This is a Validated Antibody Database (VAD) review about human NCOA2, based on 12 published articles (read how Labome selects the articles), using NCOA2 antibody in all methods. It is aimed to help Labome visitors find the most suited NCOA2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NCOA2 synonym: GRIP1; KAT13C; NCoA-2; SRC2; TIF2; bHLHe75; nuclear receptor coactivator 2; class E basic helix-loop-helix protein 75; glucocorticoid receptor-interacting protein-1; p160 steroid receptor coactivator 2; transcriptional intermediary factor 2

Knockout validation
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; fig 4
In order to investigate Tead4-mediated transcriptional networks in various types of cancer cells, Bethyl NCOA2 antibody (Bethyl, A300-346A-T) was used in western blot knockout validation on human samples (fig 4). Cell Rep (2016) ncbi
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; 1:3000; fig 1
Abcam NCOA2 antibody (Abcam, ab10491) was used in western blot knockout validation on mouse samples at 1:3000 (fig 1). Nat Commun (2016) ncbi
Bethyl
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; mouse; fig 2
Bethyl NCOA2 antibody (Bethyl Laboratories, S300-025) was used in chromatin immunoprecipitation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig 2). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; human; fig 4
In order to investigate Tead4-mediated transcriptional networks in various types of cancer cells, Bethyl NCOA2 antibody (Bethyl, A300-346A-T) was used in western blot knockout validation on human samples (fig 4). Cell Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3
Bethyl NCOA2 antibody (Bethyl Laboratories, A300-346A) was used in western blot on mouse samples (fig 3). Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • western blot; human; fig s7
  • chromatin immunoprecipitation; mouse
Bethyl NCOA2 antibody (Bethyl, A300-346A) was used in western blot on human samples (fig s7) and in chromatin immunoprecipitation on mouse samples . J Clin Invest (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig 1
In order to study double stranded break repair during different stages of the cell cycle, Bethyl NCOA2 antibody (Bethyl, A300-345A) was used in immunocytochemistry on human samples at 1:200 (fig 1). Cell Cycle (2014) ncbi
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; 1:3000; fig 1
Abcam NCOA2 antibody (Abcam, ab10491) was used in western blot knockout validation on mouse samples at 1:3000 (fig 1). Nat Commun (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
Abcam NCOA2 antibody (Abcam, ab9261) was used in chromatin immunoprecipitation on human samples . Sci Rep (2015) ncbi
rabbit polyclonal
  • western blot; human
Abcam NCOA2 antibody (Abcam, ab10491) was used in western blot on human samples . BMC Cancer (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
Abcam NCOA2 antibody (Abcam, ab9261) was used in chromatin immunoprecipitation on human samples . BMC Cancer (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 5
Abcam NCOA2 antibody (Abcam, ab9261) was used in chromatin immunoprecipitation on human samples (fig 5). Endocrinology (2010) ncbi
BD Biosciences
mouse monoclonal (29/TIF2)
  • western blot; human; 1:2000; fig 3
BD Biosciences NCOA2 antibody (BDBiosciences, 610984) was used in western blot on human samples at 1:2000 (fig 3). AIDS Res Hum Retroviruses (2018) ncbi
mouse monoclonal (29/TIF2)
  • western blot; mouse; loading ...; fig 3a
BD Biosciences NCOA2 antibody (BD Labs, 610984) was used in western blot on mouse samples (fig 3a). J Steroid Biochem Mol Biol (2017) ncbi
mouse monoclonal (29/TIF2)
  • western blot; human; fig 1
BD Biosciences NCOA2 antibody (BD Biosciences, 610985) was used in western blot on human samples (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (29/TIF2)
  • western blot; human; 0.25 ug/ml; fig 3k
In order to discover the role of MiR137 and its effect on transciptional coregulators, BD Biosciences NCOA2 antibody (BD Transduction Laboratories, 610985) was used in western blot on human samples at 0.25 ug/ml (fig 3k). Oncotarget (2015) ncbi
mouse monoclonal (29/TIF2)
  • western blot; African green monkey
BD Biosciences NCOA2 antibody (BD Biosciences Pharmingen, 610985) was used in western blot on African green monkey samples . Biochim Biophys Acta (2015) ncbi
Articles Reviewed
  1. Saha J, Liu H, Hu P, Nikolai B, Wu H, Miao H, et al. Proteomic Profiling of a Primary CD4+ T Cell Model of HIV-1 Latency Identifies Proteins Whose Differential Expression Correlates with Reactivation of Latent HIV-1. AIDS Res Hum Retroviruses. 2018;34:103-110 pubmed publisher
  2. Patrick N, Griggs C, Icenogle A, Gilpatrick M, Kadiyala V, Jaime Frias R, et al. Class I lysine deacetylases promote glucocorticoid-induced transcriptional repression through functional interaction with LSD1. J Steroid Biochem Mol Biol. 2017;167:1-13 pubmed publisher
  3. Coppo M, Chinenov Y, Sacta M, Rogatsky I. The transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis. Nat Commun. 2016;7:12254 pubmed publisher
  4. Liu X, Li H, Rajurkar M, Li Q, Cotton J, Ou J, et al. Tead and AP1 Coordinate Transcription and Motility. Cell Rep. 2016;14:1169-1180 pubmed publisher
  5. Madsen A, Bozickovic O, Bjune J, Mellgren G, Sagen J. Metformin inhibits hepatocellular glucose, lipid and cholesterol biosynthetic pathways by transcriptionally suppressing steroid receptor coactivator 2 (SRC-2). Sci Rep. 2015;5:16430 pubmed publisher
  6. Fleet T, Zhang B, Lin F, Zhu B, Dasgupta S, Stashi E, et al. SRC-2 orchestrates polygenic inputs for fine-tuning glucose homeostasis. Proc Natl Acad Sci U S A. 2015;112:E6068-77 pubmed publisher
  7. Nilsson E, Laursen K, Whitchurch J, McWilliam A, Ødum N, Persson J, et al. MiR137 is an androgen regulated repressor of an extended network of transcriptional coregulators. Oncotarget. 2015;6:35710-25 pubmed publisher
  8. Gao L, Rabbitt E, Condon J, Renthal N, Johnston J, Mitsche M, et al. Steroid receptor coactivators 1 and 2 mediate fetal-to-maternal signaling that initiates parturition. J Clin Invest. 2015;125:2808-24 pubmed publisher
  9. Bozickovic O, Hoang T, Fenne I, Helland T, Skartveit L, Ouchida M, et al. Cyclin C interacts with steroid receptor coactivator 2 and upregulates cell cycle genes in MCF-7 cells. Biochim Biophys Acta. 2015;1853:2383-91 pubmed publisher
  10. Averbeck N, Ringel O, Herrlitz M, Jakob B, Durante M, Taucher Scholz G. DNA end resection is needed for the repair of complex lesions in G1-phase human cells. Cell Cycle. 2014;13:2509-16 pubmed publisher
  11. Wagner M, Koslowski M, Paret C, Schmidt M, Tureci O, Sahin U. NCOA3 is a selective co-activator of estrogen receptor ?-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer. 2013;13:570 pubmed publisher
  12. Vivar O, Saunier E, Leitman D, Firestone G, Bjeldanes L. Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane. Endocrinology. 2010;151:1662-7 pubmed publisher