This is a Validated Antibody Database (VAD) review about human NDUFS4, based on 11 published articles (read how Labome selects the articles), using NDUFS4 antibody in all methods. It is aimed to help Labome visitors find the most suited NDUFS4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NDUFS4 synonym: AQDQ; CI-18; CI-18 kDa; CI-AQDQ; MC1DN1

Knockout validation
Abcam
mouse monoclonal (2C7CD4AG3)
  • western blot knockout validation; mouse; fig s1
Abcam NDUFS4 antibody (Abcam, 2C7CD4AG3) was used in western blot knockout validation on mouse samples (fig s1). Mol Cell Proteomics (2016) ncbi
Abcam
mouse monoclonal (2C7CD4AG3)
  • western blot; human; fig 1b
  • western blot knockout validation; mouse; fig 1b
Abcam NDUFS4 antibody (Mitosciences, MS104) was used in western blot on human samples (fig 1b) and in western blot knockout validation on mouse samples (fig 1b). Biochim Biophys Acta (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1-E-4)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
In order to find that complex 1 -deficient Ndufs4-/- mice present with acute vision loss around p30, and this vision loss is coincident with an 'inflammatory wave', Santa Cruz Biotechnology NDUFS4 antibody (Santa Cluz, SC-100567) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a). Brain Res (2017) ncbi
Abcam
domestic rabbit monoclonal (EP7832)
  • western blot; mouse; 1:1000; loading ...; fig 4f
Abcam NDUFS4 antibody (Abcam, Ab137064) was used in western blot on mouse samples at 1:1000 (fig 4f). Acta Neuropathol Commun (2022) ncbi
domestic rabbit monoclonal (EP7832)
  • flow cytometry; human; loading ...; fig 4b
Abcam NDUFS4 antibody (Abcam, ab137064) was used in flow cytometry on human samples (fig 4b). Am J Hum Genet (2018) ncbi
domestic rabbit monoclonal (EPR7831)
  • western blot; human; loading ...; fig 4c, e2c
Abcam NDUFS4 antibody (Abcam, ab139178) was used in western blot on human samples (fig 4c, e2c). Nature (2018) ncbi
mouse monoclonal (2C7CD4AG3)
  • western blot knockout validation; mouse; fig s1
Abcam NDUFS4 antibody (Abcam, 2C7CD4AG3) was used in western blot knockout validation on mouse samples (fig s1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (2C7CD4AG3)
  • western blot; mouse; 1:1000; fig s2
Abcam NDUFS4 antibody (Abcam, ab87399) was used in western blot on mouse samples at 1:1000 (fig s2). Nat Cell Biol (2015) ncbi
mouse monoclonal (2C7CD4AG3)
  • western blot; mouse; fig 1
In order to study embryonic fibroblasts divergence from neuronal and astrocyte dysfunction in Ndufs4fky/fky mouse, Abcam NDUFS4 antibody (MitoSciences, MS104) was used in western blot on mouse samples (fig 1). Biosci Rep (2014) ncbi
mouse monoclonal (2C7CD4AG3)
  • western blot; human; fig 1b
  • western blot knockout validation; mouse; fig 1b
Abcam NDUFS4 antibody (Mitosciences, MS104) was used in western blot on human samples (fig 1b) and in western blot knockout validation on mouse samples (fig 1b). Biochim Biophys Acta (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1-E-4)
  • western blot; mouse; 1:500; loading ...; fig 6a
In order to study the effects of genetic background, natural aging, and prolonged oral administration of rotenone to mutant knock in LRRK2 mice, Santa Cruz Biotechnology NDUFS4 antibody (Santa-Cruz, sc-100567) was used in western blot on mouse samples at 1:500 (fig 6a). Sci Rep (2017) ncbi
mouse monoclonal (1-E-4)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
In order to find that complex 1 -deficient Ndufs4-/- mice present with acute vision loss around p30, and this vision loss is coincident with an 'inflammatory wave', Santa Cruz Biotechnology NDUFS4 antibody (Santa Cluz, SC-100567) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a). Brain Res (2017) ncbi
mouse monoclonal (1-E-4)
  • western blot; human
Santa Cruz Biotechnology NDUFS4 antibody (Santa Cruz, sc-100567) was used in western blot on human samples . Neurobiol Dis (2013) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; fig 4
In order to describe a signaling pathway from beta-adrenergic receptor and protein kinase A via mTORC1 that is required for adipose browning by catecholamines, Invitrogen NDUFS4 antibody (Pierce, PA5-21677) was used in western blot on mouse samples (fig 4). J Clin Invest (2016) ncbi
Articles Reviewed
  1. Creed R, Memon A, Komaragiri S, Barodia S, Goldberg M. Analysis of hemisphere-dependent effects of unilateral intrastriatal injection of α-synuclein pre-formed fibrils on mitochondrial protein levels, dynamics, and function. Acta Neuropathol Commun. 2022;10:78 pubmed publisher
  2. Piekutowska Abramczuk D, Assouline Z, Mataković L, Feichtinger R, Koňaříková E, Jurkiewicz E, et al. NDUFB8 Mutations Cause Mitochondrial Complex I Deficiency in Individuals with Leigh-like Encephalomyopathy. Am J Hum Genet. 2018;102:460-467 pubmed publisher
  3. Morscher R, Ducker G, Li S, Mayer J, Gitai Z, Sperl W, et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature. 2018;554:128-132 pubmed publisher
  4. Liu H, Ho P, Leung G, Lam C, Pang S, Li L, et al. Combined LRRK2 mutation, aging and chronic low dose oral rotenone as a model of Parkinson's disease. Sci Rep. 2017;7:40887 pubmed publisher
  5. Song L, Yu A, Murray K, Cortopassi G. Bipolar cell reduction precedes retinal ganglion neuron loss in a complex 1 knockout mouse model. Brain Res. 2017;1657:232-244 pubmed publisher
  6. Liu D, Bordicchia M, Zhang C, Fang H, Wei W, Li J, et al. Activation of mTORC1 is essential for ?-adrenergic stimulation of adipose browning. J Clin Invest. 2016;126:1704-16 pubmed publisher
  7. Piroli G, Manuel A, Clapper A, Walla M, Baatz J, Palmiter R, et al. Succination is Increased on Select Proteins in the Brainstem of the NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) Knockout Mouse, a Model of Leigh Syndrome. Mol Cell Proteomics. 2016;15:445-61 pubmed publisher
  8. Cardaci S, Zheng L, Mackay G, van den Broek N, MacKenzie E, Nixon C, et al. Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesis. Nat Cell Biol. 2015;17:1317-26 pubmed publisher
  9. Bird M, Wijeyeratne X, Komen J, Laskowski A, Ryan M, Thorburn D, et al. Neuronal and astrocyte dysfunction diverges from embryonic fibroblasts in the Ndufs4fky/fky mouse. Biosci Rep. 2014;34:e00151 pubmed publisher
  10. Schlehe J, Journel M, Taylor K, Amodeo K, LaVoie M. The mitochondrial disease associated protein Ndufaf2 is dispensable for Complex-1 assembly but critical for the regulation of oxidative stress. Neurobiol Dis. 2013;58:57-67 pubmed publisher
  11. Valsecchi F, Monge C, Forkink M, de Groof A, Benard G, Rossignol R, et al. Metabolic consequences of NDUFS4 gene deletion in immortalized mouse embryonic fibroblasts. Biochim Biophys Acta. 2012;1817:1925-36 pubmed publisher