This is a Validated Antibody Database (VAD) review about human MGEA5, based on 7 published articles (read how Labome selects the articles), using MGEA5 antibody in all methods. It is aimed to help Labome visitors find the most suited MGEA5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MGEA5 synonym: MEA5; NCOAT; OGA; protein O-GlcNAcase; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; beta-hexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase

Knockout validation
Bethyl
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 2
  • western blot; human; loading ...; fig 1b
  • western blot knockout validation; mouse; loading ...; fig 1b
  • immunoprecipitation; mouse; loading ...; fig 2
Bethyl MGEA5 antibody (Bethyl Laboratories, A304-345A) was used in immunoprecipitation on human samples (fig 2), in western blot on human samples (fig 1b), in western blot knockout validation on mouse samples (fig 1b), and in immunoprecipitation on mouse samples (fig 2). Glycobiology (2017) ncbi
Abcam
rabbit monoclonal (EPR7154(B))
  • western blot knockout validation; mouse; loading ...; fig 1b
  • immunoprecipitation; mouse; loading ...; fig 2
  • immunoprecipitation; human; loading ...; fig 2
  • western blot; human; loading ...; fig 1b
Abcam MGEA5 antibody (Abcam, ab124807) was used in western blot knockout validation on mouse samples (fig 1b), in immunoprecipitation on mouse samples (fig 2), in immunoprecipitation on human samples (fig 2), and in western blot on human samples (fig 1b). Glycobiology (2017) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; rat; 1:500; fig 1
Proteintech Group MGEA5 antibody (Proteintech, 14711-1-AP) was used in western blot on rat samples at 1:500 (fig 1)PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3c
In order to investigate the role of O-GlcNAc in insulin-glucose homeostasis, Proteintech Group MGEA5 antibody (ProteinTech Group, 14711-1-AP) was used in western blot on mouse samples (fig 3c)J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; rat
Proteintech Group MGEA5 antibody (Proteintech, 14711-1-AP) was used in western blot on rat samples Physiol Behav (2015) ncbi
Bethyl
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 2
  • western blot; human; loading ...; fig 1b
  • western blot knockout validation; mouse; loading ...; fig 1b
  • immunoprecipitation; mouse; loading ...; fig 2
Bethyl MGEA5 antibody (Bethyl Laboratories, A304-345A) was used in immunoprecipitation on human samples (fig 2), in western blot on human samples (fig 1b), in western blot knockout validation on mouse samples (fig 1b), and in immunoprecipitation on mouse samples (fig 2)Glycobiology (2017) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; fig 7
  • western blot; rat; fig 2
In order to determine the role of protein O-GlcNAcylation in skeletal muscle, Sigma-Aldrich MGEA5 antibody (Sigma, SAB4200267) was used in western blot on human samples (fig 7) and in western blot on rat samples (fig 2)Physiol Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig s3
Sigma-Aldrich MGEA5 antibody (Sigma-Aldrich, SAB4200311) was used in western blot on human samples (fig s3)Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; rat
In order to test if exhausting in vivo treadmill running induces dephosphorylation, and if there are reciprocal changes in O-GlcNAcylation, of MLC2, Sigma-Aldrich MGEA5 antibody (Sigma-Aldrich, SAB4200267) was used in western blot on rat samples Physiol Rep (2015) ncbi
Abcam
rabbit monoclonal (EPR7154(B))
  • western blot knockout validation; mouse; loading ...; fig 1b
  • immunoprecipitation; mouse; loading ...; fig 2
  • immunoprecipitation; human; loading ...; fig 2
  • western blot; human; loading ...; fig 1b
Abcam MGEA5 antibody (Abcam, ab124807) was used in western blot knockout validation on mouse samples (fig 1b), in immunoprecipitation on mouse samples (fig 2), in immunoprecipitation on human samples (fig 2), and in western blot on human samples (fig 1b)Glycobiology (2017) ncbi
Articles Reviewed
  1. Jennifer A Groves et al. (2017). "Characterization of tools to detect and enrich human and mouse O-GlcNAcase.".PMID 28595377
  2. Kristin Halvorsen Hortemo et al. (2016). "Exercise training increases protein O-GlcNAcylation in rat skeletal muscle".PMID 27664189
  3. Chiaki Nagai-Okatani et al. (2016). "Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure".PMID 27281159
  4. Cédric Chaveroux et al. (2016). "Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway".PMID 27255611
  5. Kristin Halvorsen Hortemo et al. (2015). "Exhausting treadmill running causes dephosphorylation of sMLC2 and reduced level of myofilament MLCK2 in slow twitch rat soleus muscle".PMID 25713325
  6. Chithra Keembiyehetty et al. (2015). "Conditional knock-out reveals a requirement for O-linked N-Acetylglucosaminase (O-GlcNAcase) in metabolic homeostasis".PMID 25596529
  7. Ruth B S Harris et al. (2015). "Hexosamine biosynthetic pathway activity in leptin resistant sucrose-drinking rats".PMID 25446204