This is a Validated Antibody Database (VAD) review about human Mad2, based on 28 published articles (read how Labome selects the articles), using Mad2 antibody in all methods. It is aimed to help Labome visitors find the most suited Mad2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Mad2 synonym: HSMAD2; MAD2

Santa Cruz Biotechnology
mouse monoclonal (C-9)
  • western blot; human; loading ...; fig 3c
Santa Cruz Biotechnology Mad2 antibody (Santa Cruz, C-9) was used in western blot on human samples (fig 3c). Cell Death Dis (2016) ncbi
mouse monoclonal (107-276-3)
  • immunocytochemistry; human; 1:75; fig 2
Santa Cruz Biotechnology Mad2 antibody (santa Cruz, sc-65492) was used in immunocytochemistry on human samples at 1:75 (fig 2). Oncotarget (2016) ncbi
mouse monoclonal (17D10)
  • western blot; human; 1:1000; loading ...; fig s5a
Santa Cruz Biotechnology Mad2 antibody (Santa Cruz, 17D10) was used in western blot on human samples at 1:1000 (fig s5a). Nat Chem Biol (2016) ncbi
mouse monoclonal (107-276-3)
  • immunocytochemistry; human; fig 2
In order to characterize the role of BAG3-HSPB8 in mitosis, Santa Cruz Biotechnology Mad2 antibody (Santa Cruz Biotechnology, sc-65492) was used in immunocytochemistry on human samples (fig 2). PLoS Genet (2015) ncbi
mouse monoclonal (17D10)
  • western blot; human; 1:1000
In order to study the interaction of human papillomaviruse E7 protein with B-Myb-MuvB complex and its effect on gene expression, Santa Cruz Biotechnology Mad2 antibody (Santa Cruz, sc-47747) was used in western blot on human samples at 1:1000. Oncogene (2014) ncbi
BioLegend
domestic rabbit polyclonal (Poly19246)
  • immunocytochemistry; human; 1:500; loading ...; fig 5d
BioLegend Mad2 antibody (Covance, PRB-452C) was used in immunocytochemistry on human samples at 1:500 (fig 5d). elife (2019) ncbi
domestic rabbit polyclonal (Poly19246)
  • immunohistochemistry; mouse; 1:300; loading ...; fig 3c
BioLegend Mad2 antibody (Biolegend, 924601) was used in immunohistochemistry on mouse samples at 1:300 (fig 3c). Nat Commun (2019) ncbi
domestic rabbit polyclonal (Poly19246)
  • immunohistochemistry; mouse; 1:300; loading ...; fig s2b
BioLegend Mad2 antibody (Biolegend, 924601) was used in immunohistochemistry on mouse samples at 1:300 (fig s2b). Curr Biol (2019) ncbi
Abcam
mouse monoclonal (17D10)
  • western blot; human; 1:500-1:1000; fig 2
Abcam Mad2 antibody (abcam, ab10691) was used in western blot on human samples at 1:500-1:1000 (fig 2). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:50; fig 5
  • western blot; human; 1:500; fig 3
Abcam Mad2 antibody (Abcam, ab97777) was used in immunocytochemistry on human samples at 1:50 (fig 5) and in western blot on human samples at 1:500 (fig 3). Nat Commun (2016) ncbi
Invitrogen
domestic rabbit polyclonal
In order to elucidate the mechanism by which BI2536 induces defects in cell proliferation of non-small cell lung cancer, Invitrogen Mad2 antibody (pierce, pa5-21594) was used . Cancer Lett (2015) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 7b
R&D Systems Mad2 antibody (Affinity, AF4005) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 7b). J Neuroinflammation (2022) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8A7)
  • western blot; mouse; loading ...; fig s3b
Cell Signaling Technology Mad2 antibody (Cell Signaling, 4636) was used in western blot on mouse samples (fig s3b). Oncogene (2017) ncbi
domestic rabbit monoclonal (D8A7)
  • western blot; human; fig 5
Cell Signaling Technology Mad2 antibody (Cell signaling, 4636) was used in western blot on human samples (fig 5). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D8A7)
  • western blot; human; loading ...; fig 4a
Cell Signaling Technology Mad2 antibody (Cell Signaling Technology, 4636) was used in western blot on human samples (fig 4a). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D8A7)
  • western blot; human
Cell Signaling Technology Mad2 antibody (Cell Signaling Technology, D8A7) was used in western blot on human samples . Nat Commun (2014) ncbi
BD Biosciences
mouse monoclonal (48/MAD2)
  • western blot; rat; 1:1000; fig 2a
BD Biosciences Mad2 antibody (BD, 610679) was used in western blot on rat samples at 1:1000 (fig 2a). Sci Adv (2021) ncbi
mouse monoclonal (48/MAD2)
  • western blot; mouse; loading ...; fig 1d
BD Biosciences Mad2 antibody (BD Biosciences, 48) was used in western blot on mouse samples (fig 1d). Cell Rep (2017) ncbi
mouse monoclonal (48/MAD2)
  • immunoprecipitation; mouse; 1:1000; fig 6
BD Biosciences Mad2 antibody (BD Transduction, 610679) was used in immunoprecipitation on mouse samples at 1:1000 (fig 6). elife (2016) ncbi
mouse monoclonal (48/MAD2)
  • immunohistochemistry; mouse; 1:2000; loading ...; fig 1c
  • western blot; mouse; 1:2000; loading ...; fig s1d
BD Biosciences Mad2 antibody (BD, 610679) was used in immunohistochemistry on mouse samples at 1:2000 (fig 1c) and in western blot on mouse samples at 1:2000 (fig s1d). Cell Rep (2016) ncbi
mouse monoclonal (48/MAD2)
  • western blot; human; fig s4b
BD Biosciences Mad2 antibody (BD Transduction Laboratorie, 610679) was used in western blot on human samples (fig s4b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (48/MAD2)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Mad2 antibody (BD, 48) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (48/MAD2)
  • western blot; human; fig 1
BD Biosciences Mad2 antibody (BD Biosciences, 610678) was used in western blot on human samples (fig 1). Cell Cycle (2015) ncbi
mouse monoclonal (48/MAD2)
  • western blot; human; fig s4
BD Biosciences Mad2 antibody (BD Biosciences, 610678) was used in western blot on human samples (fig s4). Oncogene (2015) ncbi
mouse monoclonal (48/MAD2)
  • immunoprecipitation; human; 1:500; fig 4
BD Biosciences Mad2 antibody (BD transduction laboratories, 610679) was used in immunoprecipitation on human samples at 1:500 (fig 4). Nature (2015) ncbi
mouse monoclonal (48/MAD2)
  • western blot; human
BD Biosciences Mad2 antibody (BD biosciences, 610678) was used in western blot on human samples . Cell Cycle (2014) ncbi
MBL International
monoclonal
  • western blot; human
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, MBL International Mad2 antibody (MBL, K0167-3) was used in western blot on human samples . Biol Open (2015) ncbi
monoclonal
  • immunoprecipitation; human; 1:500
In order to explore how Nek2 isoform A affects anaphase-promoting complex/cyclosome containing Cdc20, MBL International Mad2 antibody (MBL, K0167-3) was used in immunoprecipitation on human samples at 1:500. J Cell Sci (2015) ncbi
monoclonal
  • chromatin immunoprecipitation; mouse
MBL International Mad2 antibody (MBL, K0167-3) was used in chromatin immunoprecipitation on mouse samples . Cell Cycle (2014) ncbi
Articles Reviewed
  1. Ding R, Li H, Liu Y, Ou W, Zhang X, Chai H, et al. Activating cGAS-STING axis contributes to neuroinflammation in CVST mouse model and induces inflammasome activation and microglia pyroptosis. J Neuroinflammation. 2022;19:137 pubmed publisher
  2. Valussi M, Besser J, Wystub Lis K, Zukunft S, Richter M, Kubin T, et al. Repression of Osmr and Fgfr1 by miR-1/133a prevents cardiomyocyte dedifferentiation and cell cycle entry in the adult heart. Sci Adv. 2021;7:eabi6648 pubmed publisher
  3. Conti D, Gul P, Islam A, Martín Durán J, Pickersgill R, Draviam V. Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation. elife. 2019;8: pubmed publisher
  4. Paim L, FitzHarris G. Tetraploidy causes chromosomal instability in acentriolar mouse embryos. Nat Commun. 2019;10:4834 pubmed publisher
  5. Vázquez Diez C, Paim L, FitzHarris G. Cell-Size-Independent Spindle Checkpoint Failure Underlies Chromosome Segregation Error in Mouse Embryos. Curr Biol. 2019;29:865-873.e3 pubmed publisher
  6. Marks D, Thomas R, Chin Y, Shah R, Khoo C, Benezra R. Mad2 Overexpression Uncovers a Critical Role for TRIP13 in Mitotic Exit. Cell Rep. 2017;19:1832-1845 pubmed publisher
  7. Dawar S, Lim Y, Puccini J, White M, Thomas P, Bouchier Hayes L, et al. Caspase-2-mediated cell death is required for deleting aneuploid cells. Oncogene. 2017;36:2704-2714 pubmed publisher
  8. Weaver R, Limzerwala J, Naylor R, Jeganathan K, Baker D, van Deursen J. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. elife. 2016;5: pubmed publisher
  9. Zhang X, Ling Y, Guo Y, Bai Y, Shi X, Gong F, et al. Mps1 kinase regulates tumor cell viability via its novel role in mitochondria. Cell Death Dis. 2016;7:e2292 pubmed publisher
  10. Rowald K, Mantovan M, Passos J, Buccitelli C, Mardin B, Korbel J, et al. Negative Selection and Chromosome Instability Induced by Mad2 Overexpression Delay Breast Cancer but Facilitate Oncogene-Independent Outgrowth. Cell Rep. 2016;15:2679-91 pubmed publisher
  11. Chen X, Stauffer S, Chen Y, Dong J. Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. J Biol Chem. 2016;291:14761-72 pubmed publisher
  12. Tambe M, Pruikkonen S, Mäki Jouppila J, Chen P, Elgaaen B, Straume A, et al. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells' sensitivity to paclitaxel. Oncotarget. 2016;7:12267-85 pubmed publisher
  13. Mo F, Zhuang X, Liu X, Yao P, Qin B, Su Z, et al. Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Nat Chem Biol. 2016;12:226-32 pubmed publisher
  14. Craney A, Kelly A, Jia L, Fedrigo I, Yu H, Rape M. Control of APC/C-dependent ubiquitin chain elongation by reversible phosphorylation. Proc Natl Acad Sci U S A. 2016;113:1540-5 pubmed publisher
  15. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  16. Zhou H, Wang T, Zheng T, Teng J, Chen J. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 2016;7:10151 pubmed publisher
  17. Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, et al. A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis. PLoS Genet. 2015;11:e1005582 pubmed publisher
  18. Wang Z, Katsaros D, Shen Y, Fu Y, Canuto E, Benedetto C, et al. Biological and Clinical Significance of MAD2L1 and BUB1, Genes Frequently Appearing in Expression Signatures for Breast Cancer Prognosis. PLoS ONE. 2015;10:e0136246 pubmed publisher
  19. Chen D, Ito S, Yuan H, Hyodo T, Kadomatsu K, Hamaguchi M, et al. EML4 promotes the loading of NUDC to the spindle for mitotic progression. Cell Cycle. 2015;14:1529-39 pubmed publisher
  20. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  21. Kanu N, Grönroos E, Martinez P, Burrell R, Yi Goh X, Bartkova J, et al. SETD2 loss-of-function promotes renal cancer branched evolution through replication stress and impaired DNA repair. Oncogene. 2015;34:5699-708 pubmed publisher
  22. Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
  23. Choi M, Kim W, Cheon M, Lee C, Kim J. Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells. Cancer Lett. 2015;357:591-601 pubmed publisher
  24. Brownlow N, Pike T, Zicha D, Collinson L, Parker P. Mitotic catenation is monitored and resolved by a PKCε-regulated pathway. Nat Commun. 2014;5:5685 pubmed publisher
  25. Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  26. d Alcontres M, Palacios J, Mejias D, Blasco M. TopoII? prevents telomere fragility and formation of ultra thin DNA bridges during mitosis through TRF1-dependent binding to telomeres. Cell Cycle. 2014;13:1463-81 pubmed publisher
  27. Singh A, Zapata M, Choi Y, Yoon S. GSI promotes vincristine-induced apoptosis by enhancing multi-polar spindle formation. Cell Cycle. 2014;13:157-66 pubmed publisher
  28. Pang C, Toh S, He P, Teissier S, Ben Khalifa Y, Xue Y, et al. A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c). Oncogene. 2014;33:4039-49 pubmed publisher