This is a Validated Antibody Database (VAD) review about human MFGE8, based on 6 published articles (read how Labome selects the articles), using MFGE8 antibody in all methods. It is aimed to help Labome visitors find the most suited MFGE8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MFGE8 synonym: BA46; EDIL1; HMFG; HsT19888; MFG-E8; MFGM; OAcGD3S; SED1; SPAG10; hP47; lactadherin; O-acetyl disialoganglioside synthase; breast epithelial antigen BA46; medin; sperm associated antigen 10; sperm surface protein hP47

Knockout validation
MBL International
(18A2-G10)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 7a
  • western blot; mouse; 1:2000; loading ...; fig 3b
In order to observe that cardiac myofibroblasts efficiently engulf dead cells, MBL International MFGE8 antibody (MBL, 18A2-G10) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 7a) and in western blot on mouse samples at 1:2000 (fig 3b). J Clin Invest (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-5)
  • western blot; human; loading ...; fig 1a
Santa Cruz Biotechnology MFGE8 antibody (Santa Cruz, sc271574) was used in western blot on human samples (fig 1a). PLoS ONE (2016) ncbi
mouse monoclonal (MFG-06)
  • western blot; mouse; fig 12
In order to determine the induction of liver injury in mice due to molecular circuits of diclofenac revealed by immunogenomics, Santa Cruz Biotechnology MFGE8 antibody (santa Cruz, sc-8029) was used in western blot on mouse samples (fig 12). Oncotarget (2016) ncbi
Abcam
mouse monoclonal (SPM291)
  • immunocytochemistry; human; 1:1000
  • western blot; human
Abcam MFGE8 antibody (Abcam, ab17787) was used in immunocytochemistry on human samples at 1:1000 and in western blot on human samples . Fertil Steril (2014) ncbi
MBL International
(18A2-G10)
  • western blot; mouse; fig 2a
In order to describe extracellular vesicle subpopulations secreted by fat cells, MBL International MFGE8 antibody (MBL, D199-3) was used in western blot on mouse samples (fig 2a). J Extracell Vesicles (2017) ncbi
(18A2-G10)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 7a
  • western blot; mouse; 1:2000; loading ...; fig 3b
In order to observe that cardiac myofibroblasts efficiently engulf dead cells, MBL International MFGE8 antibody (MBL, 18A2-G10) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 7a) and in western blot on mouse samples at 1:2000 (fig 3b). J Clin Invest (2017) ncbi
(18A2-G10)
  • immunohistochemistry; mouse; 1:250; fig 2
In order to study the role of microglial phagocytosis of living photoreceptors in retinal degeneration, MBL International MFGE8 antibody (MBL, D199-3) was used in immunohistochemistry on mouse samples at 1:250 (fig 2). EMBO Mol Med (2015) ncbi
Articles Reviewed
  1. Durcin M, Fleury A, Taillebois E, Hilairet G, Krupova Z, Henry C, et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles. 2017;6:1305677 pubmed publisher
  2. Nakaya M, Watari K, Tajima M, Nakaya T, Matsuda S, Ohara H, et al. Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. J Clin Invest. 2017;127:383-401 pubmed publisher
  3. Dismuke W, Klingeborn M, Stamer W. Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone. PLoS ONE. 2016;11:e0165326 pubmed publisher
  4. Lee E, Oh J, Selvaraj S, Park S, Choi M, Spanel R, et al. Immunogenomics reveal molecular circuits of diclofenac induced liver injury in mice. Oncotarget. 2016;7:14983-5017 pubmed publisher
  5. Zhao L, Zabel M, Wang X, Ma W, Shah P, Fariss R, et al. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med. 2015;7:1179-97 pubmed publisher
  6. Schmitz C, Yu L, Bocca S, Anderson S, Cunha Filho J, Rhavi B, et al. Role for the endometrial epithelial protein MFG-E8 and its receptor integrin ?v?3 in human implantation: results of an in vitro trophoblast attachment study using established human cell lines. Fertil Steril. 2014;101:874-82 pubmed publisher