This is a Validated Antibody Database (VAD) review about human L1CAM, based on 21 published articles (read how Labome selects the articles), using L1CAM antibody in all methods. It is aimed to help Labome visitors find the most suited L1CAM antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
L1CAM synonym: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

Knockout validation
MilliporeSigma
mouse monoclonal (UJ127.11)
  • western blot knockout validation; human; fig 10
In order to elucidate the mechanisms by which L1CAM gene mutations result in neuronal defects, MilliporeSigma L1CAM antibody (Sigma-Aldrich, UJ127.11) was used in western blot knockout validation on human samples (fig 10). J Exp Med (2016) ncbi
Invitrogen
mouse monoclonal (UJ127)
  • immunocytochemistry; human; 1:200; loading ...; fig s2c
Invitrogen L1CAM antibody (Thermo Fisher, MA514140) was used in immunocytochemistry on human samples at 1:200 (fig s2c). Cell Rep Med (2022) ncbi
mouse monoclonal (eBio5G3 (5G3))
  • flow cytometry; human; fig 1
Invitrogen L1CAM antibody (eBioscience, eBio5G3) was used in flow cytometry on human samples (fig 1). Cancers (Basel) (2019) ncbi
mouse monoclonal (eBio5G3 (5G3))
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, Invitrogen L1CAM antibody (e- Bioscienc e, 12-1719-73) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (UJ127)
  • immunohistochemistry; human; 1:100
In order to compare the immunohistochemical and genetic profiles found in the three types of endometrial carcinomas, Invitrogen L1CAM antibody (Thermo Scientific, UJ127) was used in immunohistochemistry on human samples at 1:100. Gynecol Oncol (2015) ncbi
mouse monoclonal (eBio5G3 (5G3))
  • western blot; mouse; 1:1000
Invitrogen L1CAM antibody (eBioscience, 5G3) was used in western blot on mouse samples at 1:1000. FASEB J (2015) ncbi
mouse monoclonal (UJ127)
  • ELISA; human; 1 ug/ml; fig 1
In order to determine how using liposome-encapsulated siRNA to target L1 cell adhesion molecule expression suppresses prostate cancer bone metastasis and growth, Invitrogen L1CAM antibody (NeoMarker, UJ127) was used in ELISA on human samples at 1 ug/ml (fig 1). Oncotarget (2014) ncbi
mouse monoclonal (UJ127)
  • western blot; mouse; fig 1
In order to elucidate the association between L1 expression and motile processes involved in metastasis and development, Invitrogen L1CAM antibody (Neomarkers, UJ127) was used in western blot on mouse samples (fig 1). J Biol Chem (2004) ncbi
Abcam
mouse monoclonal (2C2)
  • western blot; human; fig 7h
Abcam L1CAM antibody (Abcam, ab24345) was used in western blot on human samples (fig 7h). Cancer Cell (2017) ncbi
mouse monoclonal (UJ127.11)
  • immunoprecipitation; human; loading ...; fig 7d
  • western blot; human; fig 6b
Abcam L1CAM antibody (Abcam, ab20148) was used in immunoprecipitation on human samples (fig 7d) and in western blot on human samples (fig 6b). Cancer Cell (2017) ncbi
mouse monoclonal (2C2)
  • immunocytochemistry; mouse; 1:500; fig 8
Abcam L1CAM antibody (Abcam, ab24345) was used in immunocytochemistry on mouse samples at 1:500 (fig 8). Sci Rep (2016) ncbi
mouse monoclonal (5G3)
  • flow cytometry; human; 1:100; fig 3d
  • immunocytochemistry; human; 1:50; fig 3e
Abcam L1CAM antibody (Abcam, ab95694) was used in flow cytometry on human samples at 1:100 (fig 3d) and in immunocytochemistry on human samples at 1:50 (fig 3e). PLoS ONE (2015) ncbi
mouse monoclonal (UJ127.11)
  • immunocytochemistry; human; 1:100; fig 2
Abcam L1CAM antibody (Abcam, UJ127.11) was used in immunocytochemistry on human samples at 1:100 (fig 2). Int J Oncol (2015) ncbi
mouse monoclonal (2C2)
  • flow cytometry; mouse; 1:300
Abcam L1CAM antibody (Abcam, ab24345) was used in flow cytometry on mouse samples at 1:300. PLoS ONE (2013) ncbi
mouse monoclonal (2C2)
  • immunohistochemistry - frozen section; rat; 1:300
Abcam L1CAM antibody (Abcam, ab24345) was used in immunohistochemistry - frozen section on rat samples at 1:300. J Comp Neurol (2011) ncbi
mouse monoclonal (UJ127.11)
  • immunohistochemistry - frozen section; rat; 1:100
Abcam L1CAM antibody (Abcam, ab20148) was used in immunohistochemistry - frozen section on rat samples at 1:100. J Comp Neurol (2011) ncbi
Santa Cruz Biotechnology
mouse monoclonal (UJ127.11)
  • western blot; human; 1:1000; loading ...; fig 1e
Santa Cruz Biotechnology L1CAM antibody (Santa Cruz, sc-53386) was used in western blot on human samples at 1:1000 (fig 1e). Nat Commun (2021) ncbi
mouse monoclonal (D-5)
  • western blot; human; 1:1000; loading ...; fig 2b
Santa Cruz Biotechnology L1CAM antibody (Santa Cruz, sc-374046) was used in western blot on human samples at 1:1000 (fig 2b). Sci Rep (2016) ncbi
BioLegend
mouse monoclonal (14.10)
  • other; human; loading ...; fig 4c
BioLegend L1CAM antibody (BioLegend, 826701) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
mouse monoclonal (14.10)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; tbl 1
BioLegend L1CAM antibody (Biolegend, 14.10) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 1). Br J Cancer (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D5N9S)
  • western blot; human; fig 7a
Cell Signaling Technology L1CAM antibody (Cell Signaling, 89861S) was used in western blot on human samples (fig 7a). Cell Rep (2019) ncbi
MilliporeSigma
mouse monoclonal (UJ127.11)
  • western blot; human; 1:1000; loading ...; fig 8a
MilliporeSigma L1CAM antibody (Sigma-Aldrich, L4543) was used in western blot on human samples at 1:1000 (fig 8a). Int J Mol Sci (2022) ncbi
mouse monoclonal (UJ127.11)
  • western blot; human; loading ...; fig 5e
MilliporeSigma L1CAM antibody (Sigma, L4543) was used in western blot on human samples (fig 5e). Cell (2019) ncbi
mouse monoclonal (UJ127.11)
  • western blot knockout validation; human; fig 10
In order to elucidate the mechanisms by which L1CAM gene mutations result in neuronal defects, MilliporeSigma L1CAM antibody (Sigma-Aldrich, UJ127.11) was used in western blot knockout validation on human samples (fig 10). J Exp Med (2016) ncbi
mouse monoclonal (UJ127.11)
  • blocking or activating experiments; human
  • western blot; human; 1:1000; fig 4a
MilliporeSigma L1CAM antibody (Sigma-Aldrich, L4543) was used in blocking or activating experiments on human samples and in western blot on human samples at 1:1000 (fig 4a). PLoS ONE (2015) ncbi
Articles Reviewed
  1. Van Meenen D, Doege A, Alefeld E, Haase A, Beier M, Kiefer T, et al. ADAM10 and ADAM17-Novel Players in Retinoblastoma Carcinogenesis. Int J Mol Sci. 2022;23: pubmed publisher
  2. Shiwaku H, Katayama S, Kondo K, Nakano Y, Tanaka H, Yoshioka Y, et al. Autoantibodies against NCAM1 from patients with schizophrenia cause schizophrenia-related behavior and changes in synapses in mice. Cell Rep Med. 2022;3:100597 pubmed publisher
  3. Nam J, Kim A, Choi S, Kim J, Choi K, Cho S, et al. An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage. Nat Commun. 2021;12:3279 pubmed publisher
  4. Hoj J, Mayro B, Pendergast A. A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis. Cell Rep. 2019;29:3421-3434.e8 pubmed publisher
  5. Valentiner U, Knips J, Pries R, Clauditz T, Münscher A, Sauter G, et al. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma. Cancers (Basel). 2019;11: pubmed publisher
  6. Patzke C, Brockmann M, Dai J, Gan K, Grauel M, Fenske P, et al. Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses. Cell. 2019;179:498-513.e22 pubmed publisher
  7. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  8. Agrawal P, Fontanals Cirera B, Sokolova E, Jacob S, Vaiana C, Argibay D, et al. A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis. Cancer Cell. 2017;31:804-819.e7 pubmed publisher
  9. Roberts C, Tran M, Pitruzzello M, Wen W, Loeza J, Dellinger T, et al. TWIST1 drives cisplatin resistance and cell survival in an ovarian cancer model, via upregulation of GAS6, L1CAM, and Akt signalling. Sci Rep. 2016;6:37652 pubmed publisher
  10. van der Putten L, Visser N, Van De Vijver K, Santacana M, Bronsert P, Bulten J, et al. L1CAM expression in endometrial carcinomas: an ENITEC collaboration study. Br J Cancer. 2016;115:716-24 pubmed publisher
  11. Baleriola J, Álvarez Lindo N, de la Villa P, Bernad A, Blanco L, Suárez T, et al. Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina. Sci Rep. 2016;6:25928 pubmed publisher
  12. Patzke C, Acuna C, Giam L, Wernig M, Südhof T. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation. J Exp Med. 2016;213:499-515 pubmed publisher
  13. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  14. Lund K, Dembinski J, Solberg N, Urbanucci A, Mills I, Krauss S. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS ONE. 2015;10:e0123684 pubmed publisher
  15. Kubelt C, Hattermann K, Sebens S, Mehdorn H, Held Feindt J. Epithelial-to-mesenchymal transition in paired human primary and recurrent glioblastomas. Int J Oncol. 2015;46:2515-25 pubmed publisher
  16. Geels Y, van der Putten L, van Tilborg A, Lurkin I, Zwarthoff E, Pijnenborg J, et al. Immunohistochemical and genetic profiles of endometrioid endometrial carcinoma arising from atrophic endometrium. Gynecol Oncol. 2015;137:245-51 pubmed publisher
  17. Kapogiannis D, Boxer A, Schwartz J, Abner E, Biragyn A, Masharani U, et al. Dysfunctionally phosphorylated type 1 insulin receptor substrate in neural-derived blood exosomes of preclinical Alzheimer's disease. FASEB J. 2015;29:589-96 pubmed publisher
  18. Sung S, Wu I, Chuang P, Petros J, Wu H, Zeng H, et al. Targeting L1 cell adhesion molecule expression using liposome-encapsulated siRNA suppresses prostate cancer bone metastasis and growth. Oncotarget. 2014;5:9911-29 pubmed
  19. Ghazvini M, Sonneveld P, Kremer A, Franken P, Sacchetti A, Atlasi Y, et al. Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. PLoS ONE. 2013;8:e73872 pubmed publisher
  20. Matsunaga E, Nambu S, Iriki A, Okanoya K. Expression pattern of cadherins in the naked mole rat (Heterocephalus glaber) suggests innate cortical diversification of the cerebrum. J Comp Neurol. 2011;519:1736-47 pubmed publisher
  21. Silletti S, Yebra M, Perez B, Cirulli V, McMahon M, Montgomery A. Extracellular signal-regulated kinase (ERK)-dependent gene expression contributes to L1 cell adhesion molecule-dependent motility and invasion. J Biol Chem. 2004;279:28880-8 pubmed