This is a Validated Antibody Database (VAD) review about human MARK2, based on 5 published articles (read how Labome selects the articles), using MARK2 antibody in all methods. It is aimed to help Labome visitors find the most suited MARK2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MARK2 synonym: EMK-1; EMK1; PAR-1; Par-1b; Par1b; serine/threonine-protein kinase MARK2; ELKL motif kinase 1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog b; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; testicular tissue protein Li 117

Abcam
rabbit monoclonal (EPR8553)
  • western blot; mouse; 1:1000; loading ...; fig 5a
Abcam MARK2 antibody (Abcam, ab133724) was used in western blot on mouse samples at 1:1000 (fig 5a). Mol Neurobiol (2017) ncbi
Invitrogen
rabbit polyclonal
  • western blot; rat; 1:1000
In order to show that NMDA receptors activate MARK/Par1 via protein kinase A in primary hippocampal neurons, Invitrogen MARK2 antibody (Thermo Scientific, PA5-17495) was used in western blot on rat samples at 1:1000. PLoS ONE (2015) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl MARK2 antibody (Bethyl, A303-135A) was used in RNA immunoprecipitation on human samples and in immunocytochemistry on human samples at 2 ug/ml. Mol Cell (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; rat; 1:2000; loading ...; fig 3a
In order to explore how laminin2 in the basement membrane modulates the blood testis barrier dynamics during spermatogenesis, Proteintech Group MARK2 antibody (Proteintech, 15492-1-AP) was used in western blot on rat samples at 1:2000 (fig 3a). FASEB J (2017) ncbi
Cell Signaling Technology
rabbit polyclonal
  • proximity ligation assay; mouse; fig 3
Cell Signaling Technology MARK2 antibody (Cell signaling, 9118S) was used in proximity ligation assay on mouse samples (fig 3). Nat Med (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3c
Sigma-Aldrich MARK2 antibody (Sigma, HPA038790) was used in immunocytochemistry on mouse samples at 1:500 (fig 3c). Mol Neurobiol (2017) ncbi
Articles Reviewed
  1. Gao Y, Mruk D, Chen H, Lui W, Lee W, Cheng C. Regulation of the blood-testis barrier by a local axis in the testis: role of laminin ?2 in the basement membrane. FASEB J. 2017;31:584-597 pubmed publisher
  2. Chen S, Zhang Q, Bai B, Ouyang S, Bao Y, Li H, et al. MARK2/Par1b Insufficiency Attenuates DVL Gene Transcription via Histone Deacetylation in Lumbosacral Spina Bifida. Mol Neurobiol. 2017;54:6304-6316 pubmed publisher
  3. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  4. Dumont N, Wang Y, von Maltzahn J, Pasut A, Bentzinger C, Brun C, et al. Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division. Nat Med. 2015;21:1455-63 pubmed publisher
  5. Bernard L, Zhang H. MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism. PLoS ONE. 2015;10:e0124816 pubmed publisher