This is a Validated Antibody Database (VAD) review about human MED12, based on 7 published articles (read how Labome selects the articles), using MED12 antibody in all methods. It is aimed to help Labome visitors find the most suited MED12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MED12 synonym: ARC240; CAGH45; FGS1; HOPA; MED12S; OHDOX; OKS; OPA1; TNRC11; TRAP230; mediator of RNA polymerase II transcription subunit 12; CAG repeat protein 45; OPA-containing protein; activator-recruited cofactor 240 kDa component; human opposite paired; mediator of RNA polymerase II transcription, subunit 12 homolog; thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); trinucleotide repeat-containing gene 11 protein

Knockout validation
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig s10
Cell Signaling Technology MED12 antibody (Cell Signaling, 4529S) was used in western blot knockout validation on human samples at 1:1000 (fig s10). Science (2014) ncbi
Bethyl
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 5a
  • western blot; human; fig 1c
Bethyl MED12 antibody (Bethyl Lab, A300-774A) was used in immunoprecipitation on human samples (fig 5a) and in western blot on human samples (fig 1c). Hum Mol Genet (2017) ncbi
rabbit polyclonal
  • western blot; human
Bethyl MED12 antibody (Bethyl Lab, A300-774A) was used in western blot on human samples . EMBO J (2015) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse
Bethyl MED12 antibody (Bethyl, A300-774A) was used in ChIP-Seq on mouse samples . Cell (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; fig 2c
  • western blot; mouse; 1:500; fig 2c
In order to elucidate the regulation of Notch by Cyclin C, Bethyl MED12 antibody (Bethyl, A300-774A) was used in immunoprecipitation on mouse samples (fig 2c) and in western blot on mouse samples at 1:500 (fig 2c). Nat Cell Biol (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
Bethyl MED12 antibody (Bethyl, A300-774A) was used in chromatin immunoprecipitation on human samples . Cell (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:50; fig 5
  • immunohistochemistry; human; 1:50; fig 1
  • western blot; human; 1:50; fig 5
Bethyl MED12 antibody (Bethyl Laboratories, A300-774A) was used in immunocytochemistry on human samples at 1:50 (fig 5), in immunohistochemistry on human samples at 1:50 (fig 1) and in western blot on human samples at 1:50 (fig 5). Endocr Relat Cancer (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig s10
Cell Signaling Technology MED12 antibody (Cell Signaling, 4529S) was used in western blot knockout validation on human samples at 1:1000 (fig s10). Science (2014) ncbi
Articles Reviewed
  1. Donnio L, Bidon B, Hashimoto S, May M, Epanchintsev A, Ryan C, et al. MED12-related XLID disorders are dose-dependent of immediate early genes (IEGs) expression. Hum Mol Genet. 2017;26:2062-2075 pubmed publisher
  2. Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, et al. The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. EMBO J. 2015;34:2885-902 pubmed publisher
  3. Qian J, Wang Q, Dose M, Pruett N, Kieffer Kwon K, Resch W, et al. B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity. Cell. 2014;159:1524-37 pubmed publisher
  4. Li N, Fassl A, Chick J, Inuzuka H, Li X, Mansour M, et al. Cyclin C is a haploinsufficient tumour suppressor. Nat Cell Biol. 2014;16:1080-91 pubmed publisher
  5. Trimarchi T, Bilal E, Ntziachristos P, Fabbri G, Dalla Favera R, Tsirigos A, et al. Genome-wide mapping and characterization of Notch-regulated long noncoding RNAs in acute leukemia. Cell. 2014;158:593-606 pubmed publisher
  6. Shaikhibrahim Z, Offermann A, Braun M, Menon R, Syring I, Nowak M, et al. MED12 overexpression is a frequent event in castration-resistant prostate cancer. Endocr Relat Cancer. 2014;21:663-75 pubmed publisher
  7. Shalem O, Sanjana N, Hartenian E, Shi X, Scott D, Mikkelson T, et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014;343:84-87 pubmed publisher