This is a Validated Antibody Database (VAD) review about human NDC80, based on 30 published articles (read how Labome selects the articles), using NDC80 antibody in all methods. It is aimed to help Labome visitors find the most suited NDC80 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NDC80 synonym: HEC; HEC1; HsHec1; KNTC2; TID3; hsNDC80; kinetochore protein NDC80 homolog; NDC80 homolog, kinetochore complex component; NDC80 kinetochore complex component homolog; highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2; retinoblastoma-associated protein HEC

Abcam
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:300; loading ...; fig s6a
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:300 (fig s6a). J Cell Sci (2018) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:500; loading ...; fig 3e
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:500 (fig 3e). EMBO J (2018) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; loading ...; fig 6b
In order to assess the effect of three alpha-tubulin acetyltransferase 1-specific shRNAs on proliferation and morphology in HeLa and A549 cell lines, Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples (fig 6b). Cell Death Discov (2016) ncbi
mouse monoclonal (9G3)
In order to utilize expansion microscopy with fluorescent proteins and conventional antibodies, Abcam NDC80 antibody (Abcam, ab3613) was used . Nat Methods (2016) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:200; fig 2b
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:200 (fig 2b). PLoS ONE (2016) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:2000; fig s7
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:2000 (fig s7). Nat Commun (2016) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:100
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:100. elife (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; fig 1
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:2000; fig s2
Abcam NDC80 antibody (Abcam, Ab-3613) was used in immunocytochemistry on human samples at 1:2000 (fig s2). Nat Commun (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; loading ...; fig s3a
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples (fig s3a). J Cell Biol (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; loading ...; fig 4d
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples (fig 4d). Cell Cycle (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; loading ...; fig 6d
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples (fig 6d). Cell Cycle (2015) ncbi
mouse monoclonal (9G3)
  • western blot; human; 1:1000; fig 2
Abcam NDC80 antibody (Abcam, ab3613) was used in western blot on human samples at 1:1000 (fig 2). J Cell Biol (2015) ncbi
mouse monoclonal (9G3)
  • western blot; human
Abcam NDC80 antibody (Abcam, Ab3613) was used in western blot on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; fig 2
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples (fig 2). Cell Cycle (2015) ncbi
mouse monoclonal (9G3)
  • immunohistochemistry; human; 1:1000; fig 3
  • western blot; human; 1:2000; fig 2
In order to elucidate the contributions of Kid and CENP-E in chromosome congression, Abcam NDC80 antibody (Abcam, ab3613) was used in immunohistochemistry on human samples at 1:1000 (fig 3) and in western blot on human samples at 1:2000 (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:600
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:600. FEBS Lett (2014) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:1,000
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:1,000. J Cell Biol (2014) ncbi
mouse monoclonal (9G3)
  • western blot; human; 1:1000
Abcam NDC80 antibody (Abcam, ab3613) was used in western blot on human samples at 1:1000. Br J Cancer (2014) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:1000. J Biol Chem (2013) ncbi
mouse monoclonal (9G3)
  • immunocytochemistry; human; 1:50
  • immunohistochemistry; human; 1:100
  • western blot; human; 1:1000
Abcam NDC80 antibody (Abcam, ab3613) was used in immunocytochemistry on human samples at 1:50, in immunohistochemistry on human samples at 1:100 and in western blot on human samples at 1:1000. Acta Pharmacol Sin (2013) ncbi
GeneTex
mouse monoclonal (9G3.23)
  • western blot; human; loading ...; fig 1f
GeneTex NDC80 antibody (GeneTex, GTX70268) was used in western blot on human samples (fig 1f). Oncotarget (2016) ncbi
mouse monoclonal (9G3.23)
  • immunocytochemistry; human; loading ...; fig 2g
GeneTex NDC80 antibody (GeneTex, GTX70268) was used in immunocytochemistry on human samples (fig 2g). J Biol Chem (2016) ncbi
mouse monoclonal (9G3.23)
  • immunocytochemistry; human; 1:500; fig 3d
  • western blot; human; 1:1000; fig 3e
GeneTex NDC80 antibody (Genetex, 9G2.23) was used in immunocytochemistry on human samples at 1:500 (fig 3d) and in western blot on human samples at 1:1000 (fig 3e). Biol Open (2015) ncbi
mouse monoclonal (9G3.23)
  • immunocytochemistry; human
In order to study the relationship between CENP-H and kinetochore microtubules, GeneTex NDC80 antibody (GeneTex, 9G3.23) was used in immunocytochemistry on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (9G3.23)
  • western blot; Coturnix
GeneTex NDC80 antibody (GeneTex, GTX70268) was used in western blot on Coturnix samples . Curr Biol (2008) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4b
Santa Cruz Biotechnology NDC80 antibody (Santa Cruz, sc-515550) was used in immunocytochemistry on mouse samples at 1:100 (fig 4b). Cell (2019) ncbi
mouse monoclonal (1)
  • immunocytochemistry; human; 1:200; fig 2a
  • immunocytochemistry; African green monkey; 1:200; fig S2C
Santa Cruz Biotechnology NDC80 antibody (Santa Cruz, sc-135934) was used in immunocytochemistry on human samples at 1:200 (fig 2a) and in immunocytochemistry on African green monkey samples at 1:200 (fig S2C). Nat Commun (2016) ncbi
Novus Biologicals
mouse monoclonal (9G3.23)
  • immunocytochemistry; human; 1:3000; loading ...; fig 6a
Novus Biologicals NDC80 antibody (Novus Biologicals, 9G3) was used in immunocytochemistry on human samples at 1:3000 (fig 6a). J Cell Biol (2018) ncbi
Invitrogen
mouse monoclonal (9G3.23)
  • immunocytochemistry; human; 1:100
In order to study the localization of CENP-A in the cell and at centromeres, Invitrogen NDC80 antibody (Pierce, MA1-23308) was used in immunocytochemistry on human samples at 1:100. elife (2014) ncbi
Articles Reviewed
  1. Akera T, Trimm E, Lampson M. Molecular Strategies of Meiotic Cheating by Selfish Centromeres. Cell. 2019;178:1132-1144.e10 pubmed publisher
  2. Platani M, Samejima I, Samejima K, Kanemaki M, Earnshaw W. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci. 2018;131: pubmed publisher
  3. Ruppert J, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash A, et al. HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry. EMBO J. 2018;37: pubmed publisher
  4. Deluca K, Meppelink A, Broad A, Mick J, Peersen O, Pektas S, et al. Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics. J Cell Biol. 2018;217:163-177 pubmed publisher
  5. Fang Y, Kong Y, Xi J, Zhu M, Zhu T, Jiang T, et al. Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity. Oncotarget. 2016;7:79327-79341 pubmed publisher
  6. Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed publisher
  7. Chung H, Park J, Lee N, Kim H, Jang C. Phosphorylation of Astrin Regulates Its Kinetochore Function. J Biol Chem. 2016;291:17579-92 pubmed publisher
  8. Thomas G, Bandopadhyay K, Sutradhar S, Renjith M, Singh P, Gireesh K, et al. EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice. Nat Commun. 2016;7:11665 pubmed publisher
  9. Chozinski T, Halpern A, Okawa H, Kim H, Tremel G, Wong R, et al. Expansion microscopy with conventional antibodies and fluorescent proteins. Nat Methods. 2016;13:485-8 pubmed publisher
  10. Fahrenkrog B, Martinelli V, Nilles N, Fruhmann G, Chatel G, Juge S, et al. Expression of Leukemia-Associated Nup98 Fusion Proteins Generates an Aberrant Nuclear Envelope Phenotype. PLoS ONE. 2016;11:e0152321 pubmed publisher
  11. Iimori M, Watanabe S, Kiyonari S, Matsuoka K, Sakasai R, Saeki H, et al. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nat Commun. 2016;7:11117 pubmed publisher
  12. O Connor A, Maffini S, Rainey M, Kaczmarczyk A, Gaboriau D, Musacchio A, et al. Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint. Biol Open. 2015;5:11-9 pubmed publisher
  13. Zielinska A, Holubcová Z, Blayney M, Elder K, Schuh M. Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect. elife. 2015;4:e11389 pubmed publisher
  14. Ohashi A, Ohori M, Iwai K, Nambu T, Miyamoto M, Kawamoto T, et al. A Novel Time-Dependent CENP-E Inhibitor with Potent Antitumor Activity. PLoS ONE. 2015;10:e0144675 pubmed publisher
  15. Etemad B, Kuijt T, Kops G. Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Nat Commun. 2015;6:8987 pubmed publisher
  16. Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. 2015;290:27053-66 pubmed publisher
  17. Yan K, Li L, Wang X, Hong R, Zhang Y, Yang H, et al. The deubiquitinating enzyme complex BRISC is required for proper mitotic spindle assembly in mammalian cells. J Cell Biol. 2015;210:209-24 pubmed publisher
  18. Duminuco R, Noble J, Goody J, Sharma M, Ksander B, Roskelley C, et al. Integrin-linked kinase regulates senescence in an Rb-dependent manner in cancer cell lines. Cell Cycle. 2015;14:2924-37 pubmed publisher
  19. Liu C, Chuang J, Sung C, Mao Y. A dynein independent role of Tctex-1 at the kinetochore. Cell Cycle. 2015;14:1379-88 pubmed publisher
  20. Milev M, Hasaj B, Saint Dic D, Snounou S, Zhao Q, Sacher M. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol. 2015;209:221-34 pubmed publisher
  21. Zhu S, Jing R, Yang Y, Huang Y, Wang X, Leng Y, et al. A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint. J Biol Chem. 2015;290:11282-92 pubmed publisher
  22. Chen D, Ito S, Yuan H, Hyodo T, Kadomatsu K, Hamaguchi M, et al. EML4 promotes the loading of NUDC to the spindle for mitotic progression. Cell Cycle. 2015;14:1529-39 pubmed publisher
  23. Iemura K, Tanaka K. Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. Nat Commun. 2015;6:6447 pubmed publisher
  24. Silva P, Reis R, Bolanos Garcia V, Florindo C, Tavares A, Bousbaa H. Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles. FEBS Lett. 2014;588:3265-73 pubmed publisher
  25. Bodor D, Mata J, Sergeev M, David A, Salimian K, Panchenko T, et al. The quantitative architecture of centromeric chromatin. elife. 2014;3:e02137 pubmed publisher
  26. Ballister E, Riegman M, Lampson M. Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint. J Cell Biol. 2014;204:901-8 pubmed publisher
  27. Linton A, Cheng Y, Griggs K, Schedlich L, Kirschner M, Gattani S, et al. An RNAi-based screen reveals PLK1, CDK1 and NDC80 as potential therapeutic targets in malignant pleural mesothelioma. Br J Cancer. 2014;110:510-9 pubmed publisher
  28. Tan E, Caro S, Potnis A, Lanza C, Slawson C. O-linked N-acetylglucosamine cycling regulates mitotic spindle organization. J Biol Chem. 2013;288:27085-99 pubmed publisher
  29. Mo Q, Chen P, Jin X, Chen Q, Tang L, Wang B, et al. Inhibition of Hec1 expression enhances the sensitivity of human ovarian cancer cells to paclitaxel. Acta Pharmacol Sin. 2013;34:541-8 pubmed publisher
  30. Miller S, Johnson M, Stukenberg P. Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1). Curr Biol. 2008;18:1785-91 pubmed publisher