This is a Validated Antibody Database (VAD) review about human MCP-1, based on 30 published articles (read how Labome selects the articles), using MCP-1 antibody in all methods. It is aimed to help Labome visitors find the most suited MCP-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MCP-1 synonym: GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF

Knockout validation
Abcam
domestic rabbit monoclonal (EPR21025)
  • western blot knockout validation; human; loading ...; fig s9g
Abcam MCP-1 antibody (Abcam, ab214819) was used in western blot knockout validation on human samples (fig s9g). Theranostics (2021) ncbi
Invitrogen
mouse monoclonal (5D3-F7)
  • western blot; rat; 1:1000; loading ...; fig 3a
Invitrogen MCP-1 antibody (Thermo Fisher, 14-7099-81) was used in western blot on rat samples at 1:1000 (fig 3a). Brain Behav (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 6q
  • western blot; mouse; loading ...; fig 6m
Invitrogen MCP-1 antibody (ThermoFisher Scientific, PA5-34505) was used in immunohistochemistry on mouse samples (fig 6q) and in western blot on mouse samples (fig 6m). iScience (2021) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; 1:400; loading ...; fig 6c
Invitrogen MCP-1 antibody (Invitrogen, MA5-17040) was used in western blot on mouse samples at 1:400 (fig 6c). Nat Commun (2020) ncbi
mouse monoclonal (5D3-F7)
  • flow cytometry; human; loading ...; fig 3c
Invitrogen MCP-1 antibody (eBioscience, 5D3-F7) was used in flow cytometry on human samples (fig 3c). Rheumatology (Oxford) (2020) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; fig 3
In order to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis, Invitrogen MCP-1 antibody (Thermo Fisher Scientific, MA5-17040) was used in western blot on mouse samples (fig 3). J Orthop Res (2017) ncbi
domestic rabbit monoclonal (11HCLC)
  • western blot; human
In order to compare the anti-atherosclerotic potential of pepsin-pancreatin hydrolysates from unprocessed and extruded amaranth in THP-1 lipopolysaccharide-induced human macrophages, Invitrogen MCP-1 antibody (Invitrogen, 710002) was used in western blot on human samples . Proteome Sci (2014) ncbi
mouse monoclonal (5D3-F7)
  • ELISA; human; fig 1b
In order to optimize innate defense regulator derivatives with enhanced chemokine induction, Invitrogen MCP-1 antibody (eBioscience, 5D3-F7) was used in ELISA on human samples (fig 1b). J Immunol (2010) ncbi
hamsters monoclonal (2H5)
  • ELISA; human; fig 1b
  • ELISA; mouse; fig 2b
In order to optimize innate defense regulator derivatives with enhanced chemokine induction, Invitrogen MCP-1 antibody (eBioscience, 2H5) was used in ELISA on human samples (fig 1b) and in ELISA on mouse samples (fig 2b). J Immunol (2010) ncbi
Abcam
domestic rabbit monoclonal (EPR21025)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 6a
  • western blot; mouse; loading ...; fig 6b
Abcam MCP-1 antibody (Abcam, ab214819) was used in immunohistochemistry - frozen section on mouse samples (fig 6a) and in western blot on mouse samples (fig 6b). Nutrients (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig s7a
  • immunohistochemistry; human; 1:200; loading ...; fig s6c
Abcam MCP-1 antibody (Abcam, ab73680) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig s7a) and in immunohistochemistry on human samples at 1:200 (fig s6c). Theranostics (2021) ncbi
domestic rabbit monoclonal (EPR21025)
  • western blot knockout validation; human; loading ...; fig s9g
Abcam MCP-1 antibody (Abcam, ab214819) was used in western blot knockout validation on human samples (fig s9g). Theranostics (2021) ncbi
  • immunohistochemistry - paraffin section; human
Abcam MCP-1 antibody (Abcam, ab9899) was used in immunohistochemistry - paraffin section on human samples . Nature (2015) ncbi
BioLegend
mouse monoclonal (5D3-F7)
  • immunohistochemistry - paraffin section; human; loading ...; fig s7
BioLegend MCP-1 antibody (Biolegend, 502602) was used in immunohistochemistry - paraffin section on human samples (fig s7). Sci Rep (2021) ncbi
hamsters monoclonal (2H5)
  • blocking or activating experiments; mouse; ; fig 3g
  • flow cytometry; mouse; loading ...; fig 4a
BioLegend MCP-1 antibody (BioLegend, 2H5) was used in blocking or activating experiments on mouse samples at (fig 3g) and in flow cytometry on mouse samples (fig 4a). Sci Adv (2020) ncbi
mouse monoclonal (5D3-F7)
  • ELISA; human; loading ...; fig 3d
BioLegend MCP-1 antibody (BioLegend, 502604) was used in ELISA on human samples (fig 3d). Nat Commun (2018) ncbi
mouse monoclonal (5D3-F7)
In order to ask if small particle aerosols of virus would penetrate the lower respiratory tract and blanket alveoli where target cells reside, BioLegend MCP-1 antibody (BioLegend, 5D3-F7) was used . J Immunol (2017) ncbi
hamsters monoclonal (2H5)
  • immunohistochemistry - paraffin section; mouse
BioLegend MCP-1 antibody (Biolegend, 505902) was used in immunohistochemistry - paraffin section on mouse samples . Cardiovasc Res (2015) ncbi
hamsters monoclonal (2H5)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend MCP-1 antibody (Biolegend, 505902) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
Novus Biologicals
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
Novus Biologicals MCP-1 antibody (Novus, NBP2-22115) was used in immunohistochemistry - paraffin section on mouse samples (fig 5e). Cell Death Dis (2022) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig e10g
Novus Biologicals MCP-1 antibody (Novus, NBP2- 2211555) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig e10g). Nature (2019) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:4000; loading ...; fig 3f
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 2d
Novus Biologicals MCP-1 antibody (Novus Biologicals, NBP-22115) was used in immunohistochemistry - paraffin section on human samples at 1:4000 (fig 3f) and in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 2d). Nat Commun (2019) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry; mouse; loading ...; fig 3b
Novus Biologicals MCP-1 antibody (Novus Biologicals, 2D8) was used in immunohistochemistry on mouse samples (fig 3b). Diabetes (2017) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; fig 4
Novus Biologicals MCP-1 antibody (Novus, NBP2-22115S) was used in western blot on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:100
In order to investigate the relationship between macrophages and T-box transcription factor Brachyury in tumor progression and metastasis, Novus Biologicals MCP-1 antibody (Novus Biologicals, NBP2-22115) was used in immunohistochemistry - paraffin section on human samples at 1:100. Tumour Biol (2015) ncbi
R&D Systems
mouse monoclonal (23002)
  • immunohistochemistry; human; 10 ug/ml; loading ...; fig 3j
R&D Systems MCP-1 antibody (R&D systems, 23002) was used in immunohistochemistry on human samples at 10 ug/ml (fig 3j). Neuropathol Appl Neurobiol (2021) ncbi
mouse monoclonal (23002)
  • immunohistochemistry; human; 1:100; loading ...; fig s14a
R&D Systems MCP-1 antibody (R&D System, MAB2791) was used in immunohistochemistry on human samples at 1:100 (fig s14a). Adv Sci (Weinh) (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1A7B8)
  • immunohistochemistry; mouse; 1:800; loading ...; fig 5d
Santa Cruz Biotechnology MCP-1 antibody (Santa Cruz Biotechnology, sc-32819) was used in immunohistochemistry on mouse samples at 1:800 (fig 5d). Br J Pharmacol (2021) ncbi
mouse monoclonal (5J)
  • immunohistochemistry - frozen section; rhesus macaque; 1:50
Santa Cruz Biotechnology MCP-1 antibody (Santa Cruz, SC-32771) was used in immunohistochemistry - frozen section on rhesus macaque samples at 1:50. J Neuroinflammation (2013) ncbi
Bio X Cell
hamsters monoclonal (2H5)
  • blocking or activating experiments; mouse; loading ...; fig 7d
In order to report that monocytes and natural killer cells via the DNAM-1-PVR pathway control mouse cytomegalovirus infection, Bio X Cell MCP-1 antibody (BioXcell, 2H5) was used in blocking or activating experiments on mouse samples (fig 7d). J Exp Med (2016) ncbi
hamsters monoclonal (2H5)
  • blocking or activating experiments; mouse; loading ...; fig 4c
In order to propose that CCL7 is a driver of TNF-alpha-dependent T cell-mediated inflammation in lesional psoriatic skin, Bio X Cell MCP-1 antibody (BioXCel, 2H5) was used in blocking or activating experiments on mouse samples (fig 4c). Exp Dermatol (2015) ncbi
PeproTech
mouse monoclonal
  • blocking or activating experiments; human; 2.5 ug/ml; loading ...; fig 1a, 1s1d, 4s2
PeproTech MCP-1 antibody (PeproTech, 500-M71-500UG) was used in blocking or activating experiments on human samples at 2.5 ug/ml (fig 1a, 1s1d, 4s2). elife (2019) ncbi
mouse monoclonal
  • ELISA; human; fig 6c
In order to present the role of M-sec in polarized secretion of inflammatory endothelial chemokines and CCL2-mediated lymphocyte transendothelial migration, PeproTech MCP-1 antibody (PeproTech, 500-M71) was used in ELISA on human samples (fig 6c). J Leukoc Biol (2016) ncbi
Articles Reviewed
  1. Jin Y, Liu Y, Xu L, Xu J, Xiong Y, Peng Y, et al. Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis. Cell Death Dis. 2022;13:512 pubmed publisher
  2. Dai X, Liu S, Cheng L, Huang T, Guo H, Wang D, et al. Betaine Supplementation Attenuates S-Adenosylhomocysteine Hydrolase-Deficiency-Accelerated Atherosclerosis in Apolipoprotein E-Deficient Mice. Nutrients. 2022;14: pubmed publisher
  3. Ma N, Li G, Fu X. Protective role of activating transcription factor 3 against neuronal damage in rats with cerebral ischemia. Brain Behav. 2022;12:e2522 pubmed publisher
  4. Fu H, Gui Y, Liu S, Wang Y, Bastacky S, Qiao Y, et al. The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment. iScience. 2021;24:103112 pubmed publisher
  5. Zimmer T, Broekaart D, Luinenburg M, Mijnsbergen C, Anink J, Sim N, et al. Balloon cells promote immune system activation in focal cortical dysplasia type 2b. Neuropathol Appl Neurobiol. 2021;47:826-839 pubmed publisher
  6. Fang W, Sofia Acevedo D, Smart C, Zinda B, Alissa N, Warren K, et al. Expression of CCL2/CCR2 signaling proteins in breast carcinoma cells is associated with invasive progression. Sci Rep. 2021;11:8708 pubmed publisher
  7. Chu C, Lee Y, Hsieh C, Yeh C, Chao T, Chen P, et al. Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy. Theranostics. 2021;11:3624-3641 pubmed publisher
  8. Choi Y, Kim Y, Oh S, Suh K, Kim Y, Lee G, et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh). 2021;8:2002497 pubmed publisher
  9. Song M, YEKU O, Rafiq S, Purdon T, Dong X, Zhu L, et al. Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages. Nat Commun. 2020;11:6298 pubmed publisher
  10. Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
  11. Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  12. Hua X, Sun D, Zhang W, Fu J, Tong J, Sun S, et al. P7C3-A20 alleviates fatty liver by shaping gut microbiota and inducing FGF21/FGF1, via the AMP-activated protein kinase/CREB regulated transcription coactivator 2 pathway. Br J Pharmacol. 2021;178:2111-2130 pubmed publisher
  13. Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  14. Pascual García M, Bonfill Teixidor E, Planas Rigol E, Rubio Perez C, Iurlaro R, Arias A, et al. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy. Nat Commun. 2019;10:2416 pubmed publisher
  15. Ajasin D, Rao V, Wu X, Ramasamy S, Pujato M, Ruiz A, et al. CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release. elife. 2019;8: pubmed publisher
  16. Wolf D, Anto Michel N, Blankenbach H, Wiedemann A, Buscher K, Hohmann J, et al. A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense. Nat Commun. 2018;9:525 pubmed publisher
  17. Wagner J, Jaurich H, Wallner C, Abraham S, Becerikli M, Dadras M, et al. Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity. J Orthop Res. 2017;35:2425-2434 pubmed publisher
  18. Wonderlich E, Swan Z, Bissel S, Hartman A, Carney J, O Malley K, et al. Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques. J Immunol. 2017;198:1616-1626 pubmed publisher
  19. Lenac Rovis T, Kucan Brlic P, Kaynan N, Juranic Lisnic V, Brizic I, Jordan S, et al. Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection. J Exp Med. 2016;213:1835-50 pubmed publisher
  20. Portillo J, Lopez Corcino Y, Miao Y, Tang J, Sheibani N, Kern T, et al. CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes. 2017;66:483-493 pubmed publisher
  21. Barzilai S, Blecher Gonen R, Barnett Itzhaki Z, Zauberman A, Lebel Haziv Y, Amit I, et al. M-sec regulates polarized secretion of inflammatory endothelial chemokines and facilitates CCL2-mediated lymphocyte transendothelial migration. J Leukoc Biol. 2016;99:1045-55 pubmed publisher
  22. Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, et al. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A. 2016;113:E71-80 pubmed publisher
  23. Zhang L, Zhang S, Yao J, Lowery F, Zhang Q, Huang W, et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015;527:100-104 pubmed publisher
  24. Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
  25. Brunner P, Glitzner E, Reininger B, Klein I, Stary G, Mildner M, et al. CCL7 contributes to the TNF-alpha-dependent inflammation of lesional psoriatic skin. Exp Dermatol. 2015;24:522-8 pubmed publisher
  26. Chen S, Jiao J, Jiang D, Wan Z, Li L, Li K, et al. T-box transcription factor Brachyury in lung cancer cells inhibits macrophage infiltration by suppressing CCL2 and CCL4 chemokines. Tumour Biol. 2015;36:5881-90 pubmed publisher
  27. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  28. Montoya Rodríguez A, Milán Carrillo J, Dia V, Reyes Moreno C, Gonzalez de Mejia E. Pepsin-pancreatin protein hydrolysates from extruded amaranth inhibit markers of atherosclerosis in LPS-induced THP-1 macrophages-like human cells by reducing expression of proteins in LOX-1 signaling pathway. Proteome Sci. 2014;12:30 pubmed publisher
  29. Ramesh G, Santana Gould L, Inglis F, England J, Philipp M. The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia. J Neuroinflammation. 2013;10:88 pubmed publisher
  30. Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher