This is a Validated Antibody Database (VAD) review about human MFF, based on 10 published articles (read how Labome selects the articles), using MFF antibody in all methods. It is aimed to help Labome visitors find the most suited MFF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MFF synonym: C2orf33; EMPF2; GL004; mitochondrial fission factor

Knockout validation
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; fig 1a
Proteintech Group MFF antibody (Proteintech, noca) was used in western blot knockout validation on human samples (fig 1a). J Cell Biol (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s4a
In order to study the function of AMP-activated protein kinase in stress-induced mitochondrial fission, Proteintech Group MFF antibody (Protein Tech, 17090-1-AP) was used in western blot knockout validation on mouse samples (fig s4a). Science (2016) ncbi
others
  • western blot; mouse; loading ...; fig s1c
 MFF antibody (Abcam, ab129075) was used in western blot on mouse samples (fig s1c). Cell Rep (2018) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6e
Proteintech Group MFF antibody (Proteintech, 17090-1-AP) was used in western blot on mouse samples at 1:1000 (fig 6e). Nat Commun (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1a
  • western blot; mouse; loading ...; fig 4a
In order to research the protective effect of Mfn2 on dopaminergic neurons, Proteintech Group MFF antibody (Proteintech, 17090-1-AP) was used in western blot on human samples (fig 1a) and in western blot on mouse samples (fig 4a). Biochim Biophys Acta Mol Basis Dis (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig sf1
In order to research the mechanisms of mitochondrial division, Proteintech Group MFF antibody (Proteintech, 17090-1-AP) was used in western blot on human samples (fig sf1). Nature (2016) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 4b
In order to evaluate an approach for correcting mitochondrial fusion, Proteintech Group MFF antibody (Proteintech, 17090-1-ap) was used in western blot on mouse samples (fig 4b). Nature (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; fig 6
In order to study affects of acute minimization of brown adipose tissue function by increased reliance on muscle based thermogenesis, Proteintech Group MFF antibody (Proteintech, 17090-1-AP) was used in western blot on mouse samples at 1:500 (fig 6). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; human; fig 1a
Proteintech Group MFF antibody (Proteintech, noca) was used in western blot knockout validation on human samples (fig 1a). J Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s4a
In order to study the function of AMP-activated protein kinase in stress-induced mitochondrial fission, Proteintech Group MFF antibody (Protein Tech, 17090-1-AP) was used in western blot knockout validation on mouse samples (fig s4a). Science (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig s2
In order to analyze delay of neurodegeneration by preventing stress-induced OPA1 processing in mitochondria by loss of OMA1, Proteintech Group MFF antibody (ProteinTech, 17090-1-AP) was used in western blot on mouse samples (fig s2). J Cell Biol (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse
  • western blot; mouse
Proteintech Group MFF antibody (Proteintech, 17090-1-AP) was used in immunoprecipitation on mouse samples and in western blot on mouse samples . Cell Signal (2015) ncbi
Abcam
rabbit monoclonal (EPR7360)
  • western blot; mouse; loading ...; fig s1c
Abcam MFF antibody (Abcam, ab129075) was used in western blot on mouse samples (fig s1c). Cell Rep (2018) ncbi
Articles Reviewed
  1. Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
  2. Velázquez Villegas L, Perino A, Lemos V, Zietak M, Nomura M, Pols T, et al. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue. Nat Commun. 2018;9:245 pubmed publisher
  3. Zhao F, Wang W, Wang C, Siedlak S, Fujioka H, Tang B, et al. Mfn2 protects dopaminergic neurons exposed to paraquat both in vitro and in vivo: Implications for idiopathic Parkinson's disease. Biochim Biophys Acta Mol Basis Dis. 2017;1863:1359-1370 pubmed publisher
  4. Lee J, Westrate L, Wu H, Page C, Voeltz G. Multiple dynamin family members collaborate to drive mitochondrial division. Nature. 2016;540:139-143 pubmed publisher
  5. Franco A, Kitsis R, Fleischer J, Gavathiotis E, Kornfeld O, Gong G, et al. Correcting mitochondrial fusion by manipulating mitofusin conformations. Nature. 2016;540:74-79 pubmed publisher
  6. Bal N, Maurya S, Singh S, Wehrens X, Periasamy M. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem. 2016;291:17247-57 pubmed publisher
  7. Otera H, Miyata N, Kuge O, Mihara K. Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling. J Cell Biol. 2016;212:531-44 pubmed publisher
  8. Toyama E, Herzig S, Courchet J, Lewis T, Losón O, Hellberg K, et al. Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016;351:275-281 pubmed publisher
  9. Korwitz A, Merkwirth C, Richter Dennerlein R, Tröder S, Sprenger H, Quirós P, et al. Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria. J Cell Biol. 2016;212:157-66 pubmed publisher
  10. Ducommun S, Deak M, Sumpton D, Ford R, Núñez Galindo A, Kussmann M, et al. Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate. Cell Signal. 2015;27:978-88 pubmed publisher