This is a Validated Antibody Database (VAD) review about human MARCKS, based on 14 published articles (read how Labome selects the articles), using MARCKS antibody in all methods. It is aimed to help Labome visitors find the most suited MARCKS antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MARCKS synonym: 80K-L; MACS; PKCSL; PRKCSL

Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 10a
Abcam MARCKS antibody (Abcam, ab51100) was used in western blot on mouse samples at 1:1000 (fig 10a). Int J Mol Med (2018) ncbi
domestic rabbit polyclonal
  • flow cytometry; rat; fig 4
Abcam MARCKS antibody (Abcam, ab32590) was used in flow cytometry on rat samples (fig 4). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (EP1446Y)
  • western blot; human; 1:1000; fig 1
In order to analyze regulation of cellular PIP2 levels and nuclear PIP2 localization by the effector domain of MARCKS which acts as a nuclear localization signal, Abcam MARCKS antibody (Abcam, Ab52616) was used in western blot on human samples at 1:1000 (fig 1). PLoS ONE (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (JK-8)
  • western blot; human; 1:500; loading ...; fig 3e
Santa Cruz Biotechnology MARCKS antibody (Santa Cruz, sc-100777) was used in western blot on human samples at 1:500 (fig 3e). Mol Med Rep (2017) ncbi
mouse monoclonal (JK-8)
  • western blot; mouse; 1:1000; fig s7
In order to describe a role for HMGB1 in Alzheimer's disease pathogenesis, Santa Cruz Biotechnology MARCKS antibody (Santa Cruz Biotechnology, sc-100777) was used in western blot on mouse samples at 1:1000 (fig s7). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D88D11)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1b
  • immunocytochemistry; human; loading ...; fig 1c
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology MARCKS antibody (Cell signaling, 5607) was used in immunohistochemistry - paraffin section on human samples (fig 1b), in immunocytochemistry on human samples (fig 1c) and in western blot on human samples (fig 1d). J Cancer Res Clin Oncol (2020) ncbi
domestic rabbit monoclonal (D13E4)
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology MARCKS antibody (Cell signaling, 8722) was used in western blot on human samples (fig 1d). J Cancer Res Clin Oncol (2020) ncbi
domestic rabbit monoclonal (D13D2)
  • western blot; human; 1:1000; loading ...; fig 5m
Cell Signaling Technology MARCKS antibody (Cell Signaling, 11992) was used in western blot on human samples at 1:1000 (fig 5m). Cancer Res (2018) ncbi
domestic rabbit monoclonal (D88D11)
  • western blot; human; 1:1000; loading ...; fig 5m
Cell Signaling Technology MARCKS antibody (Cell Signaling, 5607) was used in western blot on human samples at 1:1000 (fig 5m). Cancer Res (2018) ncbi
domestic rabbit polyclonal
  • flow cytometry; human; 1:50; loading ...; fig 2a
In order to evaluate the methodology of microsphere cytometry for studying cellular microenvironment, Cell Signaling Technology MARCKS antibody (Cell Signaling, 2741) was used in flow cytometry on human samples at 1:50 (fig 2a). Integr Biol (Camb) (2017) ncbi
domestic rabbit monoclonal (D13E4)
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology MARCKS antibody (Cell Signaling, 8722) was used in western blot on human samples (fig 1b). FASEB J (2017) ncbi
domestic rabbit polyclonal
  • western blot; rat; fig 3
Cell Signaling Technology MARCKS antibody (Cell signaling, 2741) was used in western blot on rat samples (fig 3). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D88D11)
  • western blot; human; fig 6
Cell Signaling Technology MARCKS antibody (Cell Signaling Tech, 5607) was used in western blot on human samples (fig 6). PLoS Pathog (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 6
  • western blot; human; fig 6
Cell Signaling Technology MARCKS antibody (Cell Signaling Tech, 2741) was used in immunocytochemistry on human samples (fig 6) and in western blot on human samples (fig 6). PLoS Pathog (2016) ncbi
domestic rabbit monoclonal (D88D11)
  • immunocytochemistry; rat; fig 5b
  • immunocytochemistry; human; fig 4a
In order to study MARCKS signaling and how it expresses endothelial cell proliferation and vascular smooth muscle through a KIS-, p27kip1- dependent mechanism, Cell Signaling Technology MARCKS antibody (Cell Signaling Technology, 5607) was used in immunocytochemistry on rat samples (fig 5b) and in immunocytochemistry on human samples (fig 4a). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D88D11)
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Cell Signaling Technology MARCKS antibody (Cell Signaling, 5607S) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 1). J Am Heart Assoc (2015) ncbi
EMD Millipore
mouse monoclonal (2F12)
  • immunoprecipitation; human
  • western blot; human; fig 4
EMD Millipore MARCKS antibody (Millipore, 2F12) was used in immunoprecipitation on human samples and in western blot on human samples (fig 4). Am J Physiol Lung Cell Mol Physiol (2013) ncbi
Articles Reviewed
  1. Wenzel T, Buch T, Urban N, Weirauch U, Schierle K, Aigner A, et al. Restoration of MARCKS enhances chemosensitivity in cancer. J Cancer Res Clin Oncol. 2020;146:843-858 pubmed publisher
  2. Song H, Li X, Liu Y, Lu W, Cui Z, Zhou L, et al. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS. Int J Mol Med. 2018;42:193-207 pubmed publisher
  3. Jung Y, Cackowski F, Yumoto K, Decker A, Wang J, Kim J, et al. CXCL12γ Promotes Metastatic Castration-Resistant Prostate Cancer by Inducing Cancer Stem Cell and Neuroendocrine Phenotypes. Cancer Res. 2018;78:2026-2039 pubmed publisher
  4. Ertsås H, Nolan G, Labarge M, Lorens J. Microsphere cytometry to interrogate microenvironment-dependent cell signaling. Integr Biol (Camb). 2017;9:123-134 pubmed publisher
  5. Liu H, Su P, Zhi L, Zhao K. miR?34c?3p acts as a tumor suppressor gene in osteosarcoma by targeting MARCKS. Mol Med Rep. 2017;15:1204-1210 pubmed publisher
  6. Santo Domingo J, Chareyron I, Dayon L, Núñez Galindo A, Cominetti O, Pilar Giner Giménez M, et al. Coordinated activation of mitochondrial respiration and exocytosis mediated by PKC signaling in pancreatic ? cells. FASEB J. 2017;31:1028-1045 pubmed publisher
  7. Fujita K, Motoki K, Tagawa K, Chen X, Hama H, Nakajima K, et al. HMGB1, a pathogenic molecule that induces neurite degeneration via TLR4-MARCKS, is a potential therapeutic target for Alzheimer's disease. Sci Rep. 2016;6:31895 pubmed publisher
  8. Kim J, Hong S, Park C, Park J, Choi S, Woo S, et al. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction. PLoS ONE. 2016;11:e0158067 pubmed publisher
  9. Qvit N, Joshi A, Cunningham A, Ferreira J, Mochly Rosen D. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death. J Biol Chem. 2016;291:13608-21 pubmed publisher
  10. Cornick S, Moreau F, Chadee K. Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin. PLoS Pathog. 2016;12:e1005579 pubmed publisher
  11. Yu D, Makkar G, Dong T, Strickland D, Sarkar R, Monahan T. MARCKS Signaling Differentially Regulates Vascular Smooth Muscle and Endothelial Cell Proliferation through a KIS-, p27kip1- Dependent Mechanism. PLoS ONE. 2015;10:e0141397 pubmed publisher
  12. Rohrbach T, Shah N, Jackson W, Feeney E, Scanlon S, Gish R, et al. The Effector Domain of MARCKS Is a Nuclear Localization Signal that Regulates Cellular PIP2 Levels and Nuclear PIP2 Localization. PLoS ONE. 2015;10:e0140870 pubmed publisher
  13. Yu D, Makkar G, Strickland D, Blanpied T, Stumpo D, Blackshear P, et al. Myristoylated Alanine-Rich Protein Kinase Substrate (MARCKS) Regulates Small GTPase Rac1 and Cdc42 Activity and Is a Critical Mediator of Vascular Smooth Muscle Cell Migration in Intimal Hyperplasia Formation. J Am Heart Assoc. 2015;4:e002255 pubmed publisher
  14. Fang S, Crews A, Chen W, Park J, Yin Q, Ren X, et al. MARCKS and HSP70 interactions regulate mucin secretion by human airway epithelial cells in vitro. Am J Physiol Lung Cell Mol Physiol. 2013;304:L511-8 pubmed publisher