This is a Validated Antibody Database (VAD) review about human MBNL1, based on 10 published articles (read how Labome selects the articles), using MBNL1 antibody in all methods. It is aimed to help Labome visitors find the most suited MBNL1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MBNL1 synonym: EXP; MBNL; muscleblind-like protein 1; muscleblind-like; triplet-expansion RNA-binding protein

Abcam
rabbit monoclonal (EPR4565)
  • western blot; human; loading ...; fig 5b
Abcam MBNL1 antibody (Abcam, ab108519) was used in western blot on human samples (fig 5b). Nucleic Acids Res (2018) ncbi
rabbit monoclonal (EPR4565)
  • immunohistochemistry - paraffin section; human; 1:500; fig 8
In order to study recapitulation of SCA7 pathology and promotion of accumulation of the FUS/TLS and MBNL1 RNA-binding proteins by lentiviral vector-mediated overexpression of mutant ataxin-7, Abcam MBNL1 antibody (Abcam, ab108519) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 8). Mol Neurodegener (2016) ncbi
rabbit monoclonal (EPR4565)
  • western blot; mouse; 1:1000; fig 4
Abcam MBNL1 antibody (Abcam, ab108519) was used in western blot on mouse samples at 1:1000 (fig 4). Int J Mol Sci (2016) ncbi
rabbit monoclonal (EPR4565)
  • western blot; human; 1:1000
Abcam MBNL1 antibody (Abcam, ab108519) was used in western blot on human samples at 1:1000. Am J Pathol (2014) ncbi
LifeSpan Biosciences
mouse monoclonal (3E7)
  • western blot; human; loading ...; fig 5c
LifeSpan Biosciences MBNL1 antibody (LifeSpan Biosciences, LS-B4372) was used in western blot on human samples (fig 5c). elife (2016) ncbi
mouse monoclonal (3E7)
  • western blot; human; 1:1000; loading ...; fig s3a
In order to elucidate how CELF1 governs the epithelial-to-mesenchymal transition, LifeSpan Biosciences MBNL1 antibody (LifeSpan Biosciences, LS-B4372) was used in western blot on human samples at 1:1000 (fig s3a). Nat Commun (2016) ncbi
mouse monoclonal (3E7)
  • western blot; human; fig 1a
LifeSpan Biosciences MBNL1 antibody (LifeSpan BioSciences, LS-B4372) was used in western blot on human samples (fig 1a). Sci Rep (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3A4)
  • immunohistochemistry - paraffin section; human; 1:500; fig 8
  • immunohistochemistry - frozen section; mouse; 1:500; fig 7
  • western blot; mouse; 1:1000; fig 7
In order to study recapitulation of SCA7 pathology and promotion of accumulation of the FUS/TLS and MBNL1 RNA-binding proteins by lentiviral vector-mediated overexpression of mutant ataxin-7, Santa Cruz Biotechnology MBNL1 antibody (Santa Cruz, sc-47740) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 8), in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 7) and in western blot on mouse samples at 1:1000 (fig 7). Mol Neurodegener (2016) ncbi
mouse monoclonal (3A4)
  • immunocytochemistry; human; 1:100; fig 4
Santa Cruz Biotechnology MBNL1 antibody (Santa Cruz, sc-47740) was used in immunocytochemistry on human samples at 1:100 (fig 4). J Biol Chem (2015) ncbi
Abnova
mouse polyclonal
  • western blot; human; 1:300; fig 1
Abnova MBNL1 antibody (Abnova, H00004154) was used in western blot on human samples at 1:300 (fig 1). PLoS Genet (2016) ncbi
MilliporeSigma
mouse monoclonal (HL 1822)
  • western blot; human; loading ...; fig S1
MilliporeSigma MBNL1 antibody (Sigma-Aldrich, M3320) was used in western blot on human samples (fig S1). Nucleic Acids Res (2017) ncbi
Articles Reviewed
  1. Liu H, Lorenzini P, Zhang F, Xu S, Wong M, Zheng J, et al. Alternative splicing analysis in human monocytes and macrophages reveals MBNL1 as major regulator. Nucleic Acids Res. 2018;46:6069-6086 pubmed publisher
  2. Wongpalee S, Vashisht A, Sharma S, Chui D, Wohlschlegel J, Black D. Large-scale remodeling of a repressed exon ribonucleoprotein to an exon definition complex active for splicing. elife. 2016;5: pubmed publisher
  3. Chaudhury A, Cheema S, Fachini J, Kongchan N, Lu G, Simon L, et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. Nat Commun. 2016;7:13362 pubmed publisher
  4. Giudice J, Xia Z, Li W, Cooper T. Neonatal cardiac dysfunction and transcriptome changes caused by the absence of Celf1. Sci Rep. 2016;6:35550 pubmed publisher
  5. Kralovicova J, Vorechovsky I. Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons. Nucleic Acids Res. 2017;45:417-434 pubmed publisher
  6. Alves S, Marais T, Biferi M, Furling D, Marinello M, El Hachimi K, et al. Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins. Mol Neurodegener. 2016;11:58 pubmed publisher
  7. Zhang B, Cai H, Wei X, Sun J, Lan X, Lei C, et al. miR-30-5p Regulates Muscle Differentiation and Alternative Splicing of Muscle-Related Genes by Targeting MBNL. Int J Mol Sci. 2016;17: pubmed publisher
  8. Bondy Chorney E, Crawford Parks T, Ravel Chapuis A, Klinck R, Rocheleau L, Pelchat M, et al. Staufen1 Regulates Multiple Alternative Splicing Events either Positively or Negatively in DM1 Indicating Its Role as a Disease Modifier. PLoS Genet. 2016;12:e1005827 pubmed publisher
  9. Du J, Aleff R, Soragni E, Kalari K, Nie J, Tang X, et al. RNA toxicity and missplicing in the common eye disease fuchs endothelial corneal dystrophy. J Biol Chem. 2015;290:5979-90 pubmed publisher
  10. Screen M, Jonson P, Raheem O, Palmio J, Laaksonen R, Lehtimaki T, et al. Abnormal splicing of NEDD4 in myotonic dystrophy type 2: possible link to statin adverse reactions. Am J Pathol. 2014;184:2322-32 pubmed publisher