This is a Validated Antibody Database (VAD) review about human MCAM, based on 60 published articles (read how Labome selects the articles), using MCAM antibody in all methods. It is aimed to help Labome visitors find the most suited MCAM antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MCAM synonym: CD146; HEMCAM; METCAM; MUC18; MelCAM
Knockout validation
| Abcam domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, ab75769) was used in western blot knockout validation on mouse samples (fig 4e, 7f) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 5k). Lab Invest (2022) ncbi |
Abcam
| domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, ab75769) was used in western blot knockout validation on mouse samples (fig 4e, 7f) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 5k). Lab Invest (2022) ncbi |
| domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, ab75769) was used in immunohistochemistry on rat samples at 1:100 (fig 2). Int J Mol Sci (2021) ncbi |
| domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, ab75769) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig s3b). Sci Signal (2021) ncbi |
| domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, ab75769) was used in immunohistochemistry on human samples at 1:100. elife (2020) ncbi |
| domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, ab75769) was used in western blot on mouse samples at 1:1000 (fig 1b, 1d). Front Immunol (2020) ncbi |
| mouse monoclonal (P1H12) |
| Abcam MCAM antibody (Abcam, ab78451) was used in immunocytochemistry on human samples (fig 2a). J Clin Med (2020) ncbi |
| domestic rabbit monoclonal (EPR3208) |
| Abcam MCAM antibody (Abcam, EPR3208) was used in immunohistochemistry - paraffin section on mouse samples (fig 2d). J Neuroinflammation (2018) ncbi |
| mouse monoclonal (P1H12) |
| Abcam MCAM antibody (Abcam, ab24577) was used in immunohistochemistry on human samples (fig 1c) and in immunohistochemistry on mouse samples (fig 1c). Nat Commun (2018) ncbi |
| domestic rabbit monoclonal (EPR3208) |
| In order to describe the effects of a polyphenol-rich strawberry extract on breast cancer cells, Abcam MCAM antibody (Abcam, ab75769) was used in western blot on mouse samples (fig 5b). Sci Rep (2016) ncbi |
| mouse monoclonal (P1H12) |
| Abcam MCAM antibody (Abcam, ab24577) was used in flow cytometry on human samples (fig s4). Stem Cell Reports (2015) ncbi |
| mouse monoclonal (P1H12) |
| Abcam MCAM antibody (Abcam, ab24577) was used in western blot on human samples . Cell Signal (2015) ncbi |
| mouse monoclonal (P1H12) |
| In order to characterize capabilities and potentials of extracellular vesicle (EV) array, Abcam MCAM antibody (Abcam, ab24577) was used in other on human samples (fig 2). J Extracell Vesicles (2015) ncbi |
BioLegend
| mouse monoclonal (P1H12) |
| BioLegend MCAM antibody (Biolegend, 361006) was used in flow cytometry on human samples (fig s2). Sci Rep (2021) ncbi |
| mouse monoclonal (P1H12) |
| BioLegend MCAM antibody (BioLegend, 361014) was used in flow cytometry on human samples at 1:100 (fig s1a). Nat Microbiol (2021) ncbi |
| mouse monoclonal (P1H12) |
| BioLegend MCAM antibody (BioLegend, 361008) was used in flow cytometry on human samples (fig 4d). Nat Commun (2021) ncbi |
| mouse monoclonal (P1H12) |
| BioLegend MCAM antibody (Biolegend, 361005) was used in flow cytometry on human samples at 1:25 (fig s2). Stem Cell Res Ther (2020) ncbi |
| mouse monoclonal (P1H12) |
| BioLegend MCAM antibody (Biolegend, 361006) was used in flow cytometry on human samples (fig 1c). Stem Cell Res Ther (2019) ncbi |
| mouse monoclonal (SHM-57) |
| BioLegend MCAM antibody (Biolegend, 342008) was used in flow cytometry on human samples (fig 1b). Front Physiol (2016) ncbi |
| mouse monoclonal (P1H12) |
| BioLegend MCAM antibody (Bio-Legend, 361008) was used in flow cytometry on human samples (fig 1). J Clin Invest (2015) ncbi |
| mouse monoclonal (SHM-57) |
| In order to isolate and characterize endothelial cells and microparticles obtained directly from the site of cardiac damage, BioLegend MCAM antibody (Biolegend, 342008) was used in flow cytometry on human samples . Thromb Haemost (2015) ncbi |
| mouse monoclonal (SHM-57) |
| BioLegend MCAM antibody (BioLegend, SHM-57) was used in flow cytometry on human samples at 0.5 ug/ml. Sci Rep (2014) ncbi |
| mouse monoclonal (SHM-57) | In order to investigate the roles of SCRG1 and BST1 to the self-renewal, migration, and osteogenic differentiation of human mesenchymal stem cells, BioLegend MCAM antibody (Biolegend, 342003) was used . Sci Rep (2014) ncbi | |
| mouse monoclonal (SHM-57) |
| BioLegend MCAM antibody (Biolegend, 342003) was used in flow cytometry on human samples . PLoS ONE (2013) ncbi |
Bio-Rad
| mouse monoclonal (OJ79c) |
| Bio-Rad MCAM antibody (Bio-Rad, MCA2141F) was used in flow cytometry on human samples at 1:100 (fig 1h). elife (2020) ncbi |
| mouse monoclonal (OJ79c) |
| Bio-Rad MCAM antibody (Bio Rad, RRID:AB_324069) was used in flow cytometry on human samples (fig 1s1). elife (2019) ncbi |
| mouse monoclonal (OJ79c) |
| Bio-Rad MCAM antibody (AbD Serotec-BioRad, MCA2141A647) was used in flow cytometry on domestic horse samples (fig 2f). Stem Cell Res Ther (2017) ncbi |
| mouse monoclonal (OJ79c) |
| Bio-Rad MCAM antibody (Serotec, MCA2141F) was used in flow cytometry on human samples . J Vis Exp (2014) ncbi |
| mouse monoclonal (OJ79c) |
| Bio-Rad MCAM antibody (Serotec, MCA2141FT) was used in flow cytometry on human samples at 1:100. J Vis Exp (2014) ncbi |
| mouse monoclonal (OJ79c) |
| In order to identify the mesenchymal markers on human adipose stem/progenitor cells, Bio-Rad MCAM antibody (Serotec, MCA2141F) was used in immunohistochemistry - frozen section on human samples at 1:20. Cytometry A (2013) ncbi |
Invitrogen
| mouse monoclonal (P1H12) |
| Invitrogen MCAM antibody (Thermofisher, P1H12) was used in immunocytochemistry on mouse samples (fig 5s2c). elife (2021) ncbi |
| mouse monoclonal (P1H12) |
| Invitrogen MCAM antibody (eBiosciences, P1H12) was used in flow cytometry on mouse samples (fig s1a). EBioMedicine (2018) ncbi |
| mouse monoclonal (OJ79c) |
| In order to ask if the menstrual cycle stage and the location in the endometrial bilayer affects endometrial mesenchymal stem-like cells, Invitrogen MCAM antibody (Thermo Fisher, OJ79c) was used in flow cytometry on human samples at 1,000 ug/ml (fig 2). Exp Cell Res (2017) ncbi |
| mouse monoclonal (P1H12) |
| Invitrogen MCAM antibody (eBioscience, 14-1469) was used in flow cytometry on cat samples at 1:25 (tbl 3). Cell Reprogram (2015) ncbi |
Santa Cruz Biotechnology
| mouse monoclonal (P1H12) |
| Santa Cruz Biotechnology MCAM antibody (Santa Cruz, sc-18837) was used in flow cytometry on human samples (fig 4) and in western blot on human samples (fig 1). Sci Rep (2016) ncbi |
| mouse monoclonal (A-9) |
| In order to find predictive markers for patients that will develop gastrointestinal graft-versus-host-disease, Santa Cruz Biotechnology MCAM antibody (SantaCruz, sc-374556) was used in immunohistochemistry - paraffin section on human samples (fig 3a). JCI Insight (2016) ncbi |
| mouse monoclonal (P1H12) |
| Santa Cruz Biotechnology MCAM antibody (Santa Cruz, sc-18837) was used in immunocytochemistry on human samples at 1:200 (fig 4). Nat Commun (2016) ncbi |
| mouse monoclonal (P1H12) |
| In order to identify the cell surface markers in synovial mesenchymal stem cells, Santa Cruz Biotechnology MCAM antibody (Santa Cruz, sc-18837) was used in flow cytometry on human samples . Cytometry A (2015) ncbi |
| mouse monoclonal (P1H12) |
| Santa Cruz Biotechnology MCAM antibody (Santa Cruz, sc-18837) was used in western blot on mouse samples . J Biol Chem (2015) ncbi |
Miltenyi Biotec
| mouse monoclonal (541-10B2) |
| In order to identify the mesenchymal markers on human adipose stem/progenitor cells, Miltenyi Biotec MCAM antibody (Miltenyi, 130-092-852) was used in flow cytometry on human samples . Cytometry A (2013) ncbi |
Biocytex
| Biocytex MCAM antibody (Biocytex, 5050-PE100T) was used in flow cytometry on human samples (fig 1). Transl Psychiatry (2016) ncbi | |
| mouse monoclonal |
| Biocytex MCAM antibody (BioCytex SARL, 5050-F100T) was used in flow cytometry on human samples (tbl 1). Sci Rep (2016) ncbi |
| mouse monoclonal |
| Biocytex MCAM antibody (Biocytex, 5050-F100T) was used in flow cytometry on human samples (fig 1). Stem Cells Int (2016) ncbi |
Beckman Coulter
| mouse monoclonal (TEA 1/34) |
| Beckman Coulter MCAM antibody (Beckman Coulter, A22364) was used in flow cytometry on human samples (fig 3). Stem Cell Res Ther (2016) ncbi |
BD Biosciences
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD, P1H12) was used in flow cytometry on human samples (fig s3b). Front Immunol (2021) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD, 562136) was used in flow cytometry on human samples (fig 3b). J Clin Invest (2019) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Pharmingen, P1H12) was used in flow cytometry on human samples (fig 3a). Sci Rep (2018) ncbi |
| mouse monoclonal (P1H12) |
| In order to find predictive markers for patients that will develop gastrointestinal graft-versus-host-disease, BD Biosciences MCAM antibody (BD Biosciences, P1H12) was used in flow cytometry on human samples (fig 1b). JCI Insight (2016) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Biosciences, 550315) was used in flow cytometry on human samples (fig 2). Sci Rep (2016) ncbi |
| mouse monoclonal (P1H12) |
| In order to find cell-surface markers specific to human neutrophils, BD Biosciences MCAM antibody (BD, 550315) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi |
| mouse monoclonal (P1H12) |
| In order to optimize conditions to promote the proliferation of multipotent cardiovascular progenitor cells, BD Biosciences MCAM antibody (BD, 550315) was used in flow cytometry on human samples at 1:20 (fig s5). Nat Biotechnol (2015) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Biosciences, 560846) was used in flow cytometry on human samples (fig 2). J Endod (2015) ncbi |
| mouse monoclonal (P1H12) |
| In order to discuss methods and reagents used to examine endothelial cells by flow cytometry, BD Biosciences MCAM antibody (BD, P1H12) was used in flow cytometry on human samples . Rev Bras Hematol Hemoter (2015) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Biosciences, 560846) was used in flow cytometry on human samples (fig s5). Stem Cell Reports (2015) ncbi |
| mouse monoclonal (P1H12) |
| In order to report a protocol using a non-integrating Sendai virus vector for transduction of Yamanaka factors into urine cells collected from patients with muscular dystrophy, BD Biosciences MCAM antibody (BD Biosciences, 561013) was used in immunocytochemistry on human samples . J Vis Exp (2015) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD, 550315) was used in flow cytometry on human samples at 1:50. PLoS ONE (2014) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Biosciences, 562135) was used in flow cytometry on human samples . Am J Pathol (2015) ncbi |
| mouse monoclonal (P1H12) |
| In order to isolate and characterize endothelial cells and microparticles obtained directly from the site of cardiac damage, BD Biosciences MCAM antibody (BD, 562135) was used in flow cytometry on human samples . Thromb Haemost (2015) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Biosciences, 560846) was used in flow cytometry on human samples . Tissue Eng Part A (2015) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD, 550315) was used in flow cytometry on human samples . F1000Res (2014) ncbi |
| mouse monoclonal (P1H12) |
| In order to evaluate the osteogenic capacity of bone marrow mesenchymal stream cells and gingiva-derived mesenchymal stromal cells, BD Biosciences MCAM antibody (BD, 560846) was used in flow cytometry on human samples at 1:100. Biomed Mater (2014) ncbi |
| mouse monoclonal (P1H12) |
| In order to characterize VLA-4 blockade and promotion of differential routes into human CNS involving MCAM-adhesion of TH17 cells and PSGL-1 rolling of T cells, BD Biosciences MCAM antibody (BD, P1H12) was used in flow cytometry on human samples (fig 7). J Exp Med (2014) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD Pharmingen, 560846) was used in flow cytometry on human samples . Stem Cells Dev (2015) ncbi |
| mouse monoclonal (P1H12) |
| BD Biosciences MCAM antibody (BD, 550315) was used in flow cytometry on human samples . Cell Tissue Res (2014) ncbi |
Leica Biosystems
| monoclonal |
| In order to characterize human tracheal basal cells, Leica Biosystems MCAM antibody (Novocastra, NCL-CD146) was used in immunohistochemistry on human samples . Respir Res (2014) ncbi |
Articles Reviewed
- Ducret M, Fabre H, Degoul O, Atzeni G, McGuckin C, Forraz N, et al. Immunophenotyping Reveals the Diversity of Human Dental Pulp Mesenchymal Stromal Cells In vivo and Their Evolution upon In vitro Amplification. Front Physiol. 2016;7:512 pubmed
- Camilleri E, Gustafson M, Dudakovic A, Riester S, Garces C, Paradise C, et al. Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production. Stem Cell Res Ther. 2016;7:107 pubmed publisher
- Van de Laar E, Clifford M, Hasenoeder S, Kim B, Wang D, Lee S, et al. Cell surface marker profiling of human tracheal basal cells reveals distinct subpopulations, identifies MST1/MSP as a mitogenic signal, and identifies new biomarkers for lung squamous cell carcinomas. Respir Res. 2014;15:160 pubmed publisher
- Guerrero J, Oliveira H, Catros S, Siadous R, Derkaoui S, Bareille R, et al. The use of total human bone marrow fraction in a direct three-dimensional expansion approach for bone tissue engineering applications: focus on angiogenesis and osteogenesis. Tissue Eng Part A. 2015;21:861-74 pubmed publisher
articles
contribute
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
related gene review
questions and comments
