This is a Validated Antibody Database (VAD) review about human MSI2, based on 9 published articles (read how Labome selects the articles), using MSI2 antibody in all methods. It is aimed to help Labome visitors find the most suited MSI2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MSI2 synonym: MSI2H; RNA-binding protein Musashi homolog 2; musashi homolog 2; musashi-2

Knockout validation
Abcam
rabbit monoclonal (EP1305Y)
  • immunohistochemistry - paraffin section; human; loading ...; fig s1a
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig s5c
In order to demonstrate that Musashi is crucial for pancreatic cancer progression, Abcam MSI2 antibody (Abcam, ab76148) was used in immunohistochemistry - paraffin section on human samples (fig s1a) and in immunohistochemistry knockout validation on mouse samples at 1:500 (fig s5c). Nature (2016) ncbi
Abcam
rabbit monoclonal (EP1305Y)
  • other; human; loading ...; fig 4c
Abcam MSI2 antibody (Abcam, ab76148) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
rabbit monoclonal (EP1305Y)
  • immunoprecipitation; human; loading ...; fig 6a
  • western blot; human; loading ...; fig 6a
Abcam MSI2 antibody (Abcam, Ab76148) was used in immunoprecipitation on human samples (fig 6a) and in western blot on human samples (fig 6a). Nat Genet (2017) ncbi
rabbit monoclonal (EP1305Y)
  • western blot; human; loading ...; fig 3b
In order to test if MSI2 modulates FLT3 expression, Abcam MSI2 antibody (Abcam, EP1305Y) was used in western blot on human samples (fig 3b). Leuk Res (2017) ncbi
rabbit monoclonal (EP1305Y)
  • western blot; mouse; loading ...; fig 1f
Abcam MSI2 antibody (Abcam, ab76148) was used in western blot on mouse samples (fig 1f). Cell Death Dis (2016) ncbi
rabbit monoclonal (EP1305Y)
  • immunohistochemistry - paraffin section; human; loading ...; fig s1a
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig s5c
In order to demonstrate that Musashi is crucial for pancreatic cancer progression, Abcam MSI2 antibody (Abcam, ab76148) was used in immunohistochemistry - paraffin section on human samples (fig s1a) and in immunohistochemistry knockout validation on mouse samples at 1:500 (fig s5c). Nature (2016) ncbi
rabbit monoclonal (EP1305Y)
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, Abcam MSI2 antibody (Abcam, ab76148) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse
Abcam MSI2 antibody (Abcam, ab50829) was used in immunohistochemistry - paraffin section on mouse samples and in western blot on mouse samples . Biol Reprod (2014) ncbi
Invitrogen
mouse monoclonal (2C11)
  • western blot; human; loading ...; fig 2g
In order to show that that MSI2 directly regulates estrogen receptor 1 in breast cancer cells, Invitrogen MSI2 antibody (ThermoFisher Scientific, MA5-15821) was used in western blot on human samples (fig 2g). Oncogene (2017) ncbi
Bethyl
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 3a
In order to show that that MSI2 directly regulates estrogen receptor 1 in breast cancer cells, Bethyl MSI2 antibody (Bethyl Laboratories, A303-215A) was used in immunoprecipitation on human samples (fig 3a). Oncogene (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 3a
In order to show that that MSI2 directly regulates estrogen receptor 1 in breast cancer cells, Bethyl MSI2 antibody (Bethyl Laboratories, A303-216A) was used in immunoprecipitation on human samples (fig 3a). Oncogene (2017) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 2c
Proteintech Group MSI2 antibody (Proteintech, 107701AP) was used in western blot on human samples (fig 2c). Science (2017) ncbi
GeneTex
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.5 ug/ml
In order to develop reagents to study RNA binding proteins, GeneTex MSI2 antibody (GeneTex, GTX117808) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.5 ug/ml. Mol Cell (2016) ncbi
Articles Reviewed
  1. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  2. Chavali P, Stojic L, Meredith L, Joseph N, Nahorski M, Sanford T, et al. Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication. Science. 2017;357:83-88 pubmed publisher
  3. Vu L, Prieto C, Amin E, Chhangawala S, Krivtsov A, Calvo Vidal M, et al. Functional screen of MSI2 interactors identifies an essential role for SYNCRIP in myeloid leukemia stem cells. Nat Genet. 2017;49:866-875 pubmed publisher
  4. Hattori A, McSkimming D, Kannan N, Ito T. RNA binding protein MSI2 positively regulates FLT3 expression in myeloid leukemia. Leuk Res. 2017;54:47-54 pubmed publisher
  5. Kang M, Jeong K, Kim W, Lee H, Gong G, Suh N, et al. Musashi RNA-binding protein 2 regulates estrogen receptor 1 function in breast cancer. Oncogene. 2017;36:1745-1752 pubmed publisher
  6. Fujiwara T, Zhou J, Ye S, Zhao H. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis. 2016;7:e2300 pubmed publisher
  7. Fox R, Lytle N, Jaquish D, Park F, Ito T, Bajaj J, et al. Image-based detection and targeting of therapy resistance in pancreatic adenocarcinoma. Nature. 2016;534:407-411 pubmed publisher
  8. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  9. Sutherland J, Fraser B, Sobinoff A, Pye V, Davidson T, Siddall N, et al. Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression. Biol Reprod. 2014;90:92 pubmed publisher