This is a Validated Antibody Database (VAD) review about human MSTN, based on 17 published articles (read how Labome selects the articles), using MSTN antibody in all methods. It is aimed to help Labome visitors find the most suited MSTN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MSTN synonym: GDF8; MSLHP; growth/differentiation factor 8

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (A-1)
  • western blot knockout validation; sheep; fig 4
Santa Cruz Biotechnology MSTN antibody (Santa Cruz, sc-398333) was used in western blot knockout validation on sheep samples (fig 4). PLoS ONE (2015) ncbi
R&D Systems
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s7
In order to study the benefits of heterochronic parabiosis in young and old mice, R&D Systems MSTN antibody (R&D Systems, AF788) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig s7). Nat Commun (2016) ncbi
goat polyclonal
  • immunoprecipitation; human
R&D Systems MSTN antibody (R&D systems, BAF788) was used in immunoprecipitation on human samples . Skelet Muscle (2015) ncbi
goat polyclonal
  • western blot; mouse; fig 3
R&D Systems MSTN antibody (R&D Systems, AF788) was used in western blot on mouse samples (fig 3). J Biol Chem (2015) ncbi
goat polyclonal
  • western blot; Rhesus monkey; fig s1
R&D Systems MSTN antibody (R&D Systems, AF788) was used in western blot on Rhesus monkey samples (fig s1). FASEB J (2015) ncbi
Abcam
rabbit polyclonal
  • western blot; human; 1:100; fig s3
Abcam MSTN antibody (Abcam, ab98337) was used in western blot on human samples at 1:100 (fig s3). Scand J Med Sci Sports (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 7
Abcam MSTN antibody (abcam, Ab71808) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 7). Oxid Med Cell Longev (2015) ncbi
Invitrogen
mouse monoclonal (6H12)
  • western blot; mouse; 1:1000; fig 4
In order to study how overexpression of latent TGFbeta binding protein 4 in muscle ameliorates muscular dystrophy through TGFbeta and myostatin, Invitrogen MSTN antibody (Pierce, MA5-15486) was used in western blot on mouse samples at 1:1000 (fig 4). PLoS Genet (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-1)
  • western blot knockout validation; sheep; fig 4
Santa Cruz Biotechnology MSTN antibody (Santa Cruz, sc-398333) was used in western blot knockout validation on sheep samples (fig 4). PLoS ONE (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
In order to study how overexpression of latent TGFbeta binding protein 4 in muscle ameliorates muscular dystrophy through TGFbeta and myostatin, EMD Millipore MSTN antibody (EMD Millipore, AB3239-I) was used in western blot on mouse samples at 1:1000 (fig 4). PLoS Genet (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:100
EMD Millipore MSTN antibody (Millipore, AB3239) was used in immunohistochemistry - frozen section on human samples at 1:100. Age (Dordr) (2014) ncbi
rabbit polyclonal
  • western blot; mouse
EMD Millipore MSTN antibody (Millipore, AB3239) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:100
EMD Millipore MSTN antibody (Millipore, AB3239) was used in immunohistochemistry - frozen section on human samples at 1:100. J Nutr (2014) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore MSTN antibody (Millipore, AB3239) was used in western blot on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; mouse
EMD Millipore MSTN antibody (Millipore, AB3239) was used in western blot on mouse samples . Mol Pharm (2013) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore MSTN antibody (Millipore, AB3239) was used in western blot on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; sheep; 1:1,000
EMD Millipore MSTN antibody (Millipore, AB3239) was used in western blot on sheep samples at 1:1,000. Mol Biol Rep (2013) ncbi
rabbit polyclonal
  • western blot; scFv; 1:1000
EMD Millipore MSTN antibody (Millipore, ab3239) was used in western blot on scFv samples at 1:1000. Genomics Proteomics Bioinformatics (2012) ncbi
rabbit polyclonal
  • western blot; rat
EMD Millipore MSTN antibody (EMD Millipore, AB3239) was used in western blot on rat samples . Nutr Metab (Lond) (2012) ncbi
Articles Reviewed
  1. Rebo J, Mehdipour M, Gathwala R, Causey K, Liu Y, Conboy M, et al. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood. Nat Commun. 2016;7:13363 pubmed publisher
  2. Lamar K, Bogdanovich S, Gardner B, Gao Q, Miller T, Earley J, et al. Overexpression of Latent TGF? Binding Protein 4 in Muscle Ameliorates Muscular Dystrophy through Myostatin and TGF?. PLoS Genet. 2016;12:e1006019 pubmed publisher
  3. Crispo M, Mulet A, Tesson L, Barrera N, Cuadro F, Dos Santos Neto P, et al. Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. PLoS ONE. 2015;10:e0136690 pubmed publisher
  4. Nilsen T, Thorsen L, Fosså S, Wiig M, Kirkegaard C, Skovlund E, et al. Effects of strength training on muscle cellular outcomes in prostate cancer patients on androgen deprivation therapy. Scand J Med Sci Sports. 2016;26:1026-35 pubmed publisher
  5. Bergen H, Farr J, Vanderboom P, Atkinson E, White T, Singh R, et al. Myostatin as a mediator of sarcopenia versus homeostatic regulator of muscle mass: insights using a new mass spectrometry-based assay. Skelet Muscle. 2015;5:21 pubmed publisher
  6. Seto D, Kandarian S, Jackman R. A Key Role for Leukemia Inhibitory Factor in C26 Cancer Cachexia. J Biol Chem. 2015;290:19976-86 pubmed publisher
  7. Kim J, Ku S, Kim K, Kim S, Han M, Kim G, et al. Schisandrae Fructus Supplementation Ameliorates Sciatic Neurectomy-Induced Muscle Atrophy in Mice. Oxid Med Cell Longev. 2015;2015:872428 pubmed publisher
  8. O Connell K, Guo W, Serra C, Beck M, Wachtman L, Hoggatt A, et al. The effects of an ActRIIb receptor Fc fusion protein ligand trap in juvenile simian immunodeficiency virus-infected rhesus macaques. FASEB J. 2015;29:1165-75 pubmed publisher
  9. Snijders T, Verdijk L, Smeets J, McKay B, Senden J, Hartgens F, et al. The skeletal muscle satellite cell response to a single bout of resistance-type exercise is delayed with aging in men. Age (Dordr). 2014;36:9699 pubmed publisher
  10. Hauerslev S, Vissing J, Krag T. Muscle atrophy reversed by growth factor activation of satellite cells in a mouse muscle atrophy model. PLoS ONE. 2014;9:e100594 pubmed publisher
  11. Snijders T, Verdijk L, McKay B, Smeets J, van Kranenburg J, Groen B, et al. Acute dietary protein intake restriction is associated with changes in myostatin expression after a single bout of resistance exercise in healthy young men. J Nutr. 2014;144:137-45 pubmed publisher
  12. Breitbart A, Scharf G, Duncker D, Widera C, Gottlieb J, Vogel A, et al. Highly specific detection of myostatin prodomain by an immunoradiometric sandwich assay in serum of healthy individuals and patients. PLoS ONE. 2013;8:e80454 pubmed publisher
  13. Wang G, Lu Q. A nitrate ester of sedative alkyl alcohol improves muscle function and structure in a murine model of Duchenne muscular dystrophy. Mol Pharm. 2013;10:3862-70 pubmed publisher
  14. Hauerslev S, Sveen M, Vissing J, Krag T. Protein turnover and cellular stress in mildly and severely affected muscles from patients with limb girdle muscular dystrophy type 2I. PLoS ONE. 2013;8:e66929 pubmed publisher
  15. Lu J, Wei C, Zhang X, Xu L, Zhang S, Liu J, et al. The effect of myostatin silencing by lentiviral-mediated RNA interference on goat fetal fibroblasts. Mol Biol Rep. 2013;40:4101-8 pubmed publisher
  16. Kornfeld S, Biggar K, Storey K. Differential expression of mature microRNAs involved in muscle maintenance of hibernating little brown bats, Myotis lucifugus: a model of muscle atrophy resistance. Genomics Proteomics Bioinformatics. 2012;10:295-301 pubmed publisher
  17. Sharma N, Castorena C, Cartee G. Greater insulin sensitivity in calorie restricted rats occurs with unaltered circulating levels of several important myokines and cytokines. Nutr Metab (Lond). 2012;9:90 pubmed publisher