This is a Validated Antibody Database (VAD) review about human MLH1, based on 53 published articles (read how Labome selects the articles), using MLH1 antibody in all methods. It is aimed to help Labome visitors find the most suited MLH1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MLH1 synonym: COCA2; FCC2; HNPCC; HNPCC2; hMLH1

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (B-12)
  • western blot knockout validation; human; loading ...; fig 4a
Santa Cruz Biotechnology MLH1 antibody (Santa Cruz, sc-271978) was used in western blot knockout validation on human samples (fig 4a). Cell Death Dis (2020) ncbi
BD Biosciences
mouse monoclonal (G168-15)
  • western blot knockout validation; human; 1:250; loading ...; fig 5b
BD Biosciences MLH1 antibody (BD, 551091) was used in western blot knockout validation on human samples at 1:250 (fig 5b). elife (2019) ncbi
Abcam
mouse monoclonal (G168-15)
  • immunocytochemistry; human; loading ...; fig 2b
Abcam MLH1 antibody (Abcam, ab14206) was used in immunocytochemistry on human samples (fig 2b). J Biol Chem (2019) ncbi
domestic rabbit monoclonal (EPR3894)
  • immunohistochemistry - frozen section; mouse; 1:1500; loading ...; fig 1a
Abcam MLH1 antibody (Abcam, ab92312) was used in immunohistochemistry - frozen section on mouse samples at 1:1500 (fig 1a). Carcinogenesis (2018) ncbi
domestic rabbit monoclonal (EPR3894)
  • western blot; mouse; fig s4c
Abcam MLH1 antibody (Abcam, ab92312) was used in western blot on mouse samples (fig s4c). Cell (2018) ncbi
domestic rabbit monoclonal (EPR3894)
  • western blot; mouse; fig s4c
Abcam MLH1 antibody (Abcam, ab92312) was used in western blot on mouse samples (fig s4c). Cell (2018) ncbi
domestic rabbit monoclonal (EPR3894)
  • immunohistochemistry - paraffin section; human; loading ...; fig s5e-h
  • western blot; human; loading ...; fig s3a-b
In order to investigate the oncogenic functions of CHD4, Abcam MLH1 antibody (Abcam, ab92312) was used in immunohistochemistry - paraffin section on human samples (fig s5e-h) and in western blot on human samples (fig s3a-b). Cancer Cell (2017) ncbi
domestic rabbit monoclonal (EPR3894)
  • immunohistochemistry - paraffin section; rat; 1:1600; fig st11
In order to outline the protocols for antibodies used for immunohistochemical studies, Abcam MLH1 antibody (Abcam, ab92312) was used in immunohistochemistry - paraffin section on rat samples at 1:1600 (fig st11). J Toxicol Pathol (2017) ncbi
mouse monoclonal (G168-15)
  • immunocytochemistry; mouse; 1:20; fig 1
Abcam MLH1 antibody (Abcam, ab14206) was used in immunocytochemistry on mouse samples at 1:20 (fig 1). PLoS Genet (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-12)
  • western blot knockout validation; human; loading ...; fig 4a
Santa Cruz Biotechnology MLH1 antibody (Santa Cruz, sc-271978) was used in western blot knockout validation on human samples (fig 4a). Cell Death Dis (2020) ncbi
OriGene
mouse monoclonal (OTI1C1)
  • immunohistochemistry - paraffin section; human; 1:150; loading ...; fig 1
OriGene MLH1 antibody (zhongshan jiqiao, OTI1C1) was used in immunohistochemistry - paraffin section on human samples at 1:150 (fig 1). Cancer Med (2017) ncbi
mouse monoclonal (OTI1C1)
  • immunohistochemistry - paraffin section; human; 1:150; loading ...; fig 1
OriGene MLH1 antibody (zhongshan jiqiao, OTI1C1) was used in immunohistochemistry - paraffin section on human samples at 1:150 (fig 1). PLoS ONE (2017) ncbi
MilliporeSigma
mouse monoclonal (M1)
  • western blot; mouse; loading ...; fig 3b
  • immunocytochemistry; human; loading ...; fig 4a
MilliporeSigma MLH1 antibody (Sigma-Aldrich, WH0004292M2) was used in western blot on mouse samples (fig 3b) and in immunocytochemistry on human samples (fig 4a). Cancer Discov (2017) ncbi
Dako
mouse monoclonal (ES05)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1
Dako MLH1 antibody (Dako, IR079) was used in immunohistochemistry - paraffin section on human samples (fig 1). Cancer Manag Res (2020) ncbi
mouse monoclonal (ES05)
  • immunohistochemistry - paraffin section; human; fig 2
Dako MLH1 antibody (Dako, IR079) was used in immunohistochemistry - paraffin section on human samples (fig 2). BMC Cancer (2019) ncbi
mouse monoclonal (ES05)
  • immunohistochemistry - paraffin section; human; loading ...; tbl 2
In order to determine the prognostic impact of parameters in peritoneal carcinomatosis from colorectal cancer, Dako MLH1 antibody (Dako, ES05) was used in immunohistochemistry - paraffin section on human samples (tbl 2). Am J Surg (2017) ncbi
mouse monoclonal (ES05)
  • immunocytochemistry; human; 1:50; loading ...; fig 2c
In order to determine the effect of chronic smoking on repair gene expression in the normal oral mucosa of chronic smokers and never smokers, Dako MLH1 antibody (DakoCorporation, ES05) was used in immunocytochemistry on human samples at 1:50 (fig 2c). Arch Oral Biol (2017) ncbi
mouse monoclonal (ES05)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; tbl 1
Dako MLH1 antibody (Dako, M3640) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 1). Br J Cancer (2016) ncbi
mouse monoclonal (ES05)
  • immunohistochemistry - paraffin section; human; 1:50; fig s1
In order to develop an "antigen relaxing" method to improve routine detection of scarce antigens in formalin-fixed, paraffin-embedded material, Dako MLH1 antibody (Dako, ES05) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig s1). Appl Immunohistochem Mol Morphol (2016) ncbi
Cell Signaling Technology
mouse monoclonal (4C9C7)
  • western blot; human; 1:1000; loading ...; fig e10b
Cell Signaling Technology MLH1 antibody (Cell Signaling, 3515) was used in western blot on human samples at 1:1000 (fig e10b). Nature (2019) ncbi
mouse monoclonal (4C9C7)
  • western blot; human; 1:1000; loading ...
Cell Signaling Technology MLH1 antibody (Cell Signaling, 3515) was used in western blot on human samples at 1:1000. elife (2019) ncbi
mouse monoclonal (4C9C7)
  • western blot; human; loading ...; fig s1e
Cell Signaling Technology MLH1 antibody (Cell Signaling, 4C9C7) was used in western blot on human samples (fig s1e). Science (2017) ncbi
domestic rabbit monoclonal (D38G9)
  • western blot; human; fig 5
Cell Signaling Technology MLH1 antibody (Signaling Technology, 4256) was used in western blot on human samples (fig 5). J Cell Mol Med (2016) ncbi
mouse monoclonal (4C9C7)
  • western blot; human; 1:1000; fig 1g
In order to elucidate the function of TRIM29 in double stranded break repair, Cell Signaling Technology MLH1 antibody (Cell signalling, 3,515) was used in western blot on human samples at 1:1000 (fig 1g). Nat Commun (2015) ncbi
mouse monoclonal (4C9C7)
  • western blot; human; fig s4
In order to report the effects of new temozolomide analogues on glioblastoma multiforme, Cell Signaling Technology MLH1 antibody (Cell Signaling, 3515) was used in western blot on human samples (fig s4). Mol Cancer Ther (2015) ncbi
mouse monoclonal (4C9C7)
  • western blot; human
Cell Signaling Technology MLH1 antibody (Cell Signaling Technology, 3515) was used in western blot on human samples . elife (2014) ncbi
BD Biosciences
mouse monoclonal (G168-15)
  • immunocytochemistry; mouse; loading ...; fig 3f
BD Biosciences MLH1 antibody (BD Pharmingen, 550838) was used in immunocytochemistry on mouse samples (fig 3f). iScience (2020) ncbi
mouse monoclonal (G168-15)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4f
BD Biosciences MLH1 antibody (BD, 551092) was used in immunocytochemistry on mouse samples at 1:100 (fig 4f). Cell Death Dis (2020) ncbi
mouse monoclonal (G168-728)
  • western blot; human; loading ...; fig 1s1c
BD Biosciences MLH1 antibody (BD, 554073) was used in western blot on human samples (fig 1s1c). elife (2019) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry; mouse; 1:50; loading ...; fig s6d
BD Biosciences MLH1 antibody (BD Biosciences, 550838) was used in immunohistochemistry on mouse samples at 1:50 (fig s6d). Nucleic Acids Res (2019) ncbi
mouse monoclonal (G168-15)
  • western blot knockout validation; human; 1:250; loading ...; fig 5b
BD Biosciences MLH1 antibody (BD, 551091) was used in western blot knockout validation on human samples at 1:250 (fig 5b). elife (2019) ncbi
mouse monoclonal (G168-15)
  • immunocytochemistry; mouse; 1:200; loading ...; fig 4i
BD Biosciences MLH1 antibody (BD, 551092) was used in immunocytochemistry on mouse samples at 1:200 (fig 4i). Sci Adv (2019) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; 1:10; loading ...; fig 1
BD Biosciences MLH1 antibody (BD, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:10 (fig 1). Oncol Lett (2016) ncbi
mouse monoclonal (G168-15)
  • immunocytochemistry; African green monkey; 1:200; loading ...; fig 4
BD Biosciences MLH1 antibody (BD Biosciences, 550838) was used in immunocytochemistry on African green monkey samples at 1:200 (fig 4). PLoS Pathog (2017) ncbi
mouse monoclonal (G168-728)
  • western blot; human; loading ...; fig s1
In order to characterize autophagy induction by thiopurines, BD Biosciences MLH1 antibody (BD Pharmingen, 554073) was used in western blot on human samples (fig s1). Oncotarget (2016) ncbi
mouse monoclonal (G168-15)
  • immunocytochemistry; mouse; 1:50
BD Biosciences MLH1 antibody (Pharmingen, 551092) was used in immunocytochemistry on mouse samples at 1:50. EMBO Rep (2016) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry; mouse; 1:100; fig 3
BD Biosciences MLH1 antibody (Becton Dickinson, 551091) was used in immunohistochemistry on mouse samples at 1:100 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (G168-728)
  • immunohistochemistry; human; 1:400; fig s4b
In order to discuss mechanisms involved in the resistance of androgen receptor-targeted therapies, BD Biosciences MLH1 antibody (Abcam, 554073) was used in immunohistochemistry on human samples at 1:400 (fig s4b). Nat Med (2016) ncbi
mouse monoclonal (G168-728)
  • immunohistochemistry; human; 1:100; loading ...
BD Biosciences MLH1 antibody (BD Pharmingen, G168-728) was used in immunohistochemistry on human samples at 1:100. JAMA Oncol (2016) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; tbl 1
In order to evaluate the use of e-cadherin as a biomarker for medullary carcinoma, BD Biosciences MLH1 antibody (BD Bioscience, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:50 (tbl 1). Hum Pathol (2015) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 4
BD Biosciences MLH1 antibody (BD Biosciences, 550838) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 4). EMBO Mol Med (2015) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry; human; 1:50
In order to compare the immunohistochemical and genetic profiles found in the three types of endometrial carcinomas, BD Biosciences MLH1 antibody (BD, G168-15) was used in immunohistochemistry on human samples at 1:50. Gynecol Oncol (2015) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry; human; 1:25; loading ...
In order to assess the detection of the BRAF mutation by immunohistochemistry on tissue microarray, BD Biosciences MLH1 antibody (BD Biosciences, G168-15) was used in immunohistochemistry on human samples at 1:25. Appl Immunohistochem Mol Morphol (2016) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 3
BD Biosciences MLH1 antibody (BD Pharmingen, 551092) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 3). Cell Death Dis (2015) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human
BD Biosciences MLH1 antibody (BD Pharmingen, 550838) was used in immunohistochemistry - paraffin section on human samples . Cancer Genet (2014) ncbi
mouse monoclonal (G168-15)
  • western blot; human
In order to explore DNA repair responses in human pluripotent stem cells, BD Biosciences MLH1 antibody (BD Biosciences, 550838) was used in western blot on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; 1:100
BD Biosciences MLH1 antibody (PharMingen, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:100. PLoS ONE (2014) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; 1:30
In order to discuss the diagnosis of colorectal cancer and the need for genetic testing for Lynch Syndrome in young patients, BD Biosciences MLH1 antibody (Biosciences Company, 551092) was used in immunohistochemistry - paraffin section on human samples at 1:30. BMC Surg (2014) ncbi
mouse monoclonal (G168-728)
  • immunoprecipitation; human
  • western blot; human
BD Biosciences MLH1 antibody (BD Biosciences, G168-728) was used in immunoprecipitation on human samples and in western blot on human samples . Mol Cancer (2014) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry; human
BD Biosciences MLH1 antibody (BD Bio sciences, G-168-15) was used in immunohistochemistry on human samples . Br J Cancer (2014) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; fig 2
In order to report the first case of filiform polyposis associated with adenomas developed on filiform polyps and invasive colonic adenocarcinoma in a patient without history of inflammatory bowel disease, BD Biosciences MLH1 antibody (BD, G168-15) was used in immunohistochemistry - paraffin section on human samples (fig 2). Pathol Res Pract (2014) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; 1:30; tbl 1
BD Biosciences MLH1 antibody (BD Biosciences, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:30 (tbl 1). PLoS ONE (2013) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; human; 1:25
BD Biosciences MLH1 antibody (BD Pharmingen, G168-15) was used in immunohistochemistry - paraffin section on human samples at 1:25. BMC Gastroenterol (2013) ncbi
mouse monoclonal (G168-15)
  • immunohistochemistry - paraffin section; mouse
BD Biosciences MLH1 antibody (BD Biosciences, G168-15) was used in immunohistochemistry - paraffin section on mouse samples . PLoS Genet (2013) ncbi
mouse monoclonal (G168-15)
  • immunocytochemistry; mouse; 1:50
BD Biosciences MLH1 antibody (BD Pharmingen, G168-1) was used in immunocytochemistry on mouse samples at 1:50. Development (2007) ncbi
Articles Reviewed
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  2. Shao W, Wang C, Wang L, Xiao F, Xiao D, Yang H, et al. A Hereditable Mutation of MSH2 Gene Associated with Lynch Syndrome in a Five Generation Chinese Family. Cancer Manag Res. 2020;12:1469-1482 pubmed publisher
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  25. Alves M, Carta C, de Barros P, Issa J, Nunes F, Almeida J. Repair genes expression profile of MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers. Arch Oral Biol. 2017;73:60-65 pubmed publisher
  26. Chaabane W, Appell M. Interconnections between apoptotic and autophagic pathways during thiopurine-induced toxicity in cancer cells: the role of reactive oxygen species. Oncotarget. 2016;7:75616-75634 pubmed publisher
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  32. Beltran H, Prandi D, Mosquera J, Benelli M, Puca L, Cyrta J, et al. Divergent clonal evolution of castration-resistant neuroendocrine prostate cancer. Nat Med. 2016;22:298-305 pubmed publisher
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  35. Yang F, Silber S, Leu N, Oates R, Marszalek J, Skaletsky H, et al. TEX11 is mutated in infertile men with azoospermia and regulates genome-wide recombination rates in mouse. EMBO Mol Med. 2015;7:1198-210 pubmed publisher
  36. Masuda Y, Takahashi H, Sato S, Tomomori Sato C, Saraf A, Washburn M, et al. TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin. Nat Commun. 2015;6:7299 pubmed publisher
  37. Scalia C, Gendusa R, Cattoretti G. A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection. Appl Immunohistochem Mol Morphol. 2016;24:436-46 pubmed publisher
  38. Geels Y, van der Putten L, van Tilborg A, Lurkin I, Zwarthoff E, Pijnenborg J, et al. Immunohistochemical and genetic profiles of endometrioid endometrial carcinoma arising from atrophic endometrium. Gynecol Oncol. 2015;137:245-51 pubmed publisher
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  40. Gao X, Ma W, Nie J, Zhang C, Zhang J, Yao G, et al. A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation. Cell Death Dis. 2015;6:e1610 pubmed publisher
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  42. Ramirez Y, Mladek A, Phillips R, Gynther M, Rautio J, Ross A, et al. Evaluation of novel imidazotetrazine analogues designed to overcome temozolomide resistance and glioblastoma regrowth. Mol Cancer Ther. 2015;14:111-9 pubmed publisher
  43. Lin B, Gupta D, Heinen C. Human pluripotent stem cells have a novel mismatch repair-dependent damage response. J Biol Chem. 2014;289:24314-24 pubmed publisher
  44. Koppensteiner R, Samartzis E, Noske A, von Teichman A, Dedes I, Gwerder M, et al. Effect of MRE11 loss on PARP-inhibitor sensitivity in endometrial cancer in vitro. PLoS ONE. 2014;9:e100041 pubmed publisher
  45. Chen J, Miller B, Furano A. Repair of naturally occurring mismatches can induce mutations in flanking DNA. elife. 2014;3:e02001 pubmed publisher
  46. Baiocchi G, Portolani N, Vermi W, Baronchelli C, Gheza F, Zogno C, et al. Lynch syndrome from a surgeon perspective: retrospective study of clinical impact of mismatch repair protein expression analysis in colorectal cancer patients less than 50 years old. BMC Surg. 2014;14:9 pubmed publisher
  47. Hinrichsen I, Ernst B, Nuber F, Passmann S, Schäfer D, Steinke V, et al. Reduced migration of MLH1 deficient colon cancer cells depends on SPTAN1. Mol Cancer. 2014;13:11 pubmed publisher
  48. Malesci A, Basso G, Bianchi P, Fini L, Grizzi F, Celesti G, et al. Molecular heterogeneity and prognostic implications of synchronous advanced colorectal neoplasia. Br J Cancer. 2014;110:1228-35 pubmed publisher
  49. Boulagnon C, Jazeron J, Diaz Cives A, Ehrhard F, Bouche O, Diebold M. Filiform polyposis: A benign entity? Case report and literature review. Pathol Res Pract. 2014;210:189-93 pubmed publisher
  50. Egoavil C, Alenda C, Castillejo A, Paya A, Peiro G, Sanchez Heras A, et al. Prevalence of Lynch syndrome among patients with newly diagnosed endometrial cancers. PLoS ONE. 2013;8:e79737 pubmed publisher
  51. Donada M, Bonin S, Barbazza R, Pettirosso D, Stanta G. Management of stage II colon cancer - the use of molecular biomarkers for adjuvant therapy decision. BMC Gastroenterol. 2013;13:36 pubmed publisher
  52. Link J, Jahn D, Schmitt J, Göb E, Baar J, Ortega S, et al. The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse. PLoS Genet. 2013;9:e1003261 pubmed publisher
  53. Takada Y, Isono K, Shinga J, Turner J, Kitamura H, Ohara O, et al. Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. Development. 2007;134:579-90 pubmed