western blot on human samples (fig s5h). Nature (2016) ncbi
domestic rabbit monoclonal (EPR9195)
  • western blot knockout validation; human; fig 1
  • immunocytochemistry; human; 1:250; fig 2
Abcam NG2 antibody (Epitomics, 6871-1) was used in western blot knockout validation on human samples (fig 1) and in immunocytochemistry on human samples at 1:250 (fig 2). Cell Res (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (9.2.27)
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1a
Santa Cruz Biotechnology NG2 antibody (Santa Cruz, sc-80003) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1a). Transl Oncol (2022) ncbi
mouse monoclonal (G-9)
  • western blot; human; 1:500; loading ...; fig 5f
Santa Cruz Biotechnology NG2 antibody (Santa Cruz Biotechnology, sc-166251) was used in western blot on human samples at 1:500 (fig 5f). Cells (2020) ncbi
mouse monoclonal (LHM 2)
  • flow cytometry; mouse; 1:20; loading ...; fig 6c
Santa Cruz Biotechnology NG2 antibody (Santa Cruz, sc-53389) was used in flow cytometry on mouse samples at 1:20 (fig 6c). J Cell Mol Med (2014) ncbi
R&D Systems
mouse monoclonal (LHM-2)
  • mass cytometry; human; loading ...; fig 4a
R&D Systems NG2 antibody (R&D Systems, MAB2585) was used in mass cytometry on human samples (fig 4a). Cell Rep (2022) ncbi
Miltenyi Biotec
rat monoclonal (1E6.4)
  • flow cytometry; mouse; fig 2a
In order to demonstrate that type 1 T helper cells play a crucial role in vessel normalization, Miltenyi Biotec NG2 antibody (Miltenyi, 1E6.4) was used in flow cytometry on mouse samples (fig 2a). Nature (2017) ncbi
Articles Reviewed
  1. van der Heide V, Jangra S, Cohen P, Rathnasinghe R, Aslam S, Aydillo T, et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection. Cell Rep. 2022;38:110508 pubmed publisher
  2. Yang J, Liao Q, Price M, Moriarity B, Wolf N, Felices M, et al. Chondroitin sulfate proteoglycan 4, a targetable oncoantigen that promotes ovarian cancer growth, invasion, cisplatin resistance and spheroid formation. Transl Oncol. 2022;16:101318 pubmed publisher
  3. Liu Y, Wang L, Song Q, Ali M, Crowe W, Kucera G, et al. Intrapleural nano-immunotherapy promotes innate and adaptive immune responses to enhance anti-PD-L1 therapy for malignant pleural effusion. Nat Nanotechnol. 2022;17:206-216 pubmed publisher
  4. Schmitt B, Boewe A, Becker V, Nalbach L, Gu Y, Götz C, et al. Protein Kinase CK2 Regulates Nerve/Glial Antigen (NG)2-Mediated Angiogenic Activity of Human Pericytes. Cells. 2020;9: pubmed publisher
  5. Sase S, Almad A, Boecker C, Guedes Dias P, Li J, Takanohashi A, et al. TUBB4A mutations result in both glial and neuronal degeneration in an H-ABC leukodystrophy mouse model. elife. 2020;9: pubmed publisher
  6. Swier V, White K, Meyerholz D, Chefdeville A, Khanna R, Sieren J, et al. Validating indicators of CNS disorders in a swine model of neurological disease. PLoS ONE. 2020;15:e0228222 pubmed publisher
  7. Wimmer R, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505-510 pubmed publisher
  8. Niu F, Liao K, Hu G, Sil S, Callen S, Guo M, et al. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS. J Cell Biol. 2019;218:700-721 pubmed publisher
  9. Tian L, Goldstein A, Wang H, Ching Lo H, Sun Kim I, Welte T, et al. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming. Nature. 2017;544:250-254 pubmed publisher
  10. Tao L, Zhang J, Meraner P, Tovaglieri A, Wu X, Gerhard R, et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature. 2016;538:350-355 pubmed publisher
  11. Hayakawa K, Pham L, Seo J, Miyamoto N, Maki T, Terasaki Y, et al. CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation. J Cereb Blood Flow Metab. 2016;36:781-93 pubmed publisher
  12. Priest C, Manley N, Denham J, Wirth E, Lebkowski J. Preclinical safety of human embryonic stem cell-derived oligodendrocyte progenitors supporting clinical trials in spinal cord injury. Regen Med. 2015;10:939-58 pubmed publisher
  13. Alghamdi B, Fern R. Phenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+) cells. Front Neuroanat. 2015;9:49 pubmed publisher
  14. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, et al. Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 2015;25:157-68 pubmed publisher
  15. Paniagua Torija B, Arevalo Martin A, Molina Holgado E, Molina Holgado F, Garcia Ovejero D. Spinal cord injury induces a long-lasting upregulation of interleukin-1β in astrocytes around the central canal. Neuroscience. 2015;284:283-9 pubmed publisher
  16. Schuster A, Klotz M, Schwab T, Di Liddo R, Bertalot T, Schrenk S, et al. Maintenance of the enteric stem cell niche by bacterial lipopolysaccharides? Evidence and perspectives. J Cell Mol Med. 2014;18:1429-43 pubmed publisher
  17. Gonzalez P, Fernández Martos C, González Fernández C, Arenas E, Rodríguez F. Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats. PLoS ONE. 2012;7:e50793 pubmed publisher
  18. Gilbert A, Karagiannis P, Dodev T, Koers A, Lacy K, Josephs D, et al. Monitoring the systemic human memory B cell compartment of melanoma patients for anti-tumor IgG antibodies. PLoS ONE. 2011;6:e19330 pubmed publisher
  19. Roberts L, Black D, Raman C, Woodford K, Zhou M, Haggerty J, et al. Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation. Neuroscience. 2008;155:423-38 pubmed publisher