This is a Validated Antibody Database (VAD) review about human LGR5, based on 24 published articles (read how Labome selects the articles), using LGR5 antibody in all methods. It is aimed to help Labome visitors find the most suited LGR5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
LGR5 synonym: FEX; GPR49; GPR67; GRP49; HG38

Abcam
mouse monoclonal (OTI2A2)
  • immunohistochemistry - paraffin section; human; fig 8d
  • western blot; human; fig 8e
Abcam LGR5 antibody (Abcam, ab273092) was used in immunohistochemistry - paraffin section on human samples (fig 8d) and in western blot on human samples (fig 8e). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • immunohistochemistry; mouse; loading ...; fig 1e
  • western blot; mouse; 1:1000; loading ...; fig 1c
Abcam LGR5 antibody (Abcam, AB75850) was used in immunohistochemistry on mouse samples (fig 1e) and in western blot on mouse samples at 1:1000 (fig 1c). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • immunohistochemistry - paraffin section; human; loading ...; fig 3a
  • immunocytochemistry; human; loading ...; fig 3b
  • western blot; human; loading ...; fig 4a, s2a
Abcam LGR5 antibody (Abcam, ab199335) was used in immunohistochemistry - paraffin section on human samples (fig 3a), in immunocytochemistry on human samples (fig 3b) and in western blot on human samples (fig 4a, s2a). BMC Cancer (2019) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1a
Abcam LGR5 antibody (Abcam, ab75850) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1a). Gastric Cancer (2018) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • flow cytometry; mouse; loading ...; fig st2
In order to identify a role for Sca-1 in mouse gastric cancer, Abcam LGR5 antibody (Abcam, ab75850) was used in flow cytometry on mouse samples (fig st2). Stem Cells (2016) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • immunohistochemistry - paraffin section; mouse; fig 1
  • western blot; mouse; fig 3
  • immunohistochemistry - paraffin section; human; fig s12
Abcam LGR5 antibody (Abcam, EPR3065Y) was used in immunohistochemistry - paraffin section on mouse samples (fig 1), in western blot on mouse samples (fig 3) and in immunohistochemistry - paraffin section on human samples (fig s12). Sci Rep (2015) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • western blot; human; loading ...; fig 2c
Abcam LGR5 antibody (Abcam, ab75850) was used in western blot on human samples (fig 2c). Br J Cancer (2015) ncbi
domestic rabbit monoclonal (EPR3065Y)
  • immunohistochemistry - paraffin section; rat; 1:250
Abcam LGR5 antibody (Abcam, ab75850) was used in immunohistochemistry - paraffin section on rat samples at 1:250. BMC Cancer (2013) ncbi
OriGene
mouse monoclonal (OTI2A2)
  • flow cytometry; mouse; loading ...; fig 4e
OriGene LGR5 antibody (OriGene, TA400002) was used in flow cytometry on mouse samples (fig 4e). iScience (2021) ncbi
mouse monoclonal (OTI2A2)
  • immunohistochemistry; human; loading ...; fig 1d
  • western blot; human; loading ...; fig 1a
OriGene LGR5 antibody (OriGene Technologies, TA503316) was used in immunohistochemistry on human samples (fig 1d) and in western blot on human samples (fig 1a). Biol Open (2019) ncbi
mouse monoclonal (OTI2A2)
  • flow cytometry; human; fig 2b
OriGene LGR5 antibody (Acris Antibodies, TA400001) was used in flow cytometry on human samples (fig 2b). Oncogene (2018) ncbi
mouse monoclonal (OTI2A2)
  • immunocytochemistry; human; 1:400; fig 9
OriGene LGR5 antibody (OriGene, TA503316) was used in immunocytochemistry on human samples at 1:400 (fig 9). PLoS ONE (2016) ncbi
mouse monoclonal (OTI2A2)
  • immunocytochemistry; human; fig s1b
  • immunohistochemistry; human; loading ...; fig s5
OriGene LGR5 antibody (OriGene Tech, TA503316) was used in immunocytochemistry on human samples (fig s1b) and in immunohistochemistry on human samples (fig s5). Nat Biotechnol (2016) ncbi
mouse monoclonal (OTI2A2)
  • western blot; hamsters; 1:2000; loading ...; fig 1
  • western blot; African green monkey; 1:2000; loading ...; fig 1
  • western blot; human; 1:2000; loading ...; fig 1
  • western blot; mouse; 1:2000; loading ...; fig 1
OriGene LGR5 antibody (OriGene, TA503316) was used in western blot on hamsters samples at 1:2000 (fig 1), in western blot on African green monkey samples at 1:2000 (fig 1), in western blot on human samples at 1:2000 (fig 1) and in western blot on mouse samples at 1:2000 (fig 1). Biomed Rep (2016) ncbi
mouse monoclonal (OTI2A2)
  • immunohistochemistry - paraffin section; human; 1:300; fig s2
OriGene LGR5 antibody (Origene, TA503316) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig s2). J Clin Endocrinol Metab (2015) ncbi
mouse monoclonal (OTI2A2)
  • western blot; human; 1:1000; fig 2
OriGene LGR5 antibody (Origene, TA503316) was used in western blot on human samples at 1:1000 (fig 2). Oncotarget (2015) ncbi
mouse monoclonal (OTI2A2)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 5
  • western blot; human; 1:500; loading ...; fig 3d
OriGene LGR5 antibody (Origene, TA503316) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 5) and in western blot on human samples at 1:500 (fig 3d). J Cancer Res Clin Oncol (2015) ncbi
mouse monoclonal (OTI2A2)
  • western blot; human; 1:500
OriGene LGR5 antibody (OriGene, TA503316) was used in western blot on human samples at 1:500. Cell Signal (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3c
Invitrogen LGR5 antibody (Thermo Fisher Scientific, PA5-49691) was used in western blot on mouse samples at 1:1000 (fig 3c). JCI Insight (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3a
Invitrogen LGR5 antibody (ThermoFisher Scientific, PA5-49691) was used in western blot on human samples (fig 3a). Mol Cancer (2020) ncbi
Novus Biologicals
domestic rabbit polyclonal (2G10)
Novus Biologicals LGR5 antibody (novus Biologicals, NBP128904) was used . J Clin Endocrinol Metab (2015) ncbi
Miltenyi Biotec
rat monoclonal (DA03-22H2.8)
  • flow cytometry; human; loading ...; fig 3d
Miltenyi Biotec LGR5 antibody (Miltenyi Biotec GmbH, 130-100-856) was used in flow cytometry on human samples (fig 3d). EBioMedicine (2017) ncbi
BD Biosciences
rat monoclonal (8F2)
  • flow cytometry; human; loading ...
BD Biosciences LGR5 antibody (BD Biosciences, 562903) was used in flow cytometry on human samples . Adv Sci (Weinh) (2021) ncbi
rat monoclonal (4D11F8)
  • flow cytometry; human; loading ...; fig 2c
BD Biosciences LGR5 antibody (Becton Dickinson, 4D11F8) was used in flow cytometry on human samples (fig 2c). Br J Cancer (2015) ncbi
rat monoclonal (8F2)
  • flow cytometry; human; fig 4
BD Biosciences LGR5 antibody (BD Biosciences, 562903) was used in flow cytometry on human samples (fig 4). Oncotarget (2015) ncbi
rat monoclonal (4D11F8)
  • immunocytochemistry; human
BD Biosciences LGR5 antibody (BD Pharmingen, 4D11F8) was used in immunocytochemistry on human samples . J Immunol (2014) ncbi
Articles Reviewed
  1. Zhu H, Su Z, Ning J, Zhou L, Tan L, Sayed S, et al. Transmembrane protein 97 exhibits oncogenic properties via enhancing LRP6-mediated Wnt signaling in breast cancer. Cell Death Dis. 2021;12:912 pubmed publisher
  2. Shin J, Jeong J, Choi J, Lim J, Dinesh R, Braverman J, et al. Dickkopf-2 regulates the stem cell marker LGR5 in colorectal cancer via HNF4α1. iScience. 2021;24:102411 pubmed publisher
  3. Wang H, Gong P, Chen T, Gao S, Wu Z, Wang X, et al. Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single-Cell Analysis of Transcriptome and Telomeres. Adv Sci (Weinh). 2021;8:2004320 pubmed publisher
  4. Russo A, Colina J, Moy J, Baligod S, Czarnecki A, Varughese P, et al. Silencing PTEN in the fallopian tube promotes enrichment of cancer stem cell-like function through loss of PAX2. Cell Death Dis. 2021;12:375 pubmed publisher
  5. Li W, Zhang N, Jin C, Long M, Rajabi H, Yasumizu Y, et al. MUC1-C drives stemness in progression of colitis to colorectal cancer. JCI Insight. 2020;5: pubmed publisher
  6. Pothuraju R, Rachagani S, Krishn S, Chaudhary S, Nimmakayala R, Siddiqui J, et al. Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance. Mol Cancer. 2020;19:37 pubmed publisher
  7. Piao L, Yang Z, Feng Y, Zhang C, Cui C, Xuan Y. LETM1 is a potential biomarker of prognosis in lung non-small cell carcinoma. BMC Cancer. 2019;19:898 pubmed publisher
  8. Vazquez Iglesias L, Barcia Castro L, Rodríguez Quiroga M, Páez de la Cadena M, Rodríguez Berrocal J, Cordero O. Surface expression marker profile in colon cancer cell lines and sphere-derived cells suggests complexity in CD26+ cancer stem cells subsets. Biol Open. 2019;8: pubmed publisher
  9. Solomon H, Dinowitz N, Pateras I, Cooks T, Shetzer Y, Molchadsky A, et al. Mutant p53 gain of function underlies high expression levels of colorectal cancer stem cells markers. Oncogene. 2018;37:1669-1684 pubmed publisher
  10. Walker R, Poleszczuk J, Mejia J, Lee J, Pimiento J, Malafa M, et al. Toward early detection of Helicobacter pylori-associated gastric cancer. Gastric Cancer. 2018;21:196-203 pubmed publisher
  11. Liang X, Yuan X, Yu J, Wu Y, Li K, Sun C, et al. Histone Chaperone ASF1A Predicts Poor Outcomes for Patients With Gastrointestinal Cancer and Drives Cancer Progression by Stimulating Transcription of β-Catenin Target Genes. EBioMedicine. 2017;21:104-116 pubmed publisher
  12. Wang B, Chen Q, Cao Y, Ma X, Yin C, Jia Y, et al. LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1. PLoS ONE. 2016;11:e0168904 pubmed publisher
  13. Chen H, Wei Z, Sun J, Bhattacharya A, Savage D, Serda R, et al. A recellularized human colon model identifies cancer driver genes. Nat Biotechnol. 2016;34:845-51 pubmed publisher
  14. Alizadeh Navaei R, Rafiei A, Abedian Kenari S, Asgarian Omran H, Valadan R, Hedayatizadeh Omran A. Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines. Biomed Rep. 2016;5:130-132 pubmed
  15. Park J, Park J, Park D, Kim D, Kim H. Stem Cells Antigen-1 Enriches for a Cancer Stem Cell-Like Subpopulation in Mouse Gastric Cancer. Stem Cells. 2016;34:1177-87 pubmed publisher
  16. Su X, Yan H, Huang Y, Yun H, Zeng B, Wang E, et al. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus. Sci Rep. 2015;5:18588 pubmed publisher
  17. Shaikh L, Zhou J, Teo A, Garg S, Neogi S, Figg N, et al. LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal. J Clin Endocrinol Metab. 2015;100:E836-44 pubmed publisher
  18. Sharon C, Baranwal S, Patel N, Rodriguez Agudo D, Pandak W, Majumdar A, et al. Inhibition of insulin-like growth factor receptor/AKT/mammalian target of rapamycin axis targets colorectal cancer stem cells by attenuating mevalonate-isoprenoid pathway in vitro and in vivo. Oncotarget. 2015;6:15332-47 pubmed
  19. Morgan R, Molnár E, Jones R, Collard T, Lane J, Greenhough A, et al. Nutrient stress alters the glycosylation status of LGR5 resulting in reduced protein stability and membrane localisation in colorectal tumour cells: implications for targeting cancer stem cells. Br J Cancer. 2015;112:714-9 pubmed publisher
  20. Jonchère B, Vétillard A, Toutain B, Lam D, Bernard A, Henry C, et al. Irinotecan treatment and senescence failure promote the emergence of more transformed and invasive cells that depend on anti-apoptotic Mcl-1. Oncotarget. 2015;6:409-26 pubmed
  21. Hsu H, Liu Y, Tseng K, Yang T, Yeh C, You J, et al. CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression. J Cancer Res Clin Oncol. 2015;141:11-21 pubmed publisher
  22. Hsu H, Liu Y, Tseng K, Tan B, Chen S, Chen H. LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/?-catenin signaling pathway in colorectal cancer cells. Cell Signal. 2014;26:2333-42 pubmed publisher
  23. Volonté A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, et al. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4. J Immunol. 2014;192:523-32 pubmed publisher
  24. Femia A, Dolara P, Salvadori M, Caderni G. Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early phases of 1,2-dimethylhydrazine-induced rat colon carcinogenesis: correlation with nuclear ?-catenin. BMC Cancer. 2013;13:48 pubmed publisher