This is a Validated Antibody Database (VAD) review about human NEK2, based on 14 published articles (read how Labome selects the articles), using NEK2 antibody in all methods. It is aimed to help Labome visitors find the most suited NEK2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NEK2 synonym: HsPK21; NEK2A; NLK1; PPP1R111; RP67

Santa Cruz Biotechnology
mouse monoclonal (D-8)
  • western blot; human; 1:1000; fig 3g
Santa Cruz Biotechnology NEK2 antibody (Santa Cruz, sc-55601) was used in western blot on human samples at 1:1000 (fig 3g). Cancers (Basel) (2021) ncbi
mouse monoclonal (D-8)
  • western blot; human; fig 5a
Santa Cruz Biotechnology NEK2 antibody (SantaCruz, sc-55601) was used in western blot on human samples (fig 5a). Oncotarget (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; fig 3a
Invitrogen NEK2 antibody (Thermo Fisher, PA5-15337) was used in western blot on human samples (fig 3a). J Clin Invest (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 3e
Invitrogen NEK2 antibody (Thermo Fisher, PA5-31259) was used in immunocytochemistry on human samples (fig 3e). J Clin Invest (2017) ncbi
BD Biosciences
mouse monoclonal (20/Nek2)
  • western blot; human; 1:500; loading ...; fig 1c, 2e
BD Biosciences NEK2 antibody (BD Transduction, 610593) was used in western blot on human samples at 1:500 (fig 1c, 2e). EMBO J (2018) ncbi
mouse monoclonal (20/Nek2)
  • western blot; human; fig 1
In order to research stabilization and phosphorylation of SuFu by Nek2A as a strategy for regulating signaling of Gli2/hedgehog, BD Biosciences NEK2 antibody (BD Pharmingen, 610594) was used in western blot on human samples (fig 1). Cell Signal (2016) ncbi
mouse monoclonal (20/Nek2)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences NEK2 antibody (BD, 20) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (20/Nek2)
  • immunoprecipitation; human; fig 1
  • immunocytochemistry; human; 1:100; fig 2
  • western blot; human; 1:100; fig s11
BD Biosciences NEK2 antibody (BD Biosciences, 610593) was used in immunoprecipitation on human samples (fig 1), in immunocytochemistry on human samples at 1:100 (fig 2) and in western blot on human samples at 1:100 (fig s11). Nat Commun (2015) ncbi
mouse monoclonal (20/Nek2)
  • immunocytochemistry; human; 1:500; fig 1
  • western blot; human; 1:1000; fig 3
BD Biosciences NEK2 antibody (BD, 610594) was used in immunocytochemistry on human samples at 1:500 (fig 1) and in western blot on human samples at 1:1000 (fig 3). J Cell Biol (2015) ncbi
mouse monoclonal (20/Nek2)
  • immunoprecipitation; human; 1:500
In order to explore how Nek2 isoform A affects anaphase-promoting complex/cyclosome containing Cdc20, BD Biosciences NEK2 antibody (BD, 610593) was used in immunoprecipitation on human samples at 1:500. J Cell Sci (2015) ncbi
mouse monoclonal (20/Nek2)
  • immunocytochemistry; human
  • western blot; human
BD Biosciences NEK2 antibody (BD, #610594) was used in immunocytochemistry on human samples and in western blot on human samples . J Cell Physiol (2015) ncbi
mouse monoclonal (20/Nek2)
  • western blot; human; 1:200; fig 2
In order to report how Cep68 regulates centriole disengagement, BD Biosciences NEK2 antibody (BD Transduction Laboratories, 610593) was used in western blot on human samples at 1:200 (fig 2). Nat Cell Biol (2015) ncbi
mouse monoclonal (20/Nek2)
  • western blot; human
BD Biosciences NEK2 antibody (BD Biosciences, 610593) was used in western blot on human samples . Mol Cell Biol (2014) ncbi
mouse monoclonal (20/Nek2)
  • western blot; human; 1:1000
In order to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism, BD Biosciences NEK2 antibody (BD Transduction Laboratories, 610593) was used in western blot on human samples at 1:1000. Virology (2013) ncbi
mouse monoclonal (20/Nek2)
  • western blot; human
In order to study the role of Cdk4 and nek2 in binucleation and centrosome amplification in a her2+ breast cancer model, BD Biosciences NEK2 antibody (BD Biosciences, 610593) was used in western blot on human samples . PLoS ONE (2013) ncbi
Articles Reviewed
  1. Zhang X, Huang Z, Wang J, Ma Z, Yang J, Corey E, et al. Targeting Feedforward Loops Formed by Nuclear Receptor RORγ and Kinase PBK in mCRPC with Hyperactive AR Signaling. Cancers (Basel). 2021;13: pubmed publisher
  2. Allan L, Skowyra A, Rogers K, Zeller D, Clarke P. Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest. EMBO J. 2018;37: pubmed publisher
  3. Wang J, Cheng P, Pavlyukov M, Yu H, Zhang Z, Kim S, et al. Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2. J Clin Invest. 2017;127:3075-3089 pubmed publisher
  4. Wang Y, Li Y, Hu G, Huang X, Rao H, Xiong X, et al. Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism. Cell Signal. 2016;28:1304-13 pubmed publisher
  5. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  6. Kim S, Lee K, Choi J, Ringstad N, Dynlacht B. Nek2 activation of Kif24 ensures cilium disassembly during the cell cycle. Nat Commun. 2015;6:8087 pubmed publisher
  7. Prosser S, Sahota N, Pelletier L, Morrison C, Fry A. Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis. J Cell Biol. 2015;209:339-48 pubmed publisher
  8. Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
  9. Huang Y, Chang S, Harn H, Huang H, Lin H, Shen M, et al. Mechanosensitive store-operated calcium entry regulates the formation of cell polarity. J Cell Physiol. 2015;230:2086-97 pubmed publisher
  10. Pagan J, Marzio A, Jones M, Saraf A, Jallepalli P, Florens L, et al. Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing. Nat Cell Biol. 2015;17:31-43 pubmed publisher
  11. Yang Y, Zhou W, Xia J, Gu Z, Wendlandt E, Zhan X, et al. NEK2 mediates ALDH1A1-dependent drug resistance in multiple myeloma. Oncotarget. 2014;5:11986-97 pubmed
  12. Lee M, Moreno C, Saavedra H. E2F activators signal and maintain centrosome amplification in breast cancer cells. Mol Cell Biol. 2014;34:2581-99 pubmed
  13. Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  14. Harrison Pitner M, Saavedra H. Cdk4 and nek2 signal binucleation and centrosome amplification in a her2+ breast cancer model. PLoS ONE. 2013;8:e65971 pubmed publisher