This is a Validated Antibody Database (VAD) review about human LAT, based on 20 published articles (read how Labome selects the articles), using LAT antibody in all methods. It is aimed to help Labome visitors find the most suited LAT antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
LAT synonym: IMD52; LAT1; pp36; linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells, transmembrane adaptor; p36-38

BioLegend
mouse monoclonal (11B.12)
  • western blot; mouse; fig s1
BioLegend LAT antibody (BioLegend, 11B.12) was used in western blot on mouse samples (fig s1). J Clin Invest (2018) ncbi
LifeSpan Biosciences
mouse monoclonal (LAT-1D1)
  • western blot; mouse; loading ...; fig 6b
LifeSpan Biosciences LAT antibody (LifeSpan BioSciences, LS-C46104) was used in western blot on mouse samples (fig 6b). Mol Biol Cell (2017) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 8
  • western blot; human; 1:1000; fig 6
In order to research the drive of early signaling and vesicle dynamics during T-cell activation by Aurora A, Abcam LAT antibody (Abcam, ab4476) was used in western blot on mouse samples at 1:1000 (fig 8) and in western blot on human samples at 1:1000 (fig 6). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3d
In order to identify and characterize human ZAP-70 mutations associated with autoimmune disease, Abcam LAT antibody (Abcam, ab4476) was used in western blot on human samples (fig 3d). J Exp Med (2016) ncbi
Invitrogen
rabbit polyclonal
  • western blot; mouse; fig s5
In order to examine the quantitative and temporal requirements of Zap70's function in thymocyte development, Invitrogen LAT antibody (Life Technologies, 44224) was used in western blot on mouse samples (fig s5). Nat Immunol (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; mouse; loading ...; fig 3a
Cell Signaling Technology LAT antibody (Cell signaling technology, 3581S) was used in western blot on mouse samples (fig 3a). Cell Rep (2019) ncbi
rabbit polyclonal
  • western blot; human; fig 7c
Cell Signaling Technology LAT antibody (Cell signaling, 3584S) was used in western blot on human samples (fig 7c). Nat Commun (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig s7b
In order to demonstrate that PD-1 inhibits T cell function primarily by inactivating CD28 signaling, Cell Signaling Technology LAT antibody (Cell Signaling, 3581) was used in western blot on human samples (fig s7b). Science (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig s12
In order to develop a new multiplex dSTORM imaging strategy to localize epitopes on activated T-cells, Cell Signaling Technology LAT antibody (Cell signaling, 3584BF) was used in immunocytochemistry on human samples (fig s12). Mol Biol Cell (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 4
Cell Signaling Technology LAT antibody (Cell signaling, 3581S) was used in immunocytochemistry on human samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 3e
Cell Signaling Technology LAT antibody (Cell Signaling, 3584) was used in western blot on mouse samples (fig 3e). J Immunol (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:25; loading ...; fig 3g
  • western blot; mouse; loading ...; fig 3e
Cell Signaling Technology LAT antibody (Cell Signaling, 9166) was used in immunocytochemistry on mouse samples at 1:25 (fig 3g) and in western blot on mouse samples (fig 3e). J Immunol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Cell Signaling Technology LAT antibody (Cell Signaling, 3584) was used in western blot on mouse samples (fig 5). J Exp Med (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2
In order to investigate the control of LAT recruitment to the immune synapse and T-cell activation in vivo by IFT20, Cell Signaling Technology LAT antibody (Cell Signaling, 3584) was used in western blot on human samples (fig 2). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to study how conformational changes control T cell receptor association and activity of Zap70, Cell Signaling Technology LAT antibody (Cell Signaling Technology, 3584) was used in western blot on human samples (fig 4). Nat Immunol (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology LAT antibody (Cell Signaling Technology, 9166) was used in western blot on human samples . Mol Immunol (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; loading ...; fig 2d
In order to study the role of TMEM16F in limiting T cell responses in mice infected with virus, EMD Millipore LAT antibody (Millipore, 07-278) was used in western blot on mouse samples (fig 2d). J Exp Med (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to study how conformational changes control T cell receptor association and activity of Zap70, EMD Millipore LAT antibody (Millipore, 06-807) was used in western blot on human samples (fig 4). Nat Immunol (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:500; tbl 4
EMD Millipore LAT antibody (Millipore, 07-278) was used in western blot on human samples at 1:500 (tbl 4). Methods Mol Biol (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; tbl 4
EMD Millipore LAT antibody (Millipore, 06-807) was used in western blot on human samples at 1:1000 (tbl 4). Methods Mol Biol (2014) ncbi
mouse monoclonal (11B.12)
  • immunocytochemistry; human; 2 ug/ml
EMD Millipore LAT antibody (Millipore, 05-770) was used in immunocytochemistry on human samples at 2 ug/ml. Biochim Biophys Acta (2014) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore LAT antibody (Millipore, 06-807) was used in western blot on human samples . Genes Immun (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
EMD Millipore LAT antibody (Millipore, 06-807) was used in western blot on mouse samples at 1:1000. J Immunol Methods (2013) ncbi
Articles Reviewed
  1. Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
  2. Zhao X, Sankaran S, Yap J, Too C, Ho Z, Dolton G, et al. Nonstimulatory peptide-MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling. Nat Commun. 2018;9:2716 pubmed publisher
  3. Muro R, Nitta T, Nakano K, Okamura T, Takayanagi H, Suzuki H. γδTCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program. J Clin Invest. 2018;128:415-426 pubmed publisher
  4. Schwartz S, Cleyrat C, Olah M, Relich P, Phillips G, Hlavacek W, et al. Differential mast cell outcomes are sensitive to FcεRI-Syk binding kinetics. Mol Biol Cell. 2017;28:3397-3414 pubmed publisher
  5. Hui E, Cheung J, Zhu J, Su X, Taylor M, Wallweber H, et al. T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition. Science. 2017;355:1428-1433 pubmed publisher
  6. Hu Y, Kim J, He K, Wan Q, Kim J, Flach M, et al. Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. J Exp Med. 2016;213:2759-2772 pubmed
  7. Yi J, Manna A, Barr V, Hong J, Neuman K, Samelson L. madSTORM: a superresolution technique for large-scale multiplexing at single-molecule accuracy. Mol Biol Cell. 2016;27:3591-3600 pubmed
  8. Pageon S, Tabarin T, Yamamoto Y, Ma Y, Nicovich P, Bridgeman J, et al. Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. Proc Natl Acad Sci U S A. 2016;113:E5454-63 pubmed publisher
  9. Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
  10. Naik E, Dixit V. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCβ Kinase Activity. J Immunol. 2016;196:3438-51 pubmed publisher
  11. Hu H, Wang H, Xiao Y, Jin J, Chang J, Zou Q, et al. Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination. J Exp Med. 2016;213:399-414 pubmed publisher
  12. Chan A, Punwani D, Kadlecek T, Cowan M, Olson J, Mathes E, et al. A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70. J Exp Med. 2016;213:155-65 pubmed publisher
  13. Vivar O, Masi G, Carpier J, Magalhaes J, Galgano D, Pazour G, et al. IFT20 controls LAT recruitment to the immune synapse and T-cell activation in vivo. Proc Natl Acad Sci U S A. 2016;113:386-91 pubmed publisher
  14. Klammt C, Novotná L, Li D, Wolf M, Blount A, Zhang K, et al. T cell receptor dwell times control the kinase activity of Zap70. Nat Immunol. 2015;16:961-9 pubmed publisher
  15. Ebsen H, Lettau M, Kabelitz D, Janssen O. Subcellular localization and activation of ADAM proteases in the context of FasL shedding in T lymphocytes. Mol Immunol. 2015;65:416-28 pubmed publisher
  16. Cox S. Intracellular signaling of CTLs. Methods Mol Biol. 2014;1186:49-63 pubmed publisher
  17. Chakrabarti A, Witsenburg J, Sinzinger M, Richter M, Wallbrecher R, Cluitmans J, et al. Multivalent presentation of the cell-penetrating peptide nona-arginine on a linear scaffold strongly increases its membrane-perturbing capacity. Biochim Biophys Acta. 2014;1838:3097-106 pubmed publisher
  18. Kłossowicz M, Marek Bukowiec K, Arbulo Echevarria M, Scirka B, Majkowski M, Sikorski A, et al. Identification of functional, short-lived isoform of linker for activation of T cells (LAT). Genes Immun. 2014;15:449-56 pubmed publisher
  19. Au Yeung B, Melichar H, Ross J, Cheng D, Zikherman J, Shokat K, et al. Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development. Nat Immunol. 2014;15:687-94 pubmed publisher
  20. Kłossowicz M, Scirka B, Suchanek J, Marek Bukowiec K, Kisielow P, Aguado E, et al. Assessment of caspase mediated degradation of linker for activation of T cells (LAT) at a single cell level. J Immunol Methods. 2013;389:9-17 pubmed publisher