This is a Validated Antibody Database (VAD) review about human MYCN, based on 38 published articles (read how Labome selects the articles), using MYCN antibody in all methods. It is aimed to help Labome visitors find the most suited MYCN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MYCN synonym: MODED; N-myc; NMYC; ODED; bHLHe37

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (B8.4.B)
  • western blot knockout validation; mouse; fig 5c
Santa Cruz Biotechnology MYCN antibody (SantaCruz, sc-53993) was used in western blot knockout validation on mouse samples (fig 5c). Nat Commun (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B8.4.B)
  • western blot; mouse; 1:1000; loading ...; fig 5b
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in western blot on mouse samples at 1:1000 (fig 5b). Front Cell Dev Biol (2021) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; loading ...; fig 3b, s4a
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-56729) was used in western blot on human samples (fig 3b, s4a). Neoplasia (2021) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; 1:200; fig s9g
  • ChIP-Seq; mouse; loading ...; fig 6a
  • western blot; mouse; 1:200; loading ...; fig s2e
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in western blot on human samples at 1:200 (fig s9g), in ChIP-Seq on mouse samples (fig 6a) and in western blot on mouse samples at 1:200 (fig s2e). Nat Commun (2021) ncbi
mouse monoclonal (B8.4.B)
  • flow cytometry; human; loading ...; fig 4b
Santa Cruz Biotechnology MYCN antibody (Santa Cruz Biotechnology, sc-53993) was used in flow cytometry on human samples (fig 4b). elife (2020) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; 1:1000; loading ...; fig 2a
Santa Cruz Biotechnology MYCN antibody (Santa Cruz Biotechnology, sc-53993) was used in western blot on human samples at 1:1000 (fig 2a). Nat Commun (2019) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; 1:200; loading ...; fig 4e, 4h
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in western blot on human samples at 1:200 (fig 4e, 4h). Stem Cells (2019) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; 1:500; loading ...; fig 2c
Santa Cruz Biotechnology MYCN antibody (Santa, sc-53993) was used in western blot on human samples at 1:500 (fig 2c). Biochem Pharmacol (2019) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology MYCN antibody (SantaCruz, sc-56729) was used in western blot on human samples (fig 3a). Nucleic Acids Res (2018) ncbi
mouse monoclonal (B8.4.B)
  • western blot knockout validation; mouse; fig 5c
Santa Cruz Biotechnology MYCN antibody (SantaCruz, sc-53993) was used in western blot knockout validation on mouse samples (fig 5c). Nat Commun (2018) ncbi
mouse monoclonal (B8.4.B)
  • western blot; mouse; 1:1000; loading ...; fig 5b
In order to study the role of microRNAs in the cell cycle and differentiation, Santa Cruz Biotechnology MYCN antibody (Santa Cruz Biotechnology, sc-53993) was used in western blot on mouse samples at 1:1000 (fig 5b). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (B8.4.B)
  • immunohistochemistry - paraffin section; human; 1:500; fig s9a
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig s9a). Nat Genet (2017) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; 1:200; loading ...; fig 3d
In order to elucidate the contribution of MDM2 expression to retinoblastoma tumorigenesis, Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-56729) was used in western blot on human samples at 1:200 (fig 3d). Oncogene (2017) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; 1:2000; loading ...; fig 1a
Santa Cruz Biotechnology MYCN antibody (SantaCruz, sc-53993) was used in western blot on human samples at 1:2000 (fig 1a). Oncotarget (2016) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; loading ...; fig s2b
Santa Cruz Biotechnology MYCN antibody (Cell Signaling, sc-53993) was used in western blot on human samples (fig s2b). Nature (2016) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; 1:750; loading ...; fig 1
In order to present the role of bromodomain and hedgehog pathway on small cell lung cancer, Santa Cruz Biotechnology MYCN antibody (Santa Cruz Biotechnology, 56729) was used in western blot on human samples at 1:750 (fig 1). Cancer Lett (2016) ncbi
mouse monoclonal (B8.4.B)
  • ChIP-Seq; human; fig 5
  • western blot; human; 1:1000; fig s3
Santa Cruz Biotechnology MYCN antibody (santa Cruz, sc-53993) was used in ChIP-Seq on human samples (fig 5) and in western blot on human samples at 1:1000 (fig s3). Oncotarget (2015) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; fig 1
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc53993) was used in western blot on human samples (fig 1). Oncotarget (2015) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; fig s1c
  • western blot; mouse; fig 5a
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, SC53993) was used in western blot on human samples (fig s1c) and in western blot on mouse samples (fig 5a). Cell Death Differ (2016) ncbi
mouse monoclonal (B8.4.B)
  • chromatin immunoprecipitation; human; fig 3A
  • immunoprecipitation; human; fig 1A
  • western blot; human; fig 1A
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in chromatin immunoprecipitation on human samples (fig 3A), in immunoprecipitation on human samples (fig 1A) and in western blot on human samples (fig 1A). Oncotarget (2015) ncbi
mouse monoclonal (B8.4.B)
  • western blot; human; 1:500; fig s1
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in western blot on human samples at 1:500 (fig s1). Oncotarget (2015) ncbi
mouse monoclonal (B8.4.B)
In order to study the relationship between SKP2 and MYCN, Santa Cruz Biotechnology MYCN antibody (Santa-Cruz, SC-53993) was used . Cancer Lett (2015) ncbi
mouse monoclonal (B8.4.B)
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, B8.4.B) was used . Mutat Res (2015) ncbi
mouse monoclonal (B8.4.B)
  • chromatin immunoprecipitation; human
  • western blot; human; fig 6
In order to study the genes in the MYCN gene network in relation to nueroblastic cancerous cells, Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used in chromatin immunoprecipitation on human samples and in western blot on human samples (fig 6). J Biol Chem (2015) ncbi
mouse monoclonal (B8.4.B)
  • immunoprecipitation; human; fig 8
In order to identify and characterize MYCN protein inhibitors, Santa Cruz Biotechnology MYCN antibody (Santa Cruz, SC-53993) was used in immunoprecipitation on human samples (fig 8). Cancer Cell (2014) ncbi
mouse monoclonal (B8.4.B)
In order to investigate the role of asymmetric cell division during tumorigenesis, Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-53993) was used . Cancer Res (2014) ncbi
mouse monoclonal (2)
  • western blot; human
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-142) was used in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (B8.4.B)
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
Santa Cruz Biotechnology MYCN antibody (Santa Cruz Biotechnology, sc-53993) was used in chromatin immunoprecipitation on human samples and in immunoprecipitation on human samples . J Biol Chem (2013) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human
Santa Cruz Biotechnology MYCN antibody (Santa Cruz, sc-56729) was used in western blot on human samples . EMBO J (2012) ncbi
Abcam
mouse monoclonal (NCM II 100)
  • western blot; human; loading ...; fig 3b, s4a
Abcam MYCN antibody (Abcam, ab16898) was used in western blot on human samples (fig 3b, s4a). Neoplasia (2021) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; 1:250; loading ...; fig 5d
Abcam MYCN antibody (Abcam, ab16898) was used in western blot on human samples at 1:250 (fig 5d). Nat Commun (2020) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; 1:1000
Abcam MYCN antibody (Abcam, ab16898) was used in western blot on human samples at 1:1000. Cancer Cell Int (2013) ncbi
mouse monoclonal (NCM II 100)
  • western blot; human; 1:250
Abcam MYCN antibody (Abcam, ab16898) was used in western blot on human samples at 1:250. Cancer Res (2013) ncbi
mouse monoclonal (NCM II 100)
  • immunohistochemistry - frozen section; chicken; 1:10
Abcam MYCN antibody (Abcam, ab16898) was used in immunohistochemistry - frozen section on chicken samples at 1:10. Oncogenesis (2012) ncbi
mouse monoclonal (NCM II 100)
  • western blot; mouse
Abcam MYCN antibody (Abcam, ab16898) was used in western blot on mouse samples . Dev Biol (2013) ncbi
Invitrogen
domestic rabbit polyclonal
In order to study the role of Orai1 in amelogenesis, Invitrogen MYCN antibody (Thermo Fisher Scientific, PA5-17403) was used . Dev Dyn (2015) ncbi
domestic rabbit polyclonal
In order to investigate the effect of nifurtimox on tumor metabolism, Invitrogen MYCN antibody (Thermo Fisher Scientific, PA5-17403) was used . Cancer Biol Ther (2015) ncbi
Novus Biologicals
mouse monoclonal (NMYC-1)
Novus Biologicals MYCN antibody (Novus Biologicals, NB200-109) was used . Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology MYCN antibody (Cell Signaling, 9405) was used in western blot on human samples at 1:1000 (fig 3b). Cancer Res (2018) ncbi
domestic rabbit polyclonal
Cell Signaling Technology MYCN antibody (Cell signaling, 9405) was used . Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 5a
  • chromatin immunoprecipitation; human; loading ...; fig 4d
  • western blot; human; loading ...; fig 4a
Cell Signaling Technology MYCN antibody (Cell Signaling, 9405) was used in immunohistochemistry - paraffin section on human samples (fig 5a), in chromatin immunoprecipitation on human samples (fig 4d) and in western blot on human samples (fig 4a). J Mol Med (Berl) (2016) ncbi
Articles Reviewed
  1. Dong F, Chen M, Jiang L, Shen Z, Ma L, Han C, et al. PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Plzf Expression via Modulation of Lysine Histone Modifications. Front Cell Dev Biol. 2021;9:673258 pubmed publisher
  2. Yi J, Sias Garcia O, Nasholm N, Hu X, Iniguez A, Hall M, et al. The synergy of BET inhibitors with aurora A kinase inhibitors in MYCN-amplified neuroblastoma is heightened with functional TP53. Neoplasia. 2021;23:624-633 pubmed publisher
  3. Brady N, Bagadion A, Singh R, Conteduca V, Van Emmenis L, Arceci E, et al. Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer. Nat Commun. 2021;12:3372 pubmed publisher
  4. Kuchen E, Becker N, Claudino N, Hofer T. Hidden long-range memories of growth and cycle speed correlate cell cycles in lineage trees. elife. 2020;9: pubmed publisher
  5. Almstedt E, Elgendy R, Hekmati N, Rosén E, Wärn C, Olsen T, et al. Integrative discovery of treatments for high-risk neuroblastoma. Nat Commun. 2020;11:71 pubmed publisher
  6. Liu P, Tee A, Milazzo G, Hannan K, Maag J, Mondal S, et al. The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35. Nat Commun. 2019;10:5026 pubmed publisher
  7. Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett P, et al. Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells. Stem Cells. 2019;37:1293-1306 pubmed publisher
  8. Mooney M, Geerts D, Kort E, Bachmann A. Anti-tumor effect of sulfasalazine in neuroblastoma. Biochem Pharmacol. 2019;162:237-249 pubmed publisher
  9. Lambert M, Terrone S, Giraud G, Benoit Pilven C, Cluet D, Combaret V, et al. The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. Nucleic Acids Res. 2018;46:7686-7700 pubmed publisher
  10. Mirzamohammadi F, Kozlova A, Papaioannou G, Paltrinieri E, Ayturk U, Kobayashi T. Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models. Nat Commun. 2018;9:1352 pubmed publisher
  11. Jung Y, Cackowski F, Yumoto K, Decker A, Wang J, Kim J, et al. CXCL12γ Promotes Metastatic Castration-Resistant Prostate Cancer by Inducing Cancer Stem Cell and Neuroendocrine Phenotypes. Cancer Res. 2018;78:2026-2039 pubmed publisher
  12. Otto T, Candido S, Pilarz M, Sicinska E, Bronson R, Bowden M, et al. Cell cycle-targeting microRNAs promote differentiation by enforcing cell-cycle exit. Proc Natl Acad Sci U S A. 2017;114:10660-10665 pubmed publisher
  13. van Groningen T, Koster J, Valentijn L, Zwijnenburg D, Akogul N, Hasselt N, et al. Neuroblastoma is composed of two super-enhancer-associated differentiation states. Nat Genet. 2017;49:1261-1266 pubmed publisher
  14. Qi D, Cobrinik D. MDM2 but not MDM4 promotes retinoblastoma cell proliferation through p53-independent regulation of MYCN translation. Oncogene. 2017;36:1760-1769 pubmed publisher
  15. Murakami Tonami Y, Ikeda H, Yamagishi R, Inayoshi M, Inagaki S, Kishida S, et al. SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci Rep. 2016;6:31615 pubmed publisher
  16. Xue C, Yu D, Gherardi S, Koach J, Milazzo G, Gamble L, et al. MYCN promotes neuroblastoma malignancy by establishing a regulatory circuit with transcription factor AP4. Oncotarget. 2016;7:54937-54951 pubmed publisher
  17. Powers J, Tsanov K, Pearson D, Roels F, Spina C, EBRIGHT R, et al. Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma. Nature. 2016;535:246-51 pubmed publisher
  18. Zheng G, Li N, Jia X, Peng C, Luo L, Deng Y, et al. MYCN-mediated miR-21 overexpression enhances chemo-resistance via targeting CADM1 in tongue cancer. J Mol Med (Berl). 2016;94:1129-1141 pubmed
  19. Kaur G, Reinhart R, Monks A, Evans D, Morris J, Polley E, et al. Bromodomain and hedgehog pathway targets in small cell lung cancer. Cancer Lett. 2016;371:225-39 pubmed publisher
  20. Duffy D, Krstic A, Halasz M, Schwarzl T, Fey D, Iljin K, et al. Integrative omics reveals MYCN as a global suppressor of cellular signalling and enables network-based therapeutic target discovery in neuroblastoma. Oncotarget. 2015;6:43182-201 pubmed publisher
  21. Xiao D, Ren P, Su H, Yue M, Xiu R, Hu Y, et al. Myc promotes glutaminolysis in human neuroblastoma through direct activation of glutaminase 2. Oncotarget. 2015;6:40655-66 pubmed publisher
  22. Zheng L, Zinn V, Lefkelidou A, Taqi N, Chatzistavrou X, Balam T, et al. Orai1 expression pattern in tooth and craniofacial ectodermal tissues and potential functions during ameloblast differentiation. Dev Dyn. 2015;244:1249-58 pubmed publisher
  23. Cabanillas Stanchi K, Bruchelt G, Handgretinger R, Holzer U. Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma. Cancer Biol Ther. 2015;16:1353-63 pubmed publisher
  24. Petroni M, Sardina F, Heil C, Sahún Roncero M, Colicchia V, Veschi V, et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ. 2016;23:197-206 pubmed publisher
  25. Amente S, Milazzo G, Sorrentino M, Ambrosio S, Di Palo G, Lania L, et al. Lysine-specific demethylase (LSD1/KDM1A) and MYCN cooperatively repress tumor suppressor genes in neuroblastoma. Oncotarget. 2015;6:14572-83 pubmed
  26. Chen L, Rousseau R, Middleton S, Nichols G, Newell D, Lunec J, et al. Pre-clinical evaluation of the MDM2-p53 antagonist RG7388 alone and in combination with chemotherapy in neuroblastoma. Oncotarget. 2015;6:10207-21 pubmed
  27. Evans L, Chen L, Milazzo G, Gherardi S, Perini G, Willmore E, et al. SKP2 is a direct transcriptional target of MYCN and a potential therapeutic target in neuroblastoma. Cancer Lett. 2015;363:37-45 pubmed publisher
  28. Ambrosio S, Amente S, Napolitano G, Di Palo G, Lania L, Majello B. MYC impairs resolution of site-specific DNA double-strand breaks repair. Mutat Res. 2015;774:6-13 pubmed publisher
  29. Chayka O, D Acunto C, Middleton O, Arab M, Sala A. Identification and pharmacological inactivation of the MYCN gene network as a therapeutic strategy for neuroblastic tumor cells. J Biol Chem. 2015;290:2198-212 pubmed publisher
  30. Gustafson W, Meyerowitz J, Nekritz E, Chen J, Benes C, Charron E, et al. Drugging MYCN through an allosteric transition in Aurora kinase A. Cancer Cell. 2014;26:414-427 pubmed publisher
  31. Izumi H, Kaneko Y. Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res. 2014;74:5620-30 pubmed publisher
  32. Sabò A, Doni M, Amati B. SUMOylation of Myc-family proteins. PLoS ONE. 2014;9:e91072 pubmed publisher
  33. Zhu Q, Feng C, Liao W, Zhang Y, Tang S. Target delivery of MYCN siRNA by folate-nanoliposomes delivery system in a metastatic neuroblastoma model. Cancer Cell Int. 2013;13:65 pubmed publisher
  34. Murray M, Saini H, Siegler C, Hanning J, Barker E, van Dongen S, et al. LIN28 Expression in malignant germ cell tumors downregulates let-7 and increases oncogene levels. Cancer Res. 2013;73:4872-84 pubmed publisher
  35. Carter R, Mullassery D, See V, Theocharatos S, Pizer B, Losty P, et al. Exploitation of chick embryo environments to reprogram MYCN-amplified neuroblastoma cells to a benign phenotype, lacking detectable MYCN expression. Oncogenesis. 2012;1:e24 pubmed publisher
  36. Corvetta D, Chayka O, Gherardi S, D Acunto C, Cantilena S, Valli E, et al. Physical interaction between MYCN oncogene and polycomb repressive complex 2 (PRC2) in neuroblastoma: functional and therapeutic implications. J Biol Chem. 2013;288:8332-41 pubmed publisher
  37. Morlando M, Dini Modigliani S, Torrelli G, Rosa A, Di Carlo V, Caffarelli E, et al. FUS stimulates microRNA biogenesis by facilitating co-transcriptional Drosha recruitment. EMBO J. 2012;31:4502-10 pubmed publisher
  38. Harmelink C, Peng Y, Debenedittis P, Chen H, Shou W, Jiao K. Myocardial Mycn is essential for mouse ventricular wall morphogenesis. Dev Biol. 2013;373:53-63 pubmed publisher