This is a Validated Antibody Database (VAD) review about human MICA, based on 16 published articles (read how Labome selects the articles), using MICA antibody in all methods. It is aimed to help Labome visitors find the most suited MICA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MICA synonym: MIC-A; PERB11.1

BioLegend
mouse monoclonal (6D4)
  • flow cytometry; human; loading ...; fig 1a
BioLegend MICA antibody (BioLegend, 320906) was used in flow cytometry on human samples (fig 1a). J Exp Clin Cancer Res (2021) ncbi
mouse monoclonal (6D4)
  • flow cytometry; human; loading ...; fig 3
BioLegend MICA antibody (BioLegend, 320906) was used in flow cytometry on human samples (fig 3). Stem Cell Reports (2020) ncbi
mouse monoclonal (6D4)
  • flow cytometry; human; loading ...; fig 2b
BioLegend MICA antibody (Biolegend, 320906) was used in flow cytometry on human samples (fig 2b). Biosci Rep (2019) ncbi
mouse monoclonal (6D4)
  • immunocytochemistry; human; loading ...; fig 1b
  • immunohistochemistry; human; loading ...; fig 2a
In order to study the expression of MHC class I chain-related protein A and B in tumour and normal tissue, BioLegend MICA antibody (BioLegend, 6D4) was used in immunocytochemistry on human samples (fig 1b) and in immunohistochemistry on human samples (fig 2a). Br J Cancer (2017) ncbi
mouse monoclonal (6D4)
  • flow cytometry; human; loading ...; fig s3g
BioLegend MICA antibody (BioLegend, 6D4) was used in flow cytometry on human samples (fig s3g). Oncogene (2017) ncbi
mouse monoclonal (6D4)
  • flow cytometry; human; fig 4
BioLegend MICA antibody (Biolegend, 6D4) was used in flow cytometry on human samples (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (6D4)
  • flow cytometry; human; fig 7
In order to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses, BioLegend MICA antibody (Biolegend, 6D4) was used in flow cytometry on human samples (fig 7). Mucosal Immunol (2016) ncbi
mouse monoclonal (6D4)
  • flow cytometry; human
BioLegend MICA antibody (Biolegend, 6D4) was used in flow cytometry on human samples . Immunology (2014) ncbi
mouse monoclonal (6D4)
BioLegend MICA antibody (Biolegend, 6D4) was used . Int Immunol (2014) ncbi
R&D Systems
mouse monoclonal (159227)
  • flow cytometry; human; loading ...; fig 2c
R&D Systems MICA antibody (Biotechne, 159,227) was used in flow cytometry on human samples (fig 2c). Oncoimmunology (2022) ncbi
mouse monoclonal (159227)
  • flow cytometry; human; loading ...; fig 2b
R&D Systems MICA antibody (R&D Systems, 159227) was used in flow cytometry on human samples (fig 2b). BMC Biol (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2C10)
  • western blot; human; 1:250; loading ...; fig s4c
Santa Cruz Biotechnology MICA antibody (Santa Cruz, sc-23870) was used in western blot on human samples at 1:250 (fig s4c). Nat Commun (2020) ncbi
mouse monoclonal (2C10)
  • flow cytometry; human; loading ...; fig 1
Santa Cruz Biotechnology MICA antibody (Santa, 2C10) was used in flow cytometry on human samples (fig 1). J Biol Chem (2018) ncbi
mouse monoclonal (2C10)
  • flow cytometry; human; loading ...; fig 3a
  • western blot; human; 1:100; loading ...; fig 5
Santa Cruz Biotechnology MICA antibody (Santa Cruz, 2C10) was used in flow cytometry on human samples (fig 3a) and in western blot on human samples at 1:100 (fig 5). Front Immunol (2016) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry - paraffin section; human; 1:800; fig 1
Santa Cruz Biotechnology MICA antibody (Santa Cruz Biotechnology, sc-137242) was used in immunohistochemistry - paraffin section on human samples at 1:800 (fig 1). Cancer Sci (2016) ncbi
Invitrogen
mouse monoclonal (6D4)
  • immunocytochemistry; human
  • immunohistochemistry; human
Invitrogen MICA antibody (eBioscience, 6D4) was used in immunocytochemistry on human samples and in immunohistochemistry on human samples . Exp Mol Pathol (2014) ncbi
Articles Reviewed
  1. Secchiari F, Nu xf1 ez S, Sierra J, Ziblat A, Regge M, Raffo Iraolagoitia X, et al. The MICA-NKG2D axis in clear cell renal cell carcinoma bolsters MICA as target in immuno-oncology. Oncoimmunology. 2022;11:2104991 pubmed publisher
  2. Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
  3. Hirano M, Imai Y, Kaito Y, Murayama T, Sato K, Ishida T, et al. Small-molecule HDAC and Akt inhibitors suppress tumor growth and enhance immunotherapy in multiple myeloma. J Exp Clin Cancer Res. 2021;40:110 pubmed publisher
  4. Mahameed M, Boukeileh S, Obiedat A, Darawshi O, Dipta P, Rimon A, et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun. 2020;11:1304 pubmed publisher
  5. Suzuki D, Flahou C, Yoshikawa N, Stirblyte I, Hayashi Y, Sawaguchi A, et al. iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity. Stem Cell Reports. 2020;14:49-59 pubmed publisher
  6. Tang S, Fu H, Xu Q, Zhou Y. miR-20a regulates sensitivity of colorectal cancer cells to NK cells by targeting MICA. Biosci Rep. 2019;: pubmed publisher
  7. McCarthy M, Moncayo G, Hiron T, Jakobsen N, Valli A, Soga T, et al. Purine nucleotide metabolism regulates expression of the human immune ligand MICA. J Biol Chem. 2018;293:3913-3924 pubmed publisher
  8. Ghadially H, Brown L, Lloyd C, Lewis L, LEWIS A, Dillon J, et al. MHC class I chain-related protein A and B (MICA and MICB) are predominantly expressed intracellularly in tumour and normal tissue. Br J Cancer. 2017;116:1208-1217 pubmed publisher
  9. Moncayo G, Lin D, McCarthy M, Watson A, O Callaghan C. MICA Expression Is Regulated by Cell Adhesion and Contact in a FAK/Src-Dependent Manner. Front Immunol. 2016;7:687 pubmed publisher
  10. Sauer M, Schuldner M, Hoffmann N, Cetintas A, Reiners K, Shatnyeva O, et al. CBP/p300 acetyltransferases regulate the expression of NKG2D ligands on tumor cells. Oncogene. 2017;36:933-941 pubmed publisher
  11. Ritter C, Fan K, Paulson K, Nghiem P, Schrama D, Becker J. Reversal of epigenetic silencing of MHC class I chain-related protein A and B improves immune recognition of Merkel cell carcinoma. Sci Rep. 2016;6:21678 pubmed publisher
  12. Tsukagoshi M, Wada S, Yokobori T, Altan B, Ishii N, Watanabe A, et al. Overexpression of natural killer group 2 member D ligands predicts favorable prognosis in cholangiocarcinoma. Cancer Sci. 2016;107:116-22 pubmed publisher
  13. Yawata N, Selva K, Liu Y, Tan K, Lee A, Siak J, et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection. Mucosal Immunol. 2016;9:159-70 pubmed publisher
  14. Ikeshita S, Miyatake Y, Otsuka N, Kasahara M. MICA/B expression in macrophage foam cells infiltrating atherosclerotic plaques. Exp Mol Pathol. 2014;97:171-5 pubmed publisher
  15. Vogel K, Thomann S, Vogel B, Schuster P, Schmidt B. Both plasmacytoid dendritic cells and monocytes stimulate natural killer cells early during human herpes simplex virus type 1 infections. Immunology. 2014;143:588-600 pubmed publisher
  16. Watanabe M, Kudo Y, Kawano M, Nakayama M, Nakamura K, Kameda M, et al. NKG2D functions as an activating receptor on natural killer cells in the common marmoset (Callithrix jacchus). Int Immunol. 2014;26:597-606 pubmed publisher