This is a Validated Antibody Database (VAD) review about human KIF11, based on 10 published articles (read how Labome selects the articles), using KIF11 antibody in all methods. It is aimed to help Labome visitors find the most suited KIF11 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
KIF11 synonym: EG5; HKSP; KNSL1; MCLMR; TRIP5; kinesin-like protein KIF11; TR-interacting protein 5; TRIP-5; kinesin-like protein 1; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor-interacting protein 5

Abcam
mouse monoclonal (mAbcam 51976)
  • immunocytochemistry; human; loading ...; fig 1e
In order to assess the effect of three alpha-tubulin acetyltransferase 1-specific shRNAs on proliferation and morphology in HeLa and A549 cell lines, Abcam KIF11 antibody (Abcam, ab51976) was used in immunocytochemistry on human samples (fig 1e). Cell Death Discov (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 4
  • western blot; human; 1:500; fig s1
In order to elucidate the spindle proteome that is clathirin-dependent, Abcam KIF11 antibody (Abcam, ab137535) was used in immunocytochemistry on human samples at 1:100 (fig 4) and in western blot on human samples at 1:500 (fig s1). Mol Cell Proteomics (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-1)
  • immunocytochemistry; human; loading ...; fig 3
Santa Cruz Biotechnology KIF11 antibody (Santa Cruz, sc-365681) was used in immunocytochemistry on human samples (fig 3). Int J Oncol (2017) ncbi
OriGene
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 2d
  • western blot; mouse; 1:100; loading ...; fig 2e
OriGene KIF11 antibody (Origene, TA301478) was used in immunocytochemistry on mouse samples at 1:100 (fig 2d) and in western blot on mouse samples at 1:100 (fig 2e). Science (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig s3
  • western blot; human; 1:100; fig s3
  • immunoprecipitation; mouse; 1:100; fig 3
  • immunocytochemistry; mouse; 1:100; fig 3
  • western blot; mouse; 1:100; fig 3
OriGene KIF11 antibody (Origene, TA301478) was used in immunocytochemistry on human samples at 1:100 (fig s3), in western blot on human samples at 1:100 (fig s3), in immunoprecipitation on mouse samples at 1:100 (fig 3), in immunocytochemistry on mouse samples at 1:100 (fig 3) and in western blot on mouse samples at 1:100 (fig 3). Nat Cell Biol (2016) ncbi
Novus Biologicals
rabbit polyclonal
  • immunocytochemistry; human
Novus Biologicals KIF11 antibody (Novus biologicals, NB500-181) was used in immunocytochemistry on human samples . Peerj (2014) ncbi
MilliporeSigma
rabbit polyclonal
  • immunocytochemistry; human
MilliporeSigma KIF11 antibody (Sigma-Aldrich, HPA010568) was used in immunocytochemistry on human samples . Oncogenesis (2014) ncbi
Cell Signaling Technology
mouse monoclonal (4H3-1F12)
  • western blot; human; loading ...; fig 2b
Cell Signaling Technology KIF11 antibody (Cell Signaling, 4203) was used in western blot on human samples (fig 2b). Oncogene (2018) ncbi
BD Biosciences
mouse monoclonal (20/EG5)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 3a
BD Biosciences KIF11 antibody (BD Biosciences, 611187) was used in immunohistochemistry on mouse samples at 1:1000 (fig 3a). Nat Commun (2018) ncbi
mouse monoclonal (20/EG5)
  • immunocytochemistry; human
In order to identify the role of PCTAIRE1 in cancer cells, BD Biosciences KIF11 antibody (BD, 611186) was used in immunocytochemistry on human samples . Cancer Res (2014) ncbi
Articles Reviewed
  1. Freixo F, Martínez Delgado P, Manso Y, Sánchez Huertas C, Lacasa C, Soriano E, et al. NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nat Commun. 2018;9:2330 pubmed publisher
  2. Zhang F, Virshup D, Cheong J. Oncogenic RAS-induced CK1α drives nuclear FOXO proteolysis. Oncogene. 2018;37:363-376 pubmed publisher
  3. Schneider M, Christopoulos P, Muley T, Warth A, Klingmueller U, Thomas M, et al. AURKA, DLGAP5, TPX2, KIF11 and CKAP5: Five specific mitosis-associated genes correlate with poor prognosis for non-small cell lung cancer patients. Int J Oncol. 2017;50:365-372 pubmed publisher
  4. Kanakkanthara A, Jeganathan K, Limzerwala J, Baker D, Hamada M, Nam H, et al. Cyclin A2 is an RNA binding protein that controls Mre11 mRNA translation. Science. 2016;353:1549-1552 pubmed
  5. Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed publisher
  6. van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;18:814-21 pubmed publisher
  7. Rao S, Flores Rodriguez N, Page S, Wong C, Robinson P, Chircop M. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics. 2016;15:2537-53 pubmed publisher
  8. Wilson P, Payne T. Genetic reprogramming of human amniotic cells with episomal vectors: neural rosettes as sentinels in candidate selection for validation assays. Peerj. 2014;2:e668 pubmed publisher
  9. Yanagi T, Krajewska M, Matsuzawa S, Reed J. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer Res. 2014;74:5795-807 pubmed publisher
  10. Kuga T, Nie H, Kazami T, Satoh M, Matsushita K, Nomura F, et al. Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation. Oncogenesis. 2014;3:e94 pubmed publisher