This is a Validated Antibody Database (VAD) review about human LDLR, based on 33 published articles (read how Labome selects the articles), using LDLR antibody in all methods. It is aimed to help Labome visitors find the most suited LDLR antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
LDLR synonym: FH; FHC; FHCL1; LDLCQ2

Knockout validation
Abcam
domestic rabbit monoclonal (EP1553Y)
  • western blot knockout validation; human; 1:2000; loading ...; fig 4b
Abcam LDLR antibody (Abcam, ab52818) was used in western blot knockout validation on human samples at 1:2000 (fig 4b). PLoS Genet (2021) ncbi
Abcam
domestic rabbit monoclonal (EP1553Y)
  • western blot knockout validation; human; loading ...; fig e4e
Abcam LDLR antibody (Abcam, ab52818) was used in western blot knockout validation on human samples (fig e4e). Nature (2019) ncbi
Abcam
domestic rabbit monoclonal (EP1553Y)
  • immunohistochemistry knockout validation; mouse; 1:1000; fig 4
  • western blot knockout validation; mouse; 1:1000; fig 2
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 6
Abcam LDLR antibody (Novus Biologicals, EP1553Y) was used in immunohistochemistry knockout validation on mouse samples at 1:1000 (fig 4), in western blot knockout validation on mouse samples at 1:1000 (fig 2) and in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 6). J Lipid Res (2016) ncbi
Abcam
domestic rabbit monoclonal (EP1553Y)
  • western blot knockout validation; pigs ; fig 2
Abcam LDLR antibody (Abcam, EP1553Y) was used in western blot knockout validation on pigs samples (fig 2). PLoS ONE (2014) ncbi
Abcam
domestic rabbit monoclonal (EP1553Y)
  • western blot; mouse; 1:1000; fig 7a
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on mouse samples at 1:1000 (fig 7a). Cancer Res (2021) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; mouse; 1.4 ug/ml; loading ...; fig 1e
  • western blot; human; 1.4 ug/ml; loading ...; fig 1d
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on mouse samples at 1.4 ug/ml (fig 1e) and in western blot on human samples at 1.4 ug/ml (fig 1d). J Biol Chem (2021) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot knockout validation; human; 1:2000; loading ...; fig 4b
Abcam LDLR antibody (Abcam, ab52818) was used in western blot knockout validation on human samples at 1:2000 (fig 4b). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; human; 1:250; loading ...; fig 5g
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on human samples at 1:250 (fig 5g). Nat Metab (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 8e
Abcam LDLR antibody (Abcam, ab30532) was used in western blot on human samples (fig 8e). Toxicology (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5c
  • western blot; human; loading ...; fig 4b
Abcam LDLR antibody (Abcam, ab30532) was used in western blot on mouse samples (fig 5c) and in western blot on human samples (fig 4b). Front Pharmacol (2019) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; human; loading ...; fig 4g
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on human samples (fig 4g). Nature (2019) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot knockout validation; human; loading ...; fig e4e
Abcam LDLR antibody (Abcam, ab52818) was used in western blot knockout validation on human samples (fig e4e). Nature (2019) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; mouse; loading ...; fig 3a
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on mouse samples (fig 3a). Mol Metab (2018) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; mouse; loading ...; fig s5d
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on mouse samples (fig s5d). PLoS Genet (2017) ncbi
domestic rabbit monoclonal (EP1553Y)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 4d
  • western blot; human; loading ...; fig 4c
Abcam LDLR antibody (abcam, ab52818) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 4d) and in western blot on human samples (fig 4c). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1e
Abcam LDLR antibody (Abcam, ab30532) was used in western blot on mouse samples (fig 1e). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; human; 1:4000; loading ...; fig s1a
Abcam LDLR antibody (Abcam, EP1553Y) was used in western blot on human samples at 1:4000 (fig s1a). PLoS ONE (2017) ncbi
mouse monoclonal (1B10H10)
  • flow cytometry; human; loading ...; fig 3g
Abcam LDLR antibody (Abcam, ab204941) was used in flow cytometry on human samples (fig 3g). ACS Nano (2017) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; mouse; 1:1000; loading ...; fig 3
In order to assess the effects of statins on Trypanosoma cruzi infection-induced myocarditis, Abcam LDLR antibody (Abcam, AB52818) was used in western blot on mouse samples at 1:1000 (fig 3). Microbes Infect (2016) ncbi
domestic rabbit monoclonal (EP1553Y)
  • immunohistochemistry knockout validation; mouse; 1:1000; fig 4
  • western blot knockout validation; mouse; 1:1000; fig 2
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 6
Abcam LDLR antibody (Novus Biologicals, EP1553Y) was used in immunohistochemistry knockout validation on mouse samples at 1:1000 (fig 4), in western blot knockout validation on mouse samples at 1:1000 (fig 2) and in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 6). J Lipid Res (2016) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; human; 1:4000; fig 2
In order to examine the role of deubiquitylating enzymes in cholesterol metabolism, Abcam LDLR antibody (Epitomics, EP1553Y) was used in western blot on human samples at 1:4000 (fig 2). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; human; fig 3
Abcam LDLR antibody (abcam, ab52818) was used in western blot on human samples (fig 3). BMC Cancer (2015) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; mouse; loading ...; fig 7B
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on mouse samples (fig 7B). Sci Rep (2015) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot; human
Abcam LDLR antibody (Abcam, ab52818) was used in western blot on human samples . Sci Rep (2015) ncbi
domestic rabbit monoclonal (EP1553Y)
  • western blot knockout validation; pigs ; fig 2
Abcam LDLR antibody (Abcam, EP1553Y) was used in western blot knockout validation on pigs samples (fig 2). PLoS ONE (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C7)
  • western blot; human; 1:1000; loading ...; fig s2h
Santa Cruz Biotechnology LDLR antibody (Santa Cruz, sc-18823) was used in western blot on human samples at 1:1000 (fig s2h). Adv Sci (Weinh) (2022) ncbi
mouse monoclonal (C7)
  • western blot; human; loading ...; fig 6b
Santa Cruz Biotechnology LDLR antibody (Santa Cruz, sc-18823) was used in western blot on human samples (fig 6b). Biomedicines (2020) ncbi
mouse monoclonal (F-7)
  • immunocytochemistry; human; 1:100; loading ...; fig 1d
Santa Cruz Biotechnology LDLR antibody (Santa Cruz, SC373830) was used in immunocytochemistry on human samples at 1:100 (fig 1d). Viruses (2019) ncbi
mouse monoclonal (C7)
  • western blot; human; fig 1
Santa Cruz Biotechnology LDLR antibody (Santa Cruz, sc-18823) was used in western blot on human samples (fig 1). Cell Death Dis (2015) ncbi
mouse monoclonal (C7)
  • western blot; human; loading ...; fig 4a
Santa Cruz Biotechnology LDLR antibody (Santa Cruz, sc-18823) was used in western blot on human samples (fig 4a). Nat Med (2015) ncbi
mouse monoclonal (C7)
  • proximity ligation assay; human; loading ...; fig 13
  • immunocytochemistry; human; 1:20; loading ...; fig 2a
Santa Cruz Biotechnology LDLR antibody (Santa Cruz, C7) was used in proximity ligation assay on human samples (fig 13) and in immunocytochemistry on human samples at 1:20 (fig 2a). J Biol Chem (2015) ncbi
Invitrogen
domestic rabbit recombinant (SJ0197)
  • western blot; mouse; loading ...; fig s6a
Invitrogen LDLR antibody (Thermo Fisher, MA532075) was used in western blot on mouse samples (fig s6a). JCI Insight (2020) ncbi
R&D Systems
domestic goat polyclonal
  • western blot; human; 1:1000; fig 2b
R&D Systems LDLR antibody (R&D, AF2148) was used in western blot on human samples at 1:1000 (fig 2b). J Biol Chem (2021) ncbi
Abnova
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; fig 3
  • western blot; mouse; 1:1000; fig 2
In order to analyze how WASH- and CCC-mediated endosomal sorting of LDLr is needed for regular clearance of circulating LDL, Abnova LDLR antibody (Abnova, PAB8804) was used in immunocytochemistry on mouse samples at 1:200 (fig 3) and in western blot on mouse samples at 1:1000 (fig 2). Nat Commun (2016) ncbi
Sino Biological
domestic rabbit monoclonal (301)
  • flow cytometry; human; fig 1
Sino Biological LDLR antibody (SinoBiologicals, 10231-R3031-P) was used in flow cytometry on human samples (fig 1). PLoS ONE (2016) ncbi
Fitzgerald Industries
  • immunocytochemistry; human; fig 6g
Fitzgerald Industries LDLR antibody (Fitzgerald, 20R-LR002) was used in immunocytochemistry on human samples (fig 6g). EMBO Rep (2020) ncbi
  • western blot; human
Fitzgerald Industries LDLR antibody (Fitzgerald Industries, 20R-LR002) was used in western blot on human samples . J Virol (2014) ncbi
Articles Reviewed
  1. Zheng S, Lin J, Pang Z, Zhang H, Wang Y, Ma L, et al. Aberrant Cholesterol Metabolism and Wnt/β-Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer Growth. Adv Sci (Weinh). 2022;9:e2200750 pubmed publisher
  2. Susan Resiga D, Girard E, Essalmani R, Roubtsova A, Marcinkiewicz J, Derbali R, et al. Asialoglycoprotein receptor 1 is a novel PCSK9-independent ligand of liver LDLR cleaved by furin. J Biol Chem. 2021;297:101177 pubmed publisher
  3. Kemp S, Carpenter E, Steele N, Donahue K, Nwosu Z, Pacheco A, et al. Apolipoprotein E Promotes Immune Suppression in Pancreatic Cancer through NF-κB-Mediated Production of CXCL1. Cancer Res. 2021;81:4305-4318 pubmed publisher
  4. Muramatsu M, Osawa T, Miyamura Y, Nakagawa S, Tanaka T, Kodama T, et al. Loss of Down syndrome critical region-1 leads to cholesterol metabolic dysfunction that exaggerates hypercholesterolemia in ApoE-null background. J Biol Chem. 2021;296:100697 pubmed publisher
  5. Emmer B, Sherman E, Lascuna P, Graham S, Willer C, Ginsburg D. Genome-scale CRISPR screening for modifiers of cellular LDL uptake. PLoS Genet. 2021;17:e1009285 pubmed publisher
  6. Nakayama A, Albarrán Juárez J, Liang G, Roquid K, Iring A, Tonack S, et al. Disturbed flow-induced Gs-mediated signaling protects against endothelial inflammation and atherosclerosis. JCI Insight. 2020;5: pubmed publisher
  7. He B, Moreau R. R-α-Lipoic Acid and 4-Phenylbutyric Acid Have Distinct Hypolipidemic Mechanisms in Hepatic Cells. Biomedicines. 2020;8: pubmed publisher
  8. Aregger M, Lawson K, Billmann M, Costanzo M, Tong A, Chan K, et al. Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nat Metab. 2020;2:499-513 pubmed publisher
  9. Moessinger C, Nilsson I, Muhl L, Zeitelhofer M, Heller Sahlgren B, Skogsberg J, et al. VEGF-B signaling impairs endothelial glucose transcytosis by decreasing membrane cholesterol content. EMBO Rep. 2020;21:e49343 pubmed publisher
  10. Sa Ngiamsuntorn K, Thongsri P, Pewkliang Y, Wongkajornsilp A, Kongsomboonchoke P, Suthivanich P, et al. An Immortalized Hepatocyte-like Cell Line (imHC) Accommodated Complete Viral Lifecycle, Viral Persistence Form, cccDNA and Eventual Spreading of a Clinically-Isolated HBV. Viruses. 2019;11: pubmed publisher
  11. Zhang G, Zhou J, Huang W, Fang M, Yu L, Wang H, et al. Prenatal ethanol exposure-induced a low level of foetal blood cholesterol and its mechanism of IGF1-related placental cholesterol transport dysfunction. Toxicology. 2019;:152237 pubmed publisher
  12. Xu F, Xiao H, Liu R, Yang Y, Zhang M, Chen L, et al. Paeonol Ameliorates Glucose and Lipid Metabolism in Experimental Diabetes by Activating Akt. Front Pharmacol. 2019;10:261 pubmed publisher
  13. Jiang Y, Sun A, Zhao Y, Ying W, Sun H, Yang X, et al. Proteomics identifies new therapeutic targets of early-stage hepatocellular carcinoma. Nature. 2019;567:257-261 pubmed publisher
  14. Garcia Bermudez J, Baudrier L, Bayraktar E, Shen Y, La K, Guarecuco R, et al. Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death. Nature. 2019;567:118-122 pubmed publisher
  15. Zhu L, Shi J, Luu T, Neuman J, Trefts E, Yu S, et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab. 2018;8:106-116 pubmed publisher
  16. Rocchi A, Yamamoto S, Ting T, Fan Y, SADLEIR K, Wang Y, et al. A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. PLoS Genet. 2017;13:e1006962 pubmed publisher
  17. Gallagher E, Zelenko Z, Neel B, Antoniou I, Rajan L, Kase N, et al. Elevated tumor LDLR expression accelerates LDL cholesterol-mediated breast cancer growth in mouse models of hyperlipidemia. Oncogene. 2017;36:6462-6471 pubmed publisher
  18. Vienken H, Mabrouki N, Grabau K, Claas R, Rudowski A, Schömel N, et al. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype with enhanced lysosomal Ca2+ storage. Sci Rep. 2017;7:43575 pubmed publisher
  19. Cook E, Nelson J, Sorrentino V, Koenis D, Moeton M, Scheij S, et al. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE. 2017;12:e0172721 pubmed publisher
  20. Lara S, Alnasser F, Polo E, Garry D, Lo Giudice M, Hristov D, et al. Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles. ACS Nano. 2017;11:1884-1893 pubmed publisher
  21. Zhao D, Lizardo K, Cui M, Ambadipudi K, Lora J, Jelicks L, et al. Antagonistic effect of atorvastatin on high fat diet induced survival during acute Chagas disease. Microbes Infect. 2016;18:675-686 pubmed publisher
  22. Topchiy E, Cirstea M, Kong H, Boyd J, Wang Y, Russell J, et al. Lipopolysaccharide Is Cleared from the Circulation by Hepatocytes via the Low Density Lipoprotein Receptor. PLoS ONE. 2016;11:e0155030 pubmed publisher
  23. Tao W, Moore R, Meng Y, Smith E, Xu X. Endocytic adaptors Arh and Dab2 control homeostasis of circulatory cholesterol. J Lipid Res. 2016;57:809-17 pubmed publisher
  24. Bartuzi P, Billadeau D, Favier R, Rong S, Dekker D, Fedoseienko A, et al. CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Nat Commun. 2016;7:10961 pubmed publisher
  25. Nelson J, Cook E, Loregger A, Hoeksema M, Scheij S, Kovacevic I, et al. Deubiquitylase Inhibition Reveals Liver X Receptor-independent Transcriptional Regulation of the E3 Ubiquitin Ligase IDOL and Lipoprotein Uptake. J Biol Chem. 2016;291:4813-25 pubmed publisher
  26. Laezza C, D Alessandro A, Di Croce L, Picardi P, Ciaglia E, Pisanti S, et al. p53 regulates the mevalonate pathway in human glioblastoma multiforme. Cell Death Dis. 2015;6:e1909 pubmed publisher
  27. Goedeke L, Rotllan N, Canfrán Duque A, Aranda J, Ramírez C, Araldi E, et al. MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nat Med. 2015;21:1280-9 pubmed publisher
  28. Le Q, Blanchet M, Seidah N, Labonté P. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9. J Biol Chem. 2015;290:23385-400 pubmed publisher
  29. De Gonzalo Calvo D, López Vilaró L, Nasarre L, Pérez Olabarría M, Vázquez T, Escuin D, et al. Intratumor cholesteryl ester accumulation is associated with human breast cancer proliferation and aggressive potential: a molecular and clinicopathological study. BMC Cancer. 2015;15:460 pubmed publisher
  30. Zhong C, Sun W, Ma Y, Zhu H, Yang P, Wei H, et al. Microbiota prevents cholesterol loss from the body by regulating host gene expression in mice. Sci Rep. 2015;5:10512 pubmed publisher
  31. Stöhr R, Mavilio M, Marino A, Casagrande V, Kappel B, Möllmann J, et al. ITCH modulates SIRT6 and SREBP2 to influence lipid metabolism and atherosclerosis in ApoE null mice. Sci Rep. 2015;5:9023 pubmed publisher
  32. Davis B, Wang X, Rohret J, Struzynski J, Merricks E, Bellinger D, et al. Targeted disruption of LDLR causes hypercholesterolemia and atherosclerosis in Yucatan miniature pigs. PLoS ONE. 2014;9:e93457 pubmed publisher
  33. Prentoe J, Serre S, Ramírez S, Nicosia A, Gottwein J, Bukh J. Hypervariable region 1 deletion and required adaptive envelope mutations confer decreased dependency on scavenger receptor class B type I and low-density lipoprotein receptor for hepatitis C virus. J Virol. 2014;88:1725-39 pubmed publisher