This is a Validated Antibody Database (VAD) review about human MLST8, based on 11 published articles (read how Labome selects the articles), using MLST8 antibody in all methods. It is aimed to help Labome visitors find the most suited MLST8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MLST8 synonym: GBL; GbetaL; LST8; POP3; WAT1; target of rapamycin complex subunit LST8; TORC subunit LST8; gable; mammalian lethal with SEC13 protein 8; protein GbetaL

Knockout validation
Cell Signaling Technology
rabbit monoclonal (86B8)
  • western blot knockout validation; mouse; 1:1000; loading ...
Cell Signaling Technology MLST8 antibody (Cell signaling, 3274) was used in western blot knockout validation on mouse samples at 1:1000. Nature (2017) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; 1:1000; fig s3
In order to elucidate the mechanisms by which mutant IDH1/2 contributes to oncogenesis, Invitrogen MLST8 antibody (Thermo Scientific, PA5-17442) was used in western blot on human samples at 1:1000 (fig s3). Nat Commun (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1s1b
Cell Signaling Technology MLST8 antibody (Cell signaling, 3227) was used in western blot on human samples at 1:1000 (fig 1s1b). elife (2019) ncbi
rabbit monoclonal (86B8)
  • immunoprecipitation; human; loading ...; fig s4a
Cell Signaling Technology MLST8 antibody (Cell Signaling Technology, 3274) was used in immunoprecipitation on human samples (fig s4a). Cell Metab (2019) ncbi
rabbit monoclonal (86B8)
  • western blot; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology MLST8 antibody (CST, 3274S) was used in western blot on human samples at 1:1000 (fig 3b). Sci Adv (2018) ncbi
rabbit monoclonal (86B8)
  • western blot knockout validation; mouse; 1:1000; loading ...
Cell Signaling Technology MLST8 antibody (Cell signaling, 3274) was used in western blot knockout validation on mouse samples at 1:1000. Nature (2017) ncbi
rabbit monoclonal (86B8)
  • western blot; mouse; 1:1000; loading ...; fig 9e
Cell Signaling Technology MLST8 antibody (Cell Signaling, 3274) was used in western blot on mouse samples at 1:1000 (fig 9e). J Neurosci (2017) ncbi
rabbit monoclonal (86B8)
  • western blot; human; loading ...; fig 3a
In order to research the roles of HSF1 and mTORC1 in proteotoxic stress and cell growth, Cell Signaling Technology MLST8 antibody (Cell Signaling, 3274) was used in western blot on human samples (fig 3a). Nat Cell Biol (2016) ncbi
rabbit monoclonal (86B8)
  • western blot; mouse; 1:1000; fig 1
Cell Signaling Technology MLST8 antibody (Cell Signaling, 3274) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
rabbit monoclonal (86B8)
  • western blot; human; fig 5
Cell Signaling Technology MLST8 antibody (Cell Signaling, 3274) was used in western blot on human samples (fig 5). PLoS ONE (2015) ncbi
rabbit monoclonal (86B8)
  • western blot; rat; fig 10
Cell Signaling Technology MLST8 antibody (Cell Signaling, 3274) was used in western blot on rat samples (fig 10). PLoS ONE (2015) ncbi
rabbit monoclonal (86B8)
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology MLST8 antibody (Cell Signaling, 3274) was used in western blot on human samples (fig 1a). J Biol Chem (2015) ncbi
Articles Reviewed
  1. Jewell J, Fu V, Hong A, Yu F, Meng D, Melick C, et al. GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. elife. 2019;8: pubmed publisher
  2. Son S, Park S, Lee H, Siddiqi F, Lee J, Menzies F, et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab. 2019;29:192-201.e7 pubmed publisher
  3. NGUYEN J, Ray C, Fox A, Mendonça D, Kim J, Krebsbach P. Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration. Sci Adv. 2018;4:eaao5838 pubmed publisher
  4. Wang B, Jie Z, Joo D, Ordureau A, Liu P, Gan W, et al. TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling. Nature. 2017;545:365-369 pubmed publisher
  5. Hussain R, Macklin W. Integrin-Linked Kinase (ILK) Deletion Disrupts Oligodendrocyte Development by Altering Cell Cycle. J Neurosci. 2017;37:397-412 pubmed publisher
  6. Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
  7. Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
  8. Kim K, Qiang L, Hayden M, Sparling D, Purcell N, Pajvani U. mTORC1-independent Raptor prevents hepatic steatosis by stabilizing PHLPP2. Nat Commun. 2016;7:10255 pubmed publisher
  9. Yasuda K, Takahashi M, Mori N. Mdm20 Modulates Actin Remodeling through the mTORC2 Pathway via Its Effect on Rictor Expression. PLoS ONE. 2015;10:e0142943 pubmed publisher
  10. Nishida H, Ikegami A, Kaneko C, Kakuma H, Nishi H, Tanaka N, et al. Dexamethasone and BCAA Failed to Modulate Muscle Mass and mTOR Signaling in GH-Deficient Rats. PLoS ONE. 2015;10:e0128805 pubmed publisher
  11. Fonseca B, Zakaria C, Jia J, Graber T, Svitkin Y, Tahmasebi S, et al. La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1). J Biol Chem. 2015;290:15996-6020 pubmed publisher