This is a Validated Antibody Database (VAD) review about human METTL3, based on 15 published articles (read how Labome selects the articles), using METTL3 antibody in all methods. It is aimed to help Labome visitors find the most suited METTL3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
METTL3 synonym: IME4; M6A; MT-A70; Spo8; hMETTL3

Knockout validation
Abcam
domestic rabbit monoclonal (EPR18810)
  • western blot knockout validation; mouse; loading ...; fig s1a
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot knockout validation on mouse samples (fig s1a). Science (2020) ncbi
Abcam
domestic rabbit monoclonal (EPR18810)
  • western blot; mouse; loading ...; fig 1e
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on mouse samples (fig 1e). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • RNA immunoprecipitation; human; loading ...; fig 6g
Abcam METTL3 antibody (Abcam, ab195352) was used in RNA immunoprecipitation on human samples (fig 6g). Cancer Cell Int (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • immunohistochemistry; human; loading ...; fig 2d
  • western blot; human; loading ...; fig 3a
Abcam METTL3 antibody (Abcam, ab195352) was used in immunohistochemistry on human samples (fig 2d) and in western blot on human samples (fig 3a). Oncol Lett (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; 1:1000; loading ...; fig 1e
Abcam METTL3 antibody (Abcam, ab19552) was used in western blot on human samples at 1:1000 (fig 1e). Cancer Cell Int (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • RNA immunoprecipitation; human; loading ...; fig 3i
  • western blot; human; loading ...; fig s4f
Abcam METTL3 antibody (Abcam, ab195352) was used in RNA immunoprecipitation on human samples (fig 3i) and in western blot on human samples (fig s4f). Mol Cancer (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; 1:1000; fig 3h
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on human samples at 1:1000 (fig 3h). FEBS Open Bio (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; fig s3d
Abcam METTL3 antibody (Abcam, Ab195352) was used in western blot on human samples (fig s3d). Front Cell Dev Biol (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; loading ...; fig 6d
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on human samples (fig 6d). Redox Biol (2021) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; 1:1200; loading ...; fig 3c
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on human samples at 1:1200 (fig 3c). Aging (Albany NY) (2020) ncbi
domestic rabbit monoclonal (EPR18810)
  • immunohistochemistry; mouse; 1:100-1:500; loading ...; fig 2b
Abcam METTL3 antibody (Abcam, ab195352) was used in immunohistochemistry on mouse samples at 1:100-1:500 (fig 2b). elife (2020) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot knockout validation; mouse; loading ...; fig s1a
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot knockout validation on mouse samples (fig s1a). Science (2020) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; 1:1000; loading ...
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on human samples at 1:1000. Nat Commun (2019) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; mouse; loading ...; fig 3a
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on mouse samples (fig 3a). Nucleic Acids Res (2019) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; loading ...; fig 2i, 7f
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on human samples (fig 2i, 7f). Nat Commun (2019) ncbi
domestic rabbit monoclonal (EPR18810)
  • western blot; human; fig 3a
Abcam METTL3 antibody (Abcam, ab195352) was used in western blot on human samples (fig 3a). Biochem Biophys Res Commun (2017) ncbi
Articles Reviewed
  1. Lü Z, Liu H, Song N, Liang Y, Zhu J, Chen J, et al. METTL14 aggravates podocyte injury and glomerulopathy progression through N6-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12:881 pubmed publisher
  2. Yue M, Liu T, Yan G, Luo X, Wang L. LINC01605, regulated by the EP300-SMYD2 complex, potentiates the binding between METTL3 and SPTBN2 in colorectal cancer. Cancer Cell Int. 2021;21:504 pubmed publisher
  3. Wen J, Zhang G, Meng Y, Zhang L, Jiang M, Yu Z. RNA m6A methyltransferase METTL3 promotes colorectal cancer cell proliferation and invasion by regulating Snail expression. Oncol Lett. 2021;22:711 pubmed publisher
  4. Zhao C, Ling X, Xia Y, Yan B, Guan Q. The m6A methyltransferase METTL3 controls epithelial-mesenchymal transition, migration and invasion of breast cancer through the MALAT1/miR-26b/HMGA2 axis. Cancer Cell Int. 2021;21:441 pubmed publisher
  5. Liu Z, Wang T, She Y, Wu K, Gu S, Li L, et al. N6-methyladenosine-modified circIGF2BP3 inhibits CD8+ T-cell responses to facilitate tumor immune evasion by promoting the deubiquitination of PD-L1 in non-small cell lung cancer. Mol Cancer. 2021;20:105 pubmed publisher
  6. Pan Z, Wang B, Hou D, You R, Wang X, Xie W, et al. METTL3 mediates bone marrow mesenchymal stem cell adipogenesis to promote chemoresistance in acute myeloid leukaemia. FEBS Open Bio. 2021;11:1659-1672 pubmed publisher
  7. Hua Y, Zhang K, Sheng J, Ning Z, Li Y, Shi W, et al. NUCB1 Suppresses Growth and Shows Additive Effects With Gemcitabine in Pancreatic Ductal Adenocarcinoma via the Unfolded Protein Response. Front Cell Dev Biol. 2021;9:641836 pubmed publisher
  8. Ma L, Chen T, Zhang X, Miao Y, Tian X, Yu K, et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol. 2021;38:101801 pubmed publisher
  9. Yang Z, Jiang X, Li D, Jiang X. HBXIP promotes gastric cancer via METTL3-mediated MYC mRNA m6A modification. Aging (Albany NY). 2020;12:24967-24982 pubmed publisher
  10. Xi L, Carroll T, Matos I, Luo J, Polak L, Pasolli H, et al. m6A RNA methylation impacts fate choices during skin morphogenesis. elife. 2020;9: pubmed publisher
  11. Liu J, Dou X, Chen C, Chen C, Liu C, Xu M, et al. N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription. Science. 2020;367:580-586 pubmed publisher
  12. Chen R, Chen X, Xia L, Zhang J, Pan Z, Ma X, et al. N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis. Nat Commun. 2019;10:4695 pubmed publisher
  13. Song T, Yang Y, Wei H, Xie X, Lu J, Zeng Q, et al. Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation. Nucleic Acids Res. 2019;: pubmed publisher
  14. Zhang J, Bai R, Li M, Ye H, Wu C, Wang C, et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun. 2019;10:1858 pubmed publisher
  15. Du M, Zhang Y, Mao Y, Mou J, Zhao J, Xue Q, et al. MiR-33a suppresses proliferation of NSCLC cells via targeting METTL3 mRNA. Biochem Biophys Res Commun. 2017;482:582-589 pubmed publisher