This is a Validated Antibody Database (VAD) review about human NFIX, based on 7 published articles (read how Labome selects the articles), using NFIX antibody in all methods. It is aimed to help Labome visitors find the most suited NFIX antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NFIX synonym: CTF; MRSHSS; NF-I/X; NF1-X; NF1A; SOTOS2

Knockout validation
MilliporeSigma
mouse monoclonal (3D2)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig s1b
MilliporeSigma NFIX antibody (Sigma-Aldrich, SAB1401263) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig s1b). Neural Dev (2018) ncbi
MilliporeSigma
mouse monoclonal (3D2)
  • immunohistochemistry knockout validation; mouse; 1:400; loading ...; fig 5e, h
MilliporeSigma NFIX antibody (Sigma, SAB1401263) was used in immunohistochemistry knockout validation on mouse samples at 1:400 (fig 5e, h). Development (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
Abcam NFIX antibody (Abcam, AB101341) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1c). Development (2016) ncbi
MilliporeSigma
mouse monoclonal (3D2)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig s1b
MilliporeSigma NFIX antibody (Sigma-Aldrich, SAB1401263) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig s1b). Neural Dev (2018) ncbi
mouse monoclonal (3D2)
  • immunohistochemistry; mouse; 1:2000; loading ...; tbl 1
In order to determine the expression pattern of nuclear factor I family members in the brain, MilliporeSigma NFIX antibody (Sigma, SAB1401263) was used in immunohistochemistry on mouse samples at 1:2000 (tbl 1). J Comp Neurol (2017) ncbi
mouse monoclonal (3D2)
  • immunohistochemistry knockout validation; mouse; 1:400; loading ...; fig 5e, h
MilliporeSigma NFIX antibody (Sigma, SAB1401263) was used in immunohistochemistry knockout validation on mouse samples at 1:400 (fig 5e, h). Development (2016) ncbi
mouse monoclonal (3D2)
  • immunohistochemistry; mouse; 1:200; loading ...; tbl 1
In order to report that nuclear factor one X aids in regulating progenitor cell biology within the embryonic and post-natal cerebellum and has a role within multiple neuronal and glial populations within the adult cerebellum, MilliporeSigma NFIX antibody (SIGMA, SAB1401263) was used in immunohistochemistry on mouse samples at 1:200 (tbl 1). Brain Struct Funct (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-2)
  • chromatin immunoprecipitation; human; loading ...; fig 2j
  • western blot; human; loading ...; fig 2i
Santa Cruz Biotechnology NFIX antibody (Santa Cruz, sc-74444) was used in chromatin immunoprecipitation on human samples (fig 2j) and in western blot on human samples (fig 2i). Nat Commun (2019) ncbi
mouse monoclonal (A-12)
  • EMSA; human; loading ...; fig 8a
In order to characterize pseudoexfoliation syndrome-associated genetic variants of lysyl oxidase-like 1, Santa Cruz Biotechnology NFIX antibody (Santa Cruz, sc-74445) was used in EMSA on human samples (fig 8a). Nat Commun (2017) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3g
Abcam NFIX antibody (Abcam, ab101341) was used in immunohistochemistry - frozen section on mouse samples (fig 3g). Front Neurosci (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
Abcam NFIX antibody (Abcam, AB101341) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1c). Development (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; tbl 1
In order to report that nuclear factor one X aids in regulating progenitor cell biology within the embryonic and post-natal cerebellum and has a role within multiple neuronal and glial populations within the adult cerebellum, Abcam NFIX antibody (ABCAM, AB101341) was used in immunohistochemistry on mouse samples at 1:100 (tbl 1). Brain Struct Funct (2017) ncbi
Articles Reviewed
  1. Lozzi B, Huang T, Sardar D, Huang A, Deneen B. Regionally Distinct Astrocytes Display Unique Transcription Factor Profiles in the Adult Brain. Front Neurosci. 2020;14:61 pubmed publisher
  2. Zhang J, Bai R, Li M, Ye H, Wu C, Wang C, et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun. 2019;10:1858 pubmed publisher
  3. Vidovic D, Davila R, Gronostajski R, Harvey T, Piper M. Transcriptional regulation of ependymal cell maturation within the postnatal brain. Neural Dev. 2018;13:2 pubmed publisher
  4. Pasutto F, Zenkel M, Hoja U, Berner D, Uebe S, Ferrazzi F, et al. Pseudoexfoliation syndrome-associated genetic variants affect transcription factor binding and alternative splicing of LOXL1. Nat Commun. 2017;8:15466 pubmed publisher
  5. Chen K, Harris L, Lim J, Harvey T, Piper M, Gronostajski R, et al. Differential neuronal and glial expression of nuclear factor I proteins in the cerebral cortex of adult mice. J Comp Neurol. 2017;525:2465-2483 pubmed publisher
  6. Harris L, Zalucki O, Gobius I, McDonald H, Osinki J, Harvey T, et al. Transcriptional regulation of intermediate progenitor cell generation during hippocampal development. Development. 2016;143:4620-4630 pubmed
  7. Fraser J, Essebier A, Gronostajski R, Boden M, Wainwright B, Harvey T, et al. Cell-type-specific expression of NFIX in the developing and adult cerebellum. Brain Struct Funct. 2017;222:2251-2270 pubmed publisher