This is a Validated Antibody Database (VAD) review about human MYH4, based on 19 published articles (read how Labome selects the articles), using MYH4 antibody in all methods. It is aimed to help Labome visitors find the most suited MYH4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MYH4 synonym: MYH2B; MyHC-2B; MyHC-IIb

Santa Cruz Biotechnology
mouse monoclonal (B-5)
  • western blot; mouse; loading ...; fig 9
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, sc-376157) was used in western blot on mouse samples (fig 9). Physiol Rep (2021) ncbi
mouse monoclonal (F59)
  • immunocytochemistry; human; loading ...; fig 2c
  • western blot; human; 1:300; loading ...; fig 2c
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz Biotechnology, Sc-32732) was used in immunocytochemistry on human samples (fig 2c) and in western blot on human samples at 1:300 (fig 2c). elife (2020) ncbi
mouse monoclonal (B-5)
  • western blot; mouse; 1:2000; loading ...; fig 1c
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, sc-376157) was used in western blot on mouse samples at 1:2000 (fig 1c). Aging (Albany NY) (2020) ncbi
mouse monoclonal (F59)
  • immunohistochemistry; giant danio ; 2 ug/ml; loading ...; fig 4i
Santa Cruz Biotechnology MYH4 antibody (Santa, sc?\32732) was used in immunohistochemistry on giant danio samples at 2 ug/ml (fig 4i). Dev Dyn (2019) ncbi
mouse monoclonal (B-5)
  • immunocytochemistry; human; 1:100; loading ...
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz Biotechnology, sc-376157) was used in immunocytochemistry on human samples at 1:100. elife (2019) ncbi
mouse monoclonal (B-5)
  • immunocytochemistry; mouse; 1:2000; loading ...; fig 2c
  • western blot; mouse; 1:2000; loading ...; fig 2b
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, sc-376157) was used in immunocytochemistry on mouse samples at 1:2000 (fig 2c) and in western blot on mouse samples at 1:2000 (fig 2b). Gene (2017) ncbi
mouse monoclonal (B-5)
  • western blot; mouse; 1:1000; fig 4d
Santa Cruz Biotechnology MYH4 antibody (SantaCruz, sc-376157) was used in western blot on mouse samples at 1:1000 (fig 4d). Sci Rep (2017) ncbi
mouse monoclonal (B-5)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 5b ii
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, sc-376157) was used in immunocytochemistry on mouse samples at 1:100 (fig 5b ii). Biomater Res (2017) ncbi
mouse monoclonal (B-5)
  • western blot; mouse; loading ...; fig 1c
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, sc-376157) was used in western blot on mouse samples (fig 1c). Oncotarget (2016) ncbi
mouse monoclonal (F59)
  • western blot; mouse; fig 5
In order to analyze promotion of development of distinct sarcoma subtypes in hepatocyte growth factor-mediated satellite cells niche disruption, Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, sc-32732) was used in western blot on mouse samples (fig 5). elife (2016) ncbi
mouse monoclonal (F59)
  • western blot; human
Santa Cruz Biotechnology MYH4 antibody (Santa Cruz, SC-32732) was used in western blot on human samples . PLoS ONE (2015) ncbi
Invitrogen
mouse monoclonal (MF20)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 6i
  • western blot; mouse; loading ...; fig 6b
Invitrogen MYH4 antibody (Invitrogen, 53-6503-82) was used in immunocytochemistry on mouse samples at 1:100 (fig 6i) and in western blot on mouse samples (fig 6b). Cell Death Dis (2021) ncbi
mouse monoclonal (MF20)
  • flow cytometry; mouse; 1:100; loading ...; fig 3b
Invitrogen MYH4 antibody (eBioscience, 53-6503-82) was used in flow cytometry on mouse samples at 1:100 (fig 3b). Mol Med Rep (2018) ncbi
mouse monoclonal (MYSN02 (MY-32))
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 6c
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4b
In order to study the role of myomerger in skeletal muscle development, Invitrogen MYH4 antibody (ThermoFisher, MA5-11748) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 6c) and in immunocytochemistry on mouse samples at 1:100 (fig 4b). Nat Commun (2017) ncbi
mouse monoclonal (MF20)
  • immunohistochemistry - frozen section; zebrafish ; 1:75; loading ...; fig s2c
In order to examine cardiac revascularization in zebrafish, Invitrogen MYH4 antibody (eBiosciences, MF20) was used in immunohistochemistry - frozen section on zebrafish samples at 1:75 (fig s2c). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (MY32)
  • immunohistochemistry - frozen section; mouse; fig 1
In order to test if the "dying back" axonopathy in a pure fast-fatigable alpha-motor axon nerve is a length-dependent process, Invitrogen MYH4 antibody (Thermo Fisher, My32) was used in immunohistochemistry - frozen section on mouse samples (fig 1). Neuroscience (2016) ncbi
mouse monoclonal (MF20)
  • flow cytometry; mouse
Invitrogen MYH4 antibody (Affymetrix eBioscience, 53-6503-82) was used in flow cytometry on mouse samples . Circ Cardiovasc Genet (2014) ncbi
mouse monoclonal (MF20)
  • immunocytochemistry; mouse; 1:200
In order to analyze the conversion increase of fibroblasts to induced cardiomyocytes through inhibition of TGFbeta signaling, Invitrogen MYH4 antibody (eBioscience, 53-6503-82) was used in immunocytochemistry on mouse samples at 1:200. PLoS ONE (2014) ncbi
mouse monoclonal (MF20)
  • immunocytochemistry; mouse; 1:200
In order to evaluate the method for measuring fibroblast reprogramming to cardiomyocytes, Invitrogen MYH4 antibody (eBioscience, 53-6503-82) was used in immunocytochemistry on mouse samples at 1:200. J Mol Cell Cardiol (2013) ncbi
Articles Reviewed
  1. Al Zaeed N, Budai Z, Szondy Z, Sarang Z. TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice. Cell Death Dis. 2021;12:611 pubmed publisher
  2. Azar C, Valentine M, Trausch Azar J, Rois L, Mahjoub M, Nelson D, et al. RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts. Physiol Rep. 2021;9:e14671 pubmed publisher
  3. Pal A, Leung J, Ang G, Rao V, Pignata L, Lim H, et al. EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma. elife. 2020;9: pubmed publisher
  4. Chung L, Liu S, Huang S, Salter D, Lee H, Hsu Y. High phosphate induces skeletal muscle atrophy and suppresses myogenic differentiation by increasing oxidative stress and activating Nrf2 signaling. Aging (Albany NY). 2020;12:21446-21468 pubmed publisher
  5. Nelson H, Coffing G, Chilson S, Hester K, Carrillo C, Ostreicher S, et al. Structure, development, and functional morphology of the cement gland of the giant danio, Devario malabaricus. Dev Dyn. 2019;248:1155-1174 pubmed publisher
  6. Herdy J, Schäfer S, Kim Y, Ansari Z, Zangwill D, Ku M, et al. Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. elife. 2019;8: pubmed publisher
  7. Tian J, Wang R, Hou Q, Li M, Chen L, Deng X, et al. Optimization and enrichment of induced cardiomyocytes derived from mouse fibroblasts by reprogramming with cardiac transcription factors. Mol Med Rep. 2018;17:3912-3920 pubmed publisher
  8. Wang X, Zeng R, Xu H, Xu Z, Zuo B. The nuclear protein-coding gene ANKRD23 negatively regulates myoblast differentiation. Gene. 2017;629:68-75 pubmed publisher
  9. Guo Y, Wang J, Zhu M, Zeng R, Xu Z, Li G, et al. Identification of MyoD-Responsive Transcripts Reveals a Novel Long Non-coding RNA (lncRNA-AK143003) that Negatively Regulates Myoblast Differentiation. Sci Rep. 2017;7:2828 pubmed publisher
  10. Quinn M, Goh Q, Kurosaka M, Gamage D, Petrany M, Prasad V, et al. Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development. Nat Commun. 2017;8:15665 pubmed publisher
  11. Cha S, Lee H, Koh W. Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns. Biomater Res. 2017;21:1 pubmed publisher
  12. Marín Juez R, Marass M, Gauvrit S, Rossi A, Lai S, Materna S, et al. Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proc Natl Acad Sci U S A. 2016;113:11237-11242 pubmed
  13. Ramazzotti G, Billi A, Manzoli L, Mazzetti C, Ruggeri A, Erneux C, et al. IPMK and β-catenin mediate PLC-β1-dependent signaling in myogenic differentiation. Oncotarget. 2016;7:84118-84127 pubmed publisher
  14. Morena D, Maestro N, Bersani F, Forni P, Lingua M, Foglizzo V, et al. Hepatocyte Growth Factor-mediated satellite cells niche perturbation promotes development of distinct sarcoma subtypes. elife. 2016;5: pubmed publisher
  15. Tallon C, Russell K, Sakhalkar S, Andrapallayal N, Farah M. Length-dependent axo-terminal degeneration at the neuromuscular synapses of type II muscle in SOD1 mice. Neuroscience. 2016;312:179-89 pubmed publisher
  16. Faggi F, Codenotti S, Poliani P, Cominelli M, Chiarelli N, Colombi M, et al. MURC/cavin-4 Is Co-Expressed with Caveolin-3 in Rhabdomyosarcoma Tumors and Its Silencing Prevents Myogenic Differentiation in the Human Embryonal RD Cell Line. PLoS ONE. 2015;10:e0130287 pubmed publisher
  17. Martinez Fernandez A, Nelson T, Reyes S, Alekseev A, Secreto F, Perez Terzic C, et al. iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes. Circ Cardiovasc Genet. 2014;7:667-76 pubmed publisher
  18. Ifkovits J, Addis R, Epstein J, Gearhart J. Inhibition of TGF? signaling increases direct conversion of fibroblasts to induced cardiomyocytes. PLoS ONE. 2014;9:e89678 pubmed publisher
  19. Addis R, Ifkovits J, Pinto F, Kellam L, Esteso P, Rentschler S, et al. Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. J Mol Cell Cardiol. 2013;60:97-106 pubmed publisher