This is a Validated Antibody Database (VAD) review about human NELFE, based on 3 published articles (read how Labome selects the articles), using NELFE antibody in all methods. It is aimed to help Labome visitors find the most suited NELFE antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NELFE synonym: D6S45; NELF-E; RDBP; RDP; negative elongation factor E; RD RNA-binding protein; RNA-binding protein RD; major histocompatibility complex gene RD; negative elongation factor polypeptide E; nuclear protein

Abcam
rabbit monoclonal (EPR11600)
  • western blot; human; loading ...; fig 5a
Abcam NELFE antibody (Abcam, ab170104) was used in western blot on human samples (fig 5a). FASEB J (2016) ncbi
Bethyl
rabbit polyclonal
  • immunoprecipitation; human; fig 8
Bethyl NELFE antibody (Bethyl, A301-914A) was used in immunoprecipitation on human samples (fig 8). elife (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl NELFE antibody (Bethyl, A301-913A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
Articles Reviewed
  1. Vos S, Pöllmann D, Caizzi L, Hofmann K, Rombaut P, Zimniak T, et al. Architecture and RNA binding of the human negative elongation factor. elife. 2016;5: pubmed publisher
  2. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  3. Rother S, Bartels M, Schweda A, Resch K, Pallua N, Nourbakhsh M. NF-κB-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'→3' exoribonuclease 2 and negative elongation factor. FASEB J. 2016;30:174-85 pubmed publisher