This is a Validated Antibody Database (VAD) review about human MTDH, based on 14 published articles (read how Labome selects the articles), using MTDH antibody in all methods. It is aimed to help Labome visitors find the most suited MTDH antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MTDH synonym: 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3; protein LYRIC; 3D3/LYRIC; astrocyte elevated gene 1; astrocyte elevated gene-1 protein; lysine-rich CEACAM1 co-isolated protein; metastasis adhesion protein

Invitrogen
rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 3g
  • immunoprecipitation; human; loading ...; fig 2b
  • western blot; human; 1:1000; loading ...; fig 1b
In order to discuss how c-jun regulates astrocyte elevated gene-1 in gliomas, Invitrogen MTDH antibody (Thermo Fisher Scientific, 40-6500) was used in chromatin immunoprecipitation on human samples (fig 3g), in immunoprecipitation on human samples (fig 2b) and in western blot on human samples at 1:1000 (fig 1b). Mol Cell Biol (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig 1
In order to determine the role of miR-506 in osteosarcoma pathogenesis, Invitrogen MTDH antibody (Invitrogen, 40-6500) was used in western blot on human samples at 1:500 (fig 1). Oncol Lett (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4a
In order to assess the power of MiR-630 in supressing breast cancer through metadherin, Invitrogen MTDH antibody (Invitrogen, 40-6400) was used in western blot on human samples (fig 4a). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; mouse
In order to examine how metadherin contributes to drug resistance, metastasis, and angiogenesis, Invitrogen MTDH antibody (Invitrogen, 40-6500) was used in western blot on mouse samples . J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:250
In order to investigate metadherin expression, significance, and related mechanism in chronic lymphocytic leukemia, Invitrogen MTDH antibody (Invitrogen, 40-6500) was used in western blot on human samples at 1:250. Med Oncol (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 20 ug/ml; fig 4
  • western blot; human; 1 ug/ml; fig 2
In order to study cadmium-breast cancer cell interactions, Invitrogen MTDH antibody (Invitrogen, 40-6500) was used in immunocytochemistry on human samples at 20 ug/ml (fig 4) and in western blot on human samples at 1 ug/ml (fig 2). Biochimie (2012) ncbi
rabbit polyclonal
  • western blot; human; fig 3
In order to study the contribution of miR-26a to breast carcinogenesis, Invitrogen MTDH antibody (Invitrogen, 40-6400) was used in western blot on human samples (fig 3). Carcinogenesis (2011) ncbi
Abnova
mouse polyclonal
  • immunohistochemistry - paraffin section; mouse; fig 6
Abnova MTDH antibody (Abnova, H00092140-B01P) was used in immunohistochemistry - paraffin section on mouse samples (fig 6). Cancer Res (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; fig 7
  • western blot; human; fig 9
Abnova MTDH antibody (Abnova, H00092140-D01) was used in immunohistochemistry on human samples (fig 7) and in western blot on human samples (fig 9). Oncotarget (2015) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, Bethyl MTDH antibody (Bethyl, A303-144A) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 1
Proteintech Group MTDH antibody (Proteintech, 13860-1-AP) was used in western blot on human samples (fig 1). Oncotarget (2016) ncbi
Abcam
rabbit monoclonal (EP4445)
  • immunohistochemistry - paraffin section; human; fig 1
  • western blot; human; fig 3
Abcam MTDH antibody (Abcam, ab124789) was used in immunohistochemistry - paraffin section on human samples (fig 1) and in western blot on human samples (fig 3). Int J Gynecol Cancer (2015) ncbi
Cell Signaling Technology
mouse monoclonal (2F11C3)
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology MTDH antibody (Cell Signaling, 9596) was used in western blot on human samples (fig 1d). Sci Signal (2017) ncbi
mouse monoclonal (2F11C3)
  • western blot; human; fig 5
Cell Signaling Technology MTDH antibody (Cell Signaling Tech, CST-9596) was used in western blot on human samples (fig 5). Oncotarget (2016) ncbi
mouse monoclonal (2F11C3)
  • western blot; human; fig 1
  • immunohistochemistry - paraffin section; mouse; fig 6
Cell Signaling Technology MTDH antibody (Cell Signaling Technology, 9596s) was used in western blot on human samples (fig 1) and in immunohistochemistry - paraffin section on mouse samples (fig 6). Cancer Res (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human
EMD Millipore MTDH antibody (Millipore, AB2989) was used in western blot on human samples . PLoS Genet (2014) ncbi
Articles Reviewed
  1. Shimizu K, Fukushima H, Ogura K, Lien E, Nihira N, Zhang J, et al. The SCF?-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis. Sci Signal. 2017;10: pubmed publisher
  2. Liu L, Guan H, Li Y, Ying Z, Wu J, Zhu X, et al. Astrocyte Elevated Gene 1 Interacts with Acetyltransferase p300 and c-Jun To Promote Tumor Aggressiveness. Mol Cell Biol. 2017;37: pubmed publisher
  3. Yao J, Qin L, Miao S, Wang X, Wu X. Overexpression of miR-506 suppresses proliferation and promotes apoptosis of osteosarcoma cells by targeting astrocyte elevated gene-1. Oncol Lett. 2016;12:1840-1848 pubmed
  4. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  5. Zou M, Zhu W, Wang L, Shi L, Gao R, Ou Y, et al. AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition. Oncotarget. 2016;7:13122-38 pubmed publisher
  6. Zhou C, Wang C, Yu A, Wang Q, Zhan M, Tang J, et al. MiR-630 suppresses breast cancer progression by targeting metadherin. Oncotarget. 2016;7:1288-99 pubmed publisher
  7. Liang Y, Hu J, Li J, Liu Y, Yu J, Zhuang X, et al. Epigenetic Activation of TWIST1 by MTDH Promotes Cancer Stem-like Cell Traits in Breast Cancer. Cancer Res. 2015;75:3672-80 pubmed publisher
  8. Wang Z, Cao C, Huang L, Ke Z, Luo C, Lin Z, et al. EFEMP1 promotes the migration and invasion of osteosarcoma via MMP-2 with induction by AEG-1 via NF-κB signaling pathway. Oncotarget. 2015;6:14191-208 pubmed
  9. Meng X, Yang S, Zhang Y, Wang X, Goodfellow R, Jia Y, et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem. 2015;290:11853-64 pubmed publisher
  10. Song E, Yu W, Xiong X, Kuang X, Ai Y, Xiong X. Astrocyte elevated gene-1 promotes progression of cervical squamous cell carcinoma by inducing epithelial-mesenchymal transition via Wnt signaling. Int J Gynecol Cancer. 2015;25:345-55 pubmed publisher
  11. Li P, Feng L, Chen N, Ge X, Lv X, Lu K, et al. Metadherin contributes to the pathogenesis of chronic lymphocytic leukemia partially through Wnt/β-catenin pathway. Med Oncol. 2015;32:479 pubmed publisher
  12. Suh S, Yoo J, Cui R, Kaur B, Huebner K, Lee T, et al. FHIT suppresses epithelial-mesenchymal transition (EMT) and metastasis in lung cancer through modulation of microRNAs. PLoS Genet. 2014;10:e1004652 pubmed publisher
  13. Luparello C, Longo A, Vetrano M. Exposure to cadmium chloride influences astrocyte-elevated gene-1 (AEG-1) expression in MDA-MB231 human breast cancer cells. Biochimie. 2012;94:207-13 pubmed publisher
  14. Zhang B, Liu X, He J, Zhou C, Guo M, He M, et al. Pathologically decreased miR-26a antagonizes apoptosis and facilitates carcinogenesis by targeting MTDH and EZH2 in breast cancer. Carcinogenesis. 2011;32:2-9 pubmed publisher