This is a Validated Antibody Database (VAD) review about human MYLK, based on 17 published articles (read how Labome selects the articles), using MYLK antibody in all methods. It is aimed to help Labome visitors find the most suited MYLK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MYLK synonym: AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK; myosin light chain kinase, smooth muscle; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; telokin

Knockout validation
Sigma-Aldrich
mouse monoclonal (K36)
  • western blot knockout validation; mouse; 1:10,000; fig s3a
Sigma-Aldrich MYLK antibody (Sigma, K36) was used in western blot knockout validation on mouse samples at 1:10,000 (fig s3a). Nat Commun (2016) ncbi
Abcam
rabbit monoclonal (EP1458Y)
  • western blot; mouse; fig s12d
Abcam MYLK antibody (Abcam, ab76092) was used in western blot on mouse samples (fig s12d). Science (2018) ncbi
rabbit monoclonal (EP1458Y)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 9h
Abcam MYLK antibody (Abcam, ab76092) was used in immunohistochemistry - frozen section on mouse samples (fig 9h). Dev Biol (2017) ncbi
rabbit monoclonal (EP1458Y)
  • western blot; rat; fig 4g
Abcam MYLK antibody (Abcam, ab76092) was used in western blot on rat samples (fig 4g). Sci Rep (2017) ncbi
rabbit monoclonal (EP1458Y)
  • western blot; rat; loading ...; fig 7
Abcam MYLK antibody (Abcam, ab76092) was used in western blot on rat samples (fig 7). PLoS ONE (2017) ncbi
rabbit monoclonal (EP1458Y)
  • western blot; human; loading ...; fig 3d
In order to examine phosphorylated proteins during spontaneous and oxytocin-driven phasic contractions, Abcam MYLK antibody (Abcam, ab76092) was used in western blot on human samples (fig 3d). Biochem Biophys Res Commun (2017) ncbi
rabbit monoclonal (EP1458Y)
  • western blot; mouse; loading ...; fig s7a
In order to elucidate the role of IP3R in regulating blood pressure in vivo, Abcam MYLK antibody (Abcam, ab76092) was used in western blot on mouse samples (fig s7a). JCI Insight (2016) ncbi
rabbit monoclonal (EP1458Y)
  • western blot; mouse; loading ...; fig 4c
In order to discover that calcium-calmodulin-dependent kinase II is an important regulator of smooth muscle function in angiotensin-II hypertension, Abcam MYLK antibody (Abcam, ab76092) was used in western blot on mouse samples (fig 4c). Vascul Pharmacol (2016) ncbi
rabbit monoclonal (EP1458Y)
  • immunocytochemistry; rat; 1:250
Abcam MYLK antibody (Abcam, ab76092) was used in immunocytochemistry on rat samples at 1:250. Cell Tissue Res (2013) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; fig 4
In order to study inhibition of Rac1 trafficking to the cell border by RhoB control of the endothelial barrier recovery, Invitrogen MYLK antibody (Invitrogen, 44-260G) was used in western blot on human samples (fig 4). J Cell Biol (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; rat; fig 8
  • immunocytochemistry; rat
  • western blot; rat; fig 3
In order to study the involvement of nuclear nonmuscle myosin regulatory light chain in response to myocardial ischemia/reperfusion, Invitrogen MYLK antibody (Invitrogen, 44-260 G) was used in chromatin immunoprecipitation on rat samples (fig 8), in immunocytochemistry on rat samples and in western blot on rat samples (fig 3). Free Radic Biol Med (2015) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • western blot; cow; loading ...; fig 4 - s2a
In order to investigate the mechanosensitivity of mesenchymal stem cell, LifeSpan Biosciences MYLK antibody (LSBio, LS-C25729) was used in western blot on cow samples (fig 4 - s2a). elife (2016) ncbi
Sigma-Aldrich
mouse monoclonal (K36)
  • western blot; mouse; fig 2i
Sigma-Aldrich MYLK antibody (Sigma-Aldrich, M7905) was used in western blot on mouse samples (fig 2i). Cell (2018) ncbi
mouse monoclonal (K36)
  • western blot knockout validation; mouse; 1:10,000; fig s3a
Sigma-Aldrich MYLK antibody (Sigma, K36) was used in western blot knockout validation on mouse samples at 1:10,000 (fig s3a). Nat Commun (2016) ncbi
mouse monoclonal (K36)
  • western blot; human; fig 1
Sigma-Aldrich MYLK antibody (Sigma-Aldrich, M7905) was used in western blot on human samples (fig 1). Oncogene (2016) ncbi
mouse monoclonal (K36)
  • western blot; human; 1:1000
In order to study the efficacy of (-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity, Sigma-Aldrich MYLK antibody (Sigma, M7905) was used in western blot on human samples at 1:1000. Arch Biochem Biophys (2015) ncbi
mouse monoclonal (K36)
  • western blot; mouse
Sigma-Aldrich MYLK antibody (Sigma, K36) was used in western blot on mouse samples . Int J Biochem Cell Biol (2014) ncbi
mouse monoclonal (K36)
  • western blot; rat; 1:5000
In order to study the role of TEAD proteins in regulating expression of other genes in vascular smooth muscle cells, Sigma-Aldrich MYLK antibody (Sigma, M7905) was used in western blot on rat samples at 1:5000. J Biol Chem (2014) ncbi
Articles Reviewed
  1. Albrengues J, Shields M, Ng D, Park C, Ambrico A, Poindexter M, et al. Neutrophil extracellular traps produced during inflammation awaken dormant cancer cells in mice. Science. 2018;361: pubmed publisher
  2. Silva C, Peyre E, Adhikari M, Tielens S, Tanco S, Van Damme P, et al. Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell. 2018;172:1063-1078.e19 pubmed publisher
  3. Logan C, Rajakaruna S, Bowen C, Radice G, Robinson M, Menko A. N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis. Dev Biol. 2017;428:118-134 pubmed publisher
  4. Rippe C, Zhu B, Krawczyk K, Bavel E, Albinsson S, Sjölund J, et al. Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Sci Rep. 2017;7:1334 pubmed publisher
  5. Zhai L, Liu M, Wang T, Zhang H, Li S, Guo Y. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS ONE. 2017;12:e0174414 pubmed publisher
  6. Hudson C, Lopez Bernal A. Phosphorylation of proteins during human myometrial contractions: A phosphoproteomic approach. Biochem Biophys Res Commun. 2017;482:1393-1399 pubmed publisher
  7. Heo S, Driscoll T, Thorpe S, Nerurkar N, Baker B, Yang M, et al. Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity. elife. 2016;5: pubmed publisher
  8. Lin Q, Zhao G, Fang X, Peng X, Tang H, Wang H, et al. IP3 receptors regulate vascular smooth muscle contractility and hypertension. JCI Insight. 2016;1:e89402 pubmed
  9. Prasad A, Ketsawatsomkron P, Nuno D, Koval O, Dibbern M, Venema A, et al. Role of CaMKII in Ang-II-dependent small artery remodeling. Vascul Pharmacol. 2016;87:172-179 pubmed publisher
  10. Marcos Ramiro B, García Weber D, Barroso S, Feito J, Ortega M, Cernuda Morollón E, et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. J Cell Biol. 2016;213:385-402 pubmed publisher
  11. Zhang C, Wang P, Liu D, Chen C, Zhao W, Chen X, et al. The molecular basis of the genesis of basal tone in internal anal sphincter. Nat Commun. 2016;7:11358 pubmed publisher
  12. Kim D, Helfman D. Loss of MLCK leads to disruption of cell-cell adhesion and invasive behavior of breast epithelial cells via increased expression of EGFR and ERK/JNK signaling. Oncogene. 2016;35:4495-508 pubmed publisher
  13. Contreras T, Ricciardi E, Cremonini E, Oteiza P. (-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity. Arch Biochem Biophys. 2015;573:84-91 pubmed publisher
  14. Zhang Y, Liu B, Luo X, Zhang J, Li N, Ma Q, et al. A novel function of nuclear nonmuscle myosin regulatory light chain in promotion of xanthine oxidase transcription after myocardial ischemia/reperfusion. Free Radic Biol Med. 2015;83:115-28 pubmed publisher
  15. Wang Y, Zhao W, Zhang L, Zhao Y, Li F, Zhang Z, et al. Molecular and cellular basis of the regulation of lymphatic contractility and lymphatic absorption. Int J Biochem Cell Biol. 2014;53:134-40 pubmed publisher
  16. Liu F, Wang X, Hu G, Wang Y, Zhou J. The transcription factor TEAD1 represses smooth muscle-specific gene expression by abolishing myocardin function. J Biol Chem. 2014;289:3308-16 pubmed publisher
  17. Huggins C, Povstyan O, Harhun M. Characterization of transcriptional and posttranscriptional properties of native and cultured phenotypically modulated vascular smooth muscle cells. Cell Tissue Res. 2013;352:265-75 pubmed publisher