This is a Validated Antibody Database (VAD) review about human MIF, based on 9 published articles (read how Labome selects the articles), using MIF antibody in all methods. It is aimed to help Labome visitors find the most suited MIF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MIF synonym: GIF; GLIF; MMIF

Abcam
domestic rabbit monoclonal (EPR12463)
  • immunocytochemistry; human; 1:250; loading ...; fig 11a
  • western blot; human; 1:1000; loading ...; fig 1a, 3a
Abcam MIF antibody (Abcam, ab175189) was used in immunocytochemistry on human samples at 1:250 (fig 11a) and in western blot on human samples at 1:1000 (fig 1a, 3a). J Biol Chem (2018) ncbi
domestic rabbit monoclonal (EPR12463)
  • chromatin immunoprecipitation; human; fig 6
  • immunocytochemistry; human; fig 5
  • western blot; human; fig 3
Abcam MIF antibody (Abcam, ab175189) was used in chromatin immunoprecipitation on human samples (fig 6), in immunocytochemistry on human samples (fig 5) and in western blot on human samples (fig 3). Stem Cell Reports (2016) ncbi
domestic rabbit monoclonal (EPR12463)
  • western blot; human; fig 5f
Abcam MIF antibody (Abcam, ab175189) was used in western blot on human samples (fig 5f). Oncotarget (2016) ncbi
mouse monoclonal
  • western blot; human; 1:2000; fig 5
Abcam MIF antibody (abcam, ab55445) was used in western blot on human samples at 1:2000 (fig 5). Mol Med Rep (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-2)
  • western blot; mouse; loading ...; fig 6c,d
Santa Cruz Biotechnology MIF antibody (Santa Cruz, sc-271631) was used in western blot on mouse samples (fig 6c,d). Sci Rep (2015) ncbi
mouse monoclonal (D-2)
  • immunohistochemistry - paraffin section; human; 1:200
Santa Cruz Biotechnology MIF antibody (Santa Cruz Biotechnology, D-2) was used in immunohistochemistry - paraffin section on human samples at 1:200. Ann Diagn Pathol (2014) ncbi
R&D Systems
mouse monoclonal (12302)
  • ELISA; human; loading ...; tbl 5
R&D Systems MIF antibody (R&D Systems, MAB289) was used in ELISA on human samples (tbl 5). PLoS ONE (2016) ncbi
Abnova
mouse monoclonal (2A10-4D3)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, Abnova MIF antibody (Abnova, H00004282-M01) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:1200; loading ...; fig 5b
  • western blot; mouse; 1:3000; loading ...; fig 5a
In order to test if macrophage migration inhibitory factor regulates the stress-induced erythropoietic response, Invitrogen MIF antibody (Thermo Fisher Scientific, PA5-27343) was used in immunohistochemistry - paraffin section on mouse samples at 1:1200 (fig 5b) and in western blot on mouse samples at 1:3000 (fig 5a). Histochem Cell Biol (2016) ncbi
Articles Reviewed
  1. De R, Sarkar S, Mazumder S, Debsharma S, Siddiqui A, Saha S, et al. Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-κB signaling. J Biol Chem. 2018;293:19740-19760 pubmed publisher
  2. Kim J, Choi J, Hahn H, Lee Y, Yu D, Kim J. Association of Macrophage Migration Inhibitory Factor Polymorphisms with Total Plasma IgE Levels in Patients with Atopic Dermatitis in Korea. PLoS ONE. 2016;11:e0162477 pubmed publisher
  3. Yao Y, Deng Q, Song W, Zhang H, Li Y, Yang Y, et al. MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia. Stem Cell Reports. 2016;7:249-62 pubmed publisher
  4. Vignjević Petrinović S, Budec M, Marković D, Gotic M, Mitrović Ajtić O, Mojsilovic S, et al. Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. Histochem Cell Biol. 2016;146:311-24 pubmed publisher
  5. Minna E, Romeo P, Dugo M, De Cecco L, Todoerti K, Pilotti S, et al. miR-451a is underexpressed and targets AKT/mTOR pathway in papillary thyroid carcinoma. Oncotarget. 2016;7:12731-47 pubmed publisher
  6. Zeng J, Quan J, Xia X. Transient transfection of macrophage migration inhibitory factor small interfering RNA disrupts the biological behavior of oral squamous carcinoma cells. Mol Med Rep. 2016;13:174-80 pubmed publisher
  7. Nishida K, Watanabe H, Ogaki S, Kodama A, Tanaka R, Imafuku T, et al. Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-associated acute kidney injury. Sci Rep. 2015;5:14471 pubmed publisher
  8. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  9. Villarreal Calderon R, Luévano González A, Aragón Flores M, Zhu H, Yuan Y, Xiang Q, et al. Antral atrophy, intestinal metaplasia, and preneoplastic markers in Mexican children with Helicobacter pylori-positive and Helicobacter pylori-negative gastritis. Ann Diagn Pathol. 2014;18:129-35 pubmed publisher